Riset dan Pengabdian Masyarakat
Do Research Sosial Service and Innovation
Publikasi Scopus 2024 per tanggal 30 September 2024 (820 artikel)
Publikasi Scopus 2024 per tanggal 30 September 2024 (820 artikel)
Subahar, Rizal (6508197883); Hadyansyah, Rizqy (59229205600); Aldilla, Rachmanin (59229088300); Yulhasri, Yulhasri (57225180520); Winita, Rawina (57220782528); Dwira, Surya (57204110404); El Bayani, Gulshan Fahmi (57195935050)
Toxicity of 6-gingerol and Cymbopogon citratus against Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae): Mortality, detoxifying enzymes, and morphological ultrastructure alterations in lice
2024
Research in Veterinary Science
177
105364
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85199254708&doi=10.1016%2fj.rvsc.2024.105364&partnerID=40&md5=5a3d6ac21849f052aa2759615983adda
Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Medical Doctor Program, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Medical Physiology and Biophysics, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia
Pediculus humanus capitis (head louse), which causes pediculosis capitis, remains a global health concern. Plant products are efficient alternative pediculicides for treating the human ectoparasite P. h. capitis which is resistant to permethrin. The study evaluates the toxicity and mechanisms of 6-gingerol and Cymbopogon citratus leaf extract on P. h. capitis. Pediculus humanus capitis adult stages were exposed to three different dosages of 6-gingerol and C. citratus crude leaf extract on filter sheets for 5, 10, and 30 min, respectively. The biochemical approach was used to assess the activity of detoxifying enzymes including acetylcholinesterase (AChE), glutathione S-transferase (GST), and oxidase. Scanning electron microscope (SEM) was used to investigate the ultrastructure of the morphological body of lice. After 30 min, 6-gingerol and C. citratus leaf extract killed P. h. capitis completely. Bioassay periods significantly affected lice mortality (P < 0.05). The LC50 values for 6-gingerol and C. citratus extract were 1.79 μg/cm2 and 25.0 μg/cm2, respectively. 6-Gingerol and C. citratus leaf extract significantly lower AChE and GST activity (P < 0.05). Cymbopogon citratus also caused morphological ultrastructure changes in P. h. capitis, including an irregularly formed head, thorax, abdominal respiratory spiracles, and belly. 6-Gingerol and C. citratus leaf extracts could be used as an alternate pediculicide to decrease P. h. capitis populations. © 2024
(6) gingerol; Cymbopogon citratus extract; Article; biochemical analysis; chemical structure; controlled study; enzyme activity; exposure; imago; LC50; LC90; LT50; LT90; mortality; nonhuman; Pediculus humanus capitis; plant leaf; scanning electron microscopy; ultrastructure
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia; Universitas Indonesia, UI, (HIBAH PUTI Q2 2020); Universitas Indonesia, UI
Funding text 1: This research was funded by the Directorate of Research and Community Service at the Universitas Indonesia (grant no. HIBAH PUTI Q2 2020).This research was funded by theThe Directorate of Research and Development at the Universitas Indonesia (grant number HIBAH PUTI Q2 2020). The authors would like to thank the Head of the Department of Parasitology and the Head of the Department o
Elsevier B.V.
00345288
Article
Q1
617
8656