Riset dan Pengabdian Masyarakat
Do Research Sosial Service and Innovation
Publikasi Scopus 2024 per tanggal 30 September 2024 (820 artikel)
Publikasi Scopus 2024 per tanggal 30 September 2024 (820 artikel)
Basalamah, Faris (57192972927); Dilogo, Ismail Hadisoebroto (56161962800); Raharjo, Sunu Budhi (57017880700); Mansyur, Muchtaruddin (37085506800); Siregar, Nuryati Chairani (6508087790); Ibrahim, Nurhadi (56609777400); Setianto, Budi Yuli (36469084300); Yuniadi, Yoga (57155066100)
TBX3 transfection and nodal signal pathway inhibition promote differentiation of adipose mesenchymal stem cell to cardiac pacemaker-like cells
2024
Stem Cell Research and Therapy
15
1
148
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85193715405&doi=10.1186%2fs13287-024-03760-x&partnerID=40&md5=178de8109665d59869b772aa68489b0a
Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Faculty of Medicine and Health, University Muhammadiyah Jakarta, Banten, 15419, Indonesia; Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia-National Heart Center Harapan Kita, Jakarta, 10430, Indonesia; Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Indonesia-Dr Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Stem Cell and Tissue Engineering Research Cluster, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Dr Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Medical Physiology and Biophysics, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Neuroscience and Brain Development Research Cluster, Indonesian Medical Education and Researsch Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Cardiology and Vascular Medicine, Faculty of Medicine, Health and Nursing, Universitas Gajah Mada, Yogyakarta, 55281, Indonesia; Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia-RS Jantung Dan Pembuluh Darah Harapan Kita, Jakarta, 10420, Indonesia
Background: Mesenchymal stem cells (MSCs) are known as one of the best candidate cells to produce cardiac pacemaker-like cells (CPLCs). Upregulation of TBX3 transcription factor and inhibition of the nodal signal pathway have a significant role in the formation of cardiac pacemaker cells such as sinoatrial and atrioventricular nodes, which initiate the heartbeat and control the rhythm of heart contractions. This study aimed to confirm the effects of transfection of TBX3 transcription factor and inhibition of the nodal signal pathway on differentiating adipose-derived MSCs (AD-MSCs) to CPLCs. AD-MSCs were characterized using flow cytometry and three-lineage differentiation staining. Methods: The transfection of TBX3 plasmid was carried out using lipofectamine, and inhibition of the nodal signal pathway was done using the small-molecule SB431542. The morphology of the cells was observed using a light microscope. Pacemaker-specific markers, including TBX3, Cx30, HCN4, HCN1, HCN3, and KCNN4, were evaluated using the qRT-PCR method. For protein level, TBX3 and Cx30 were evaluated using ELISA and immunofluorescence staining. The electrophysiology of cells was evaluated using a patch clamp. Results: The TBX3 expression in the TBX3, SM, and TBX + SM groups significantly higher (p < 0.05) compared to the control group and cardiomyocytes. The expression of Cx40 and Cx43 genes were lower in TBX3, SM, TBX + SM groups. In contrast, Cx30 gene showed higher expression in TBX3 group. The expression HCN1, HCN3, and HCN4 genes are higher in TBX3 group. Conclusion: The transfection of TBX3 and inhibition of the nodal signal pathway by small-molecule SB431542 enhanced differentiation of AD-MSCs to CPLCs. © The Author(s) 2024.
Adipose Tissue; Cell Differentiation; Cells, Cultured; Humans; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; Mesenchymal Stem Cells; Myocytes, Cardiac; Signal Transduction; T-Box Domain Proteins; Transfection; 5' nucleotidase; complementary DNA; endoglin; lipofectamine; TBX3 transcription factor; Thy 1 membrane glycoprotein; transcription factor; unclassified drug; hyperpolarization activated cyclic nucleotide gated channel; T box transcription factor; TBX3 protein, human; adipose-derived mesenchymal stem cell; adult; Article; cardiac pacemaker like cell; cardiac stem cell; cell culture; cell differentiation; cell isolation; cell structure; Cx30 gene; Cx40 gene; Cx43 gene; electrophysiology; enzyme linked immunosorbent assay; flow cytometry; gene; gene expression; genetic t
IMERI; Fakultas Kedokteran, Universitas Indonesia, FKUI
The authors would like to especially thank Stem Cells and Tissue Engineering (SCTE), IMERI, Faculty of Medicine, Universitas Indonesia for providing necessary facilities and support to optimize and complete this study.
BioMed Central Ltd
17576512
38778426
Article
Q1
1798
1641