Riset dan Pengabdian Masyarakat
Do Research Sosial Service and Innovation
Publikasi Scopus 2024 per tanggal 30 September 2024 (820 artikel)
Publikasi Scopus 2024 per tanggal 30 September 2024 (820 artikel)
Maladan, Yustinus (57204842592); Retnaningrum, Endah (57190936609); Daryono, Budi Setiadi (8928730200); Salsabila, Korrie (57192272141); Sarassari, Rosantia (57204038282); Khoeri, Miftahuddin Majid (55994827400); Sari, Ratna Fathma (58918622800); Balqis, Sarah Azhari (58919511400); Wahid, Ghina Athyah (58919958100); Safari, Dodi (23493586700)
Pneumococcal transposon profiling associated with macrolide, tetracycline, and chloramphenicol resistance from carriage isolates of serotype 19F in Indonesia
2024
Infection, Genetics and Evolution
125
105672
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204734328&doi=10.1016%2fj.meegid.2024.105672&partnerID=40&md5=e6393c21b6a910438de2c90f5ad4c0ae
Biology Doctoral Students, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia; Microbiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia; Genetics Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia; Eijkman Research Center for Molecular Biology, The National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia; Doctoral program in Biomedical, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
Genetic evolution of resistance due to mutations and transposon insertions is the primary cause of antimicrobial resistance in Streptococcus pneumoniae. Resistance to macrolide, tetracycline, and chloramphenicol is caused by the insertion of specific genes that carried by transposon (Tn). This study aims to analyze transposon profiling associated with macrolide, tetracycline, and chloramphenicol resistance from carriage isolates of S. pneumoniae serotype 19F in Indonesia. S. pneumoniae serotype 19F isolates were collected from nasopharyngeal swab specimens from different regions in Indonesia. Genomic DNA was extracted from sixteen isolates and whole genome sequencing was performed on Illumina platform. Raw sequence data were analyzed using de novo assembly by ASA3P and Microscope server. The presence of transposons was identified with detection of int and xis genes and visualized by pyGenomeViz. The genome size of S. pneumoniae ranges from 2,040,117 bp to 2,437,939 bp, with a GC content of around 39 %. ST1464 (4/16) and ST271 (3/16) were found as the predominant sequence type among isolates. Tn2010 was the most common transposon among S. pneumoniae serotype 19F isolates (7/16) followed by Tn2009 (4/16), and Tn5253 (3/16). We identified two deletion sites within the tetM gene (2 bp and 58 bp) that confer tetracycline susceptibility from one isolate. This study suggests that genomic analysis can be employed for the detection and surveillance of antimicrobial resistance genes among S. pneumoniae strains isolated from various regions in Indonesia. © 2024
chloramphenicol; clindamycin; cytosine; erythromycin; genomic DNA; guanine; macrolide; tetracycline; adult; antibiotic sensitivity; Article; bacterial genome; bacterial strain; bacterium carrier; bacterium isolate; child; chloramphenicol resistance; controlled study; DNA content; DNA extraction; gene; gene deletion; genome analysis; genome size; genomics; human; illumina sequencing; Indonesia; int gene; macrolide resistance; nasopharyngeal swab; nonhuman; serotype; Streptococcus pneumoniae; Streptococcus pneumoniae serotype 19F; tetM gene; tetracycline resistance; transposon; whole genome sequencing; xis gene
Elsevier B.V.
15671348
39313084
Article
Q1
718
7243