Publikasi Scopus 2024 per tanggal 31 Mei 2024 (409 artikel)

168

Authors

Chairunnisa S.; Mustopa A.Z.; Bela B.; Firdaus M.E.R.; Irawan S.; Arifah R.K.; Irawan H.; Nurfatwa M.; Umami R.N.; Ekawati N.; Hertati A.; Hasan N.

Author_Full_Names

Chairunnisa, Sheila (57539395900); Mustopa, Apon Zaenal (56781558900); Bela, Budiman (24723637900); Firdaus, Moh Egy Rahman (57203154080); Irawan, Shasmita (57218904860); Arifah, Rosyida Khusniatul (58286909200); Irawan, Herman (58650668500); Nurfatwa, Maritsa (57210969588); Umami, Rifqiyah Nur (55946476700); Ekawati, Nurlaili (57218911049); Hertati, Ai (57219869357); Hasan, Nurhasni (56600684900)

Author S Id

57539395900; 56781558900; 24723637900; 57203154080; 57218904860; 58286909200; 58650668500; 57210969588; 55946476700; 57218911049; 57219869357; 56600684900

Title

Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha

Year

2024

Source Title

Journal of Genetic Engineering and Biotechnology

Volume

Issue

Art No

100342

Page Start

Page End

Page Count

Cited By

Link

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184803938&doi=10.1016%2fj.jgeb.2023.100342&partnerID=40&md5=3a2e0797d0ae426e695bdd52847dc388

Affiliations

Master's Programme in Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Pharmacy, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km 10, Makassar, 90245, Indonesia

Authors With Affiliations

Chairunnisa S., Master's Programme in Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia, Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Mustopa A.Z., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Bela B., Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Firdaus M.E.R., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Irawan S., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Arifah R.K., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Irawan H., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Nurfatwa M., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Umami R.N., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Ekawati N., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Hertati A., Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Hasan N., Faculty of Pharmacy, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km 10, Makassar, 90245, Indonesia

Abstract

Background: Human papillomavirus (HPV) vaccination is one of the crucial national vaccination programs aimed at reducing the prevalence of the diseases associated with HPV infections, which continue to pose a global health concern. However, a significant disparity exists in the distribution of HPV vaccine, particularly in low-middle income countries where the cost of HPV vaccine becomes a major obstacle. Thus, it is essential to ensure the availability of an economically feasible HPV vaccine, necessitating immediate efforts to enhance the cost-effectiveness of vaccine production. This study aimed to develop an efficient production system for the recombinant HPV type 52 L1 protein as HPV vaccine material using methylotrophic yeast Hansenula polymorpha expression system. Results: This study presents an in-depth examination of the expression and scale-up production of HPV type 52 L1 protein using DASGIP® parallel bioreactor system. The pHIPX4 plasmid, which is regulated by the MOX promoter, generates stable clones that express the target protein. Cultivation employing the synthetic medium SYN6(10) with controlled parameters (e.g. temperature, pH, feeding strategy, and aeration) produces 0.15 µg/mL of HPV type 52 L1 protein, suggesting a possibility for scaling up to a higher production level. Conclusion: The scale-up production of HPV type 52 L1 protein using Hansenula polymorpha expression system described in this study provides an opportunity for an economical manufacturing platform for the development of the HPV vaccine. © 2024

Author Keywords

DASGIP® parallel bioreactor system; Hansenula polymorpha; HPV type 52 L1 protein; Synthetic medium

Index Keywords

Funding Details

National Research and Innovation Agency/Badan; Lembaga Pengelola Dana Pendidikan, LPDP, (B-1738/II.7.5/FR/11/2022); Lembaga Pengelola Dana Pendidikan, LPDP

Funding Text

This study was supported by National Research and Innovation Agency/Badan Riset dan Inovasi Nasional (BRIN) and Lembaga Pengelola Dana Pendidikan (LPDP) under scheme of Program Riset dan Inovasi untuk Indonesia Maju (RIIM) with the contract number: No. B-1738/II.7.5/FR/11/2022.

Publisher

Elsevier B.V.

Issn

1687157X

Isbn

Pubmed Id

Document Type

Article

Sjr Best Quartile

Sjr

560

Sjr Rank

9245