Publikasi Scopus 2024 per tanggal 31 Januari 2024 (57 artikel)

Subahar R.; Huang A.; Wijaya R.S.; Nur L.S.E.; Susanto L.; Firmansyah N.E.; Yulhasri Y.; El Bayani G.F.; Dwira S.
Subahar, Rizal (6508197883); Huang, Ayleen (58654515300); Wijaya, Rafaella Shiene (57989723800); Nur, Lia Savitri Eka (58654567600); Susanto, Lisawati (6507704130); Firmansyah, Nurhadi Eko (57212586145); Yulhasri, Yulhasri (57225180520); El Bayani, Gulshan Fahmi (57195935050); Dwira, Surya (57204110404)
6508197883; 58654515300; 57989723800; 58654567600; 6507704130; 57212586145; 57225180520; 57195935050; 57204110404
First report on evaluation of commercial eugenol and piperine against Aedes aegypti L (Diptera: Culicidae) larvae: Mortality, detoxifying enzyme, and histopathological changes in the midgut
2024
Parasitology International
98
102813
0
Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Medical Doctor Program, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Medical Physiology and Biophysics, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia
Subahar R., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Huang A., Medical Doctor Program, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Wijaya R.S., Medical Doctor Program, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Nur L.S.E., Medical Doctor Program, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Susanto L., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Firmansyah N.E., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Yulhasri Y., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; El Bayani G.F., Department of Medical Physiology and Biophysics, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Dwira S., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia
Dengue fever is a worldwide public health problem, and efforts to eradicate it have focused on controlling the dengue vector, Aedes aegypti. This study aims to assess the toxicity and effect of commercial eugenol and piperine on Ae. aegypti larvae through enzyme detoxification and histopathological changes in the midgut. Laboratory-reared Ae. aegypti larvae were treated with various concentrations of commercial eugenol and piperine and observed after 24, 48, and 72 h. Biochemical methods were used to assess detoxification enzyme activity for acetylcholinesterase, glutathione S-transferase, and oxidase, and changes in the midgut were examined using routine histological examination. In terms of larvicidal activity, piperine exceeded eugenol. Piperine and eugenol had LC50 and LC90 values of 3.057 and 5.543 μM, respectively, and 6.421 and 44.722 μM at 24 h. Piperine and eugenol reduced oxidase activity significantly (p < 0.05), but increased acetylcholinesterase and glutathione S-transferase activity significantly (p < 0.05). After being exposed to piperine and eugenol, the food bolus and peritrophic membrane ruptured, the epithelial layer was interrupted and irregular, the epithelial cells shrank and formed irregularly, and the microvilli became irregular in shape. Commercial piperine and eugenol behave as potential larvicides, with processes involving altered detoxifying enzymes, specifically decreased oxidase function and increased GST activity, as well as midgut histological abnormalities. © 2023
Aedes aegypti; Detoxification enzyme; Eugenol; Histopathological midgut; Piperine
Acetylcholinesterase; Aedes; Animals; Eugenol; Glutathione Transferase; Insecticides; Larva; Mosquito Vectors; Oxidoreductases; Plant Extracts; acetylcholinesterase; eugenol; glutathione transferase; insecticide; oxidoreductase; piperine; plant extract; Aedes; animal; larva; mosquito vector
Universitas Indonesia, UI; Direktorat Riset and Pengembangan, Universitas Indonesia, DRPM UI, (HIBA UI Q1-Q2 2019)
Funding text 1: This research was funded by the Directorate of Research and Community Service at the University of Indonesia (grant number HIBA UI Q1-Q2 2019). The authors would like to thank the Head of the Department of Parasitology and the Head of the Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, for providing necessary facilities for this study
Elsevier Ireland Ltd
13835769
37793471
Article
Q2
557
9287