Publikasi Scopus 2024 per tanggal 31 Juli 2024 (607 artikel)

Hansur L.; Louisa M.; Ernawaty B.; Wuyung P.E.; Zaini J.; Fadillah F.; Wibowo H.
Hansur, Lismayana (57296411600); Louisa, Melva (41461551400); Ernawaty, Beti (58952234700); Wuyung, Puspita Eka (57192889605); Zaini, Jamal (57221833355); Fadillah, Fadillah (57879623800); Wibowo, Heri (57217690943)
57296411600; 41461551400; 58952234700; 57192889605; 57221833355; 57879623800; 57217690943
Histopathology Assay of The Lung After Intratracheal Injection of SARS-CoV-2 Spike Protein Recombinant in Mice: A Preliminary Study
2024
AIP Conference Proceedings
3080
1
90002
0
Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Jl. Sultan Alauddin No. 259, Gn. Sari, Kec. Rappocini, South Sulawesi, Makassar, 90221, Indonesia; Department of Pharmacology & Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Department of Pathological Anatomy, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Animal Research Facility, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia
Hansur L., Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Jl. Sultan Alauddin No. 259, Gn. Sari, Kec. Rappocini, South Sulawesi, Makassar, 90221, Indonesia; Louisa M., Department of Pharmacology & Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Ernawaty B., Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Wuyung P.E., Department of Pathological Anatomy, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia, Animal Research Facility, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Zaini J., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Fadillah F., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia; Wibowo H., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya no 6, Jakarta, 10430, Indonesia
COVID-19 can cause ARDS, characterized by Diffuse alveolar Damage (DAD) generated by cell death, surfactants, and proteins to the alveolar. When the hyaline membrane accumulates or thickens, oxygen exchange in the alveoli is disrupted. Recent research employing animal model mice has been designed and carried out on various methods that could be used to investigate the mechanisms of inflammation and infection in COVID-19 disease. In this study, we injected SARS-CoV-2 spike recombinant protein into the trachea to make an animal model for studying DAD and infiltration with a histological assay. Methods: To initiate mouse models, an incision is made in above the trachea, the muscles and glands above the trachea are moved. A 26-gauge needle delivered 15 µg of SARS-CoV-2 recombinant spike protein (SC-2 RSP) in 50 µl saline, followed by a 100 µl air injection. The control group was given 50 µl saline intratracheally. Afterward, mice were euthanized with intraperitoneal injections of ketamine and xylazine at 1, 2, and 7-days post-injection (d.p.i.) to harvest the lungs. Hematoxylin & Eosin were used to stain lung tissue for histological examinations. Result: Lung histopathology of BALB/c mice injected with SARS-CoV-2 recombinant spike protein at 1-, 2-, and 7-days post instillation showed mild immune cell infiltration. Cell infiltration was also found at alveolar, perivascular, and peribronchiolar locations compared with untreated mice. According to our study, SARS-CoV-2 recombinant spike protein has been shown to trigger mild lung inflammation in BALB/c mice. © 2024 American Institute of Physics Inc.. All rights reserved.
BALB/c; COVID-19; inflammation; intratracheal; pulmonary disease
American Institute of Physics
0094243X
Conference paper
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164
21059