Riset dan Pengabdian Masyarakat
Do Research Sosial Service and Innovation
Publikasi Scopus 2024 per tanggal 31 Maret 2024 (233 artikel)
Publikasi Scopus 2024 per tanggal 31 Maret 2024 (233 artikel)
Lusiono, Pratiwi Rostiningtyas (58952064900); Sahlan, Muhamad (57189182661); Pramono, Andri Pramesyanti (8616373400); Pratami, Diah Kartika (57200370046); Pambudi, Sabar (37115903900); Nurhayati, Retno Wahyu (55748436600)
Differences in Treatment of Royal Jelly Powder by Freeze-drying as a Serum Substitute in Media Culture for Lymphocyte Cell
2024
AIP Conference Proceedings
3080
1
070004
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85188439029&doi=10.1063%2f5.0199153&partnerID=40&md5=093f15518e2993e571339d08822a1cb0
Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Department of Microbiology, Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, DKI Jakarta, 12450, Indonesia; Stem Cell and Tissue Engineering Research Center, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, DKI Jakarta, 12450, Indonesia; Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta, 12640, Indonesia; National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Centre for Vaccine and Drug Research, National Research and Innovation Agency Republic of Indonesia, Kawasan Puspiptek Serpong, Serpong, 15314, Indonesia; Stem Cell Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia
Fetal Bovine Serum (FBS), which offers nutrients for cell growth, has been the most widely used serum in proliferation studies. It does, however, necessitate screening because it contains unidentified elements, as well as viruses and prions, which pose a risk of infection. Apis mellifera royal jelly has the potential to replace FBS as a serum. In this study, a comparison of the effectiveness of three royal jelly treatments on lymphocyte cell proliferation will be performed: untreated royal jelly, soluble royal jelly, and hydrolyzed royal jelly, which is then freeze-dyed to generate a powdered product. Lymphocyte cells were cultured with various concentrations (2.5%, 5%, 7.5%, and 10%) and three different treatments of royal jelly. The results of these various concentrations were continued for up to 48 hours with continuous checking every 24 hours measured by the Microtetrazolium (MTT) assay. According to the results, lymphocyte cells cultured with the addition of a 2.5% concentration of untreated royal jelly Apis mellifera had significant differences, with the percent cell viability of 77.15% at 24 hours and 58.44% at 48 hours. This value was higher than that of soluble royal jelly (64.13% and 38.52%) and royal jelly hydrolyzate (71.08% and 54.59%) with the addition of the same concentration. © 2024 American Institute of Physics Inc.. All rights reserved.
American Institute of Physics
0094243X
Conference paper
-
164
21059