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Publikasi Scopus 2024 per tanggal 31 Mei 2024 (409 artikel)
Publikasi Scopus 2024 per tanggal 31 Mei 2024 (409 artikel)
Khairullah, Aswin Rafif (57212026505); Kurniawan, Shendy Canadya (57863649900); Sudjarwo, Sri Agus (6603340449); Effendi, Mustofa Helmi (57191326453); Widodo, Agus (58835559800); Moses, Ikechukwu Benjamin (57211283734); Hasib, Abdullah (57219295438); Zahra, Reichan Lisa A. (58930245700); Gelolodo, Maria Aega (57741298000); Kurniawati, Dyah Ayu (57224130404); Riwu, Katty Hendriana Priscilia (57218829426); Silaen, Otto Sahat Martua (57219408428); Afnani, Daniah Ashri (58055714000); Ramandinianto,
Kinship analysis of mecA gene of methicillin-resistant Staphylococcus aureus isolated from milk and risk factors from the farmers in Blitar, Indonesia
2024
Veterinary World
17
1
216
225
9
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85187344738&doi=10.14202%2fvetworld.2024.216-225&partnerID=40&md5=b97db560991ef8524eb7d71dff586f6d
Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Master Program of Animal Sciences, Department of Animal Sciences Specialisation in Molecule Cell and Organ Functioning, Wageningen University and Research, Wageningen, 6708 PB, Netherlands; Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Department of Health, Faculty of Vocational Studies, Universitas Airlangga, Jl. Dharmawangsa Dalam Selatan No. 28-30, Kampus B Airlangga, East Java, Surabaya, 60115, Indonesia; Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, 480211, Nigeria; School of Agriculture and Food Sustainability, The University of Queensland, Queensland, Gatton, 4343, QLD, Australia; Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Jl. Adisucipto Penfui, East Nusa Tenggara, Kupang, 85001, Indonesia; Indonesia Research Center for Veterinary Science, Jl. RE Martadinata No. 30, West Java, Bogor, 16114, Indonesia; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Jl. Pemuda No. 59A, Dasan Agung Baru, West Nusa Tenggara, Mataram, 83125, Indonesia; Doctoral Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6 Senen, Jakarta, 10430, Indonesia; Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Jl. Pemuda No. 59A, Dasan Agung Baru, West Nusa Tenggara, Mataram, 83125, Indonesia; Lingkar Satwa Animal Care Clinic, Jl. Sumatera No. 31L, Gubeng, East Java, Surabaya, 60281, Indonesia
Background and Aim: There are numerous reports of subclinical mastitis cases in Blitar, which is consistent with the region's high milk production and dairy cattle population. Staphylococcus aureus, which is often the cause of mastitis cases, is widely known because of its multidrug-resistant properties and resistance to β-lactam antibiotic class, especially the methicillin-resistant S. aureus (MRSA) strains. This study aimed to molecular detection and sequence analysis of the mecA gene in milk and farmer's hand swabs to show that dairy cattle are reservoirs of MRSA strains. Materials and Methods: A total of 113 milk samples and 39 farmers' hand swab samples were collected from a dairy farm for the isolation of S. aureus using Mannitol salt agar. The recovered isolates were further characterized using standard microbiological techniques. Isolates confirmed as S. aureus were tested for sensitivity to antibiotics. Oxacillin Resistance Screening Agar Base testing was used to confirm the presence of MRSA, whereas the mecA gene was detected by polymerase chain reaction and sequencing. Results: A total of 101 samples were confirmed to be S. aureus. There were 2 S. aureus isolates that were multidrug-resistant and 14 S. aureus isolates that were MRSA. The mecA gene was detected in 4/14 (28.6%) phenotypically identified MRSA isolates. Kinship analysis showed identical results between mecA from milk and farmers' hand swabs. No visible nucleotide variation was observed in the two mecA sequences of isolates from Blitar, East Java. Conclusion: The spread of MRSA is a serious problem because the risk of zoonotic transmission can occur not only to people who are close to livestock in the workplace, such as dairy farm workers but also to the wider community through the food chain. © 2024 Veterinary World. All rights reserved.
cefoxitin; erythromycin; gentamicin; tetracycline; agricultural worker; antibiotic resistance; antibiotic sensitivity; antimicrobial activity; Article; bacterial gene; bacterial growth; bacterium isolate; controlled study; disk diffusion; DNA extraction; Escherichia coli; human; methicillin resistant Staphylococcus aureus; milk; nonhuman; phylogenetic tree; polymerase chain reaction; risk factor; sequence analysis
Universitas Airlangga Tahun, (LPPM/ PT.01.03/2023); Skema Penelitian Unggulan Airlangga; PUA
This study was supported in part by the Skema Penelitian Unggulan Airlangga (PUA) Universitas Airlangga Tahun 2023, Number: 1710/UN3.LPPM/ PT.01.03/2023.
Veterinary World
09728988
Article
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