Riset dan Pengabdian Masyarakat
Do Research Sosial Service and Innovation
Publikasi Scopus 2024 per tanggal 30 November 2024 (994 artikel)
Publikasi Scopus 2024 per tanggal 30 November 2024 (994 artikel)
Tandean, Steven (57200655152); Japardi, Iskandar (57200649149); Rusda, Muhammad (57201675676); Indharty, Rr Suzy (57192909801); Lelo, Aznan (59195385200); Aman, Renindra Ananda (36848942500); Amin, Mustafa Mahmud (57201668200); Siahaan, Andre Marolop Pangihutan (57201669352); Eyanoer, Putri Chairani (45561124000); D'Prinzessin, Celine Augla (59196009500); Lesmana, Ronny (55363079700); Popova, Milena (7103172058); Trusheva, Boryana (8542765200); Bankova, Vassya (35479237300); Zulhendri, Felix (49
Chemical Composition and Neuroprotective Properties of Indonesian Stingless Bee (Geniotrigona thoracica) Propolis Extract in an In-Vivo Model of Intracerebral Hemorrhage (ICH)
2024
Nutrients
16
12
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85197176112&doi=10.3390%2fnu16121880&partnerID=40&md5=c2b0f17a5747aa84f10408453a771fc6
Philosophy Doctor in Medicine Programme, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia; Department of Neurosurgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia; Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia; Department of Neurosurgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, 10430, Jakarta, Indonesia; Department of Psychiatry, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia; Department of Community and Preventive Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia; Undergraduate Program in Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia; Physiology Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Kabupaten Sumedang, 45363, West Java, Indonesia; Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Kabupaten Sumedang, Jawa Barat 45363, Indonesia; Kebun Efi, Kabupaten Karo, Sumatera Utara 22171, Indonesia
Stroke is the world's second-leading cause of death. Current treatments for cerebral edema following intracerebral hemorrhage (ICH) mainly involve hyperosmolar fluids, but this approach is often inadequate. Propolis, known for its various beneficial properties, especially antioxidant and anti-inflammatory properties, could potentially act as an adjunctive therapy and help alleviate stroke-associated injuries. The chemical composition of Geniotrigona thoracica propolis extract was analyzed by GC-MS after derivatization for its total phenolic and total flavonoid content. The total phenolic content and total flavonoid content of the propolis extract were 1037.31 ± 24.10 μg GAE/mL and 374.02 ± 3.36 μg QE/mL, respectively. By GC-MS analysis, its major constituents were found to be triterpenoids (22.4% of TIC). Minor compounds, such as phenolic lipids (6.7% of TIC, GC-MS) and diterpenic acids (2.3% of TIC, GC-MS), were also found. Ninety-six Sprague Dawley rats were divided into six groups; namely, the control group, the ICH group, and four ICH groups that received the following therapies: mannitol, propolis extract (daily oral propolis administration after the ICH induction), propolis-M (propolis and mannitol), and propolis-B+A (daily oral propolis administration 7 days prior to and 72 h after the ICH induction). Neurocognitive functions of the rats were analyzed using the rotarod challenge and Morris water maze. In addition, the expression of NF-κB, SUR1-TRPM4, MMP-9, and Aquaporin-4 was analyzed using immunohistochemical methods. A TUNEL assay was used to assess the percentage of apoptotic cells. Mannitol significantly improved cognitive-motor functions in the ICH group, evidenced by improved rotarod and Morris water maze completion times, and lowered SUR-1 and Aquaporin-4 levels. It also significantly decreased cerebral edema by day 3. Similarly, propolis treatments (propolis-A and propolis-B+A) showed comparable improvements in these tests and reduced edema. Moreover, combining propolis with mannitol (propolis-M) further enhanced these effects, particularly in reducing edema and the Virchow-Robin space. These findings highlight the potential of propolis from the Indonesian stingless bee, Geniotrigona thoracica, from the Central Tapanuli region as a neuroprotective, adjunctive therapy.
Animals; Antioxidants; Bees; Brain Edema; Cerebral Hemorrhage; Disease Models, Animal; Flavonoids; Gas Chromatography-Mass Spectrometry; Male; Neuroprotective Agents; Phenols; Propolis; Rats; Rats, Sprague-Dawley; antioxidant; flavonoid; neuroprotective agent; phenol derivative; propolis; animal; bee; brain edema; brain hemorrhage; chemistry; disease model; drug therapy; male; mass fragmentography; rat; Sprague Dawley rat
20726643
38931235
Article
Q1
1301
2867