Publikasi Scopus 2024 per tanggal 31 Maret 2024 (233 artikel)

Margiana R.; Gupta R.; Al-Jewari W.M.; Hjazi A.; Alsaab H.O.; Mustafa Y.F.; Singh R.; Thaibt R.; Alkhayyat S.; Ibrahim A.J.
Margiana, Ria (56685900600); Gupta, Reena (55488123300); Al-Jewari, Watheq Mohammed (57207993507); Hjazi, Ahmed (57226852880); Alsaab, Hashem O. (56479366700); Mustafa, Yasser Fakri (57203725947); Singh, Rajesh (58528479900); Thaibt, Ruiscul (58860800100); Alkhayyat, Safa (58286050400); Ibrahim, Ahmed Jaber (57383064400)
56685900600; 55488123300; 57207993507; 57226852880; 56479366700; 57203725947; 58528479900; 58860800100; 58286050400; 57383064400
Evaluation of telomere length, reactive oxygen species, and apoptosis in spermatozoa of patients with oligospermia
2024
Cell Biochemistry and Function
42
2
e3935
0
Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Master's Programme Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Andrology Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Institute of Pharmaceutical Research, GLA University, Bharthia, India; Department of Medical Technology, Al Rafidain University College, Bagdad, Iraq; Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia; Pharmaceutics and Pharmaceutical Technology, Taif University, Taif, Saudi Arabia; Department of Pharmaceutical Chemistry, University of Mosul, Mosul, Iraq; Uttaranchal Institute of Technology, Uttaranchal University, Dehradun, India; Medical Technical College, Al-Farahidi University, Baghdad, Iraq; College of Pharmacy, The Islamic University, Najaf, Iraq; Scientific Research Center, Al-Ayen University, Nasiriyah, Iraq
Margiana R., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Master's Programme Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Andrology Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Gupta R., Institute of Pharmaceutical Research, GLA University, Bharthia, India; Al-Jewari W.M., Department of Medical Technology, Al Rafidain University College, Bagdad, Iraq; Hjazi A., Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia; Alsaab H.O., Pharmaceutics and Pharmaceutical Technology, Taif University, Taif, Saudi Arabia; Mustafa Y.F., Department of Pharmaceutical Chemistry, University of Mosul, Mosul, Iraq; Singh R., Uttaranchal Institute of Technology, Uttaranchal University, Dehradun, India; Thaibt R., Medical Technical College, Al-Farahidi University, Baghdad, Iraq; Alkhayyat S., College of Pharmacy, The Islamic University, Najaf, Iraq; Ibrahim A.J., Scientific Research Center, Al-Ayen University, Nasiriyah, Iraq
50% of cases of infertility are caused by male factor, which acquired or congenital problems may bring on. Male infertility can be caused by oligospermia and asthenozoospermia, which are common. Since the same mutations that cause azoospermia in some people also cause oligozoospermia in others, oligozoospermia may be thought of as a less severe form of azoospermia. Studies have demonstrated telomere length, catalase activity, super oxide dismutase (SOD), and DNA fragmentation can be influential factors for male infertility. The amount of apoptosis, oxidative stress factors, telomere length, and DNA fragmentation were some aspects of healthy sperm that we chose to look into in this study and compare to oligospermia individuals. Oligospermia patients (n = 24) and fertile men (n = 27) semen samples were collected, and the apoptosis rate of sperms in both groups was analyzed (Flow cytometry). Also, gene expression of apoptotic and antiapoptotic markers and telomere length were examined (real-time polymerase chain reaction). The sperm DNA fragmentation kit was used to determine DNA fragmentation and to evaluate catalase and SOD activity; the specific kits and methods were utilized. Higher expression levels of caspase3 (p =.0042), caspase8 (p =.0145), caspase9 (p =.0275), and BAX (p =.0202) mRNA were observed in patients who had oligospermia. In contrast, lower mRNA expression of BCL-2 (p =.0009) was detected in this group. In addition, telomere length was decreased in the oligospermia group (p <.0001) compared to the health group. Moreover, the frequency of apoptosis is induced in patients (p =.0026). The catalase activity is low (p =.0008), but the SOD activity is high (p =.0015) in the patient group. As a result of our findings, we may list the sperm cell apoptosis rate, telomere length, the degree of sperm DNA fragmentation, and lastly, the measurement of significant and efficient oxidative stress markers like SOD and catalase in semen plasma among the principal diagnostic characteristics for oligospermia. Future studies will be better able to treat oligospermia by showing whether these indicators are rising or falling. © 2024 John Wiley & Sons Ltd.
apoptosis; catalase and SOD activity; DNA fragmentation; oligospermia; spermatozoa; telomere length
Antioxidants; Apoptosis; Azoospermia; Catalase; DNA Fragmentation; Humans; Infertility, Male; Male; Oligospermia; Reactive Oxygen Species; RNA, Messenger; Semen; Spermatozoa; Superoxide Dismutase; Telomere; caspase 3; caspase 8; caspase 9; catalase; protein Bax; protein bcl 2; reactive oxygen metabolite; superoxide dismutase; antioxidant; catalase; messenger RNA; reactive oxygen metabolite; superoxide dismutase; adult; apoptosis; apoptosis rate; Article; clinical article; comparative study; controlled study; DNA fragmentation; flow cytometry; gene expression; human; male; male infertility; mRNA expression level; oligospermia; oxidative stress; protein expression level; real time polymerase chain reaction; sperm count; spermatozoon; spermatozoon motility; telomere length; apoptosis; azoospe
John Wiley and Sons Ltd
02636484
38379260
Article
Q2
753
6473