Publikasi Scopus 2010 s/d 2022

Authors_(Last_Name)

Sihombing U.H.M., Andrijono A., Purwoto G., Gandamihardja S., Harahap A.R., Rustamadji P., Kekalih A., Widyawati R., Fuady D.R.

Author S Id

57741696900;36704391000;14720170400;54880603300;6507325543;55321572200;55633562200;57212476754;57198896312;

Title

CD44+/CD24- Expression as predictors of ovarian cancer chemoresistance: immunohistochemistry and flow cytometry study

Year

2022

Source Title

Journal of the Egyptian National Cancer Institute

Volume

Issue

Art No

Page Start

Page End

Page Count

Cited By

Link

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140345139&doi=10.1186%2fs43046-022-00143-2&partnerID=40&md5=0304827359ed0879ee36bb32af566784

Affiliations

Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Tarakan Hospital, Jakarta, Indonesia; Doctoral Program in Medical Sciences Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Hasan Sadikin Hospital, Universitas Padjadjaran, Bandung, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia — Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Department of Anatomic Pathology, Faculty of Medicine Universitas Indonesia — Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Occupational Medicine Division, Community Medicine Department, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Department of Anatomic Pathology, Budhi Asih Hospital, Jakarta, Indonesia; Department of Obstetrics and Gynecology, Karawang General Public Hospital, Karawang, Indonesia

Authors With Affiliations

Sihombing, U.H.M., Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Tarakan Hospital, Jakarta, Indonesia, Doctoral Program in Medical Sciences Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Andrijono, A., Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Purwoto, G., Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Gandamihardja, S., Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Hasan Sadikin Hospital, Universitas Padjadjaran, Bandung, Indonesia; Harahap, A.R., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia — Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Rustamadji, P., Department of Anatomic Pathology, Faculty of Medicine Universitas Indonesia — Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Kekalih, A., Occupational Medicine Division, Community Medicine Department, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Widyawati, R., Department of Anatomic Pathology, Faculty of Medicine Universitas Indonesia — Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Department of Anatomic Pathology, Budhi Asih Hospital, Jakarta, Indonesia; Fuady, D.R., Gynecologic-Oncology Division, Department of Obstetrics and Gynecology, Tarakan Hospital, Jakarta, Indonesia, Department of Obstetrics and Gynecology, Karawang General Public Hospital, Karawang, Indonesia

Abstract

Background: The conventional standard treatment for ovarian cancer is not very effective, and the disease is fatal for women. Cancer Stem Cells (CSCs) that express CD44+/CD24- can contribute to chemoresistance and a poor prognosis. We seek to investigate the expression of CSCs (CD44+/CD24-) in ovarian cancer and their predictive significance. Methods: The ambispective cohort was performed on 64 patients (32 patients in each group) at four hospitals (Cipto Mangunkusumo, Tarakan, Fatmawati, and Dharmais Hospital). Debulking surgery was performed on the patients, followed by histopathological analysis. The patients had six rounds of chemotherapy and were under monitoring for six months. The therapeutic responses were evaluated using the RECIST criteria (Response Criteria in Solid Tumors) and categorized as chemoresistant or chemosensitive. Using immunohistochemistry, we directly assess the CSCs from ovarian cancer tissue and using flow cytometry to assess the CSCs from the blood. Results: High CSCs expression and ovarian cancer chemoresistance were significantly related in both trials (p 0.05). A better outcome was obtained using CD44+/CD24- immunohistochemistry. Conclusions: We conclude that there is a substantial association between high CSCs expression and chemoresistance in ovarian cancer and that CSCs immunohistochemistry has a higher predictive value. © 2022, The Author(s).

Author Keywords

CD44+/CD24-; Chemoresistance; Flow cytometry; Immunohistochemistry; Ovarian Cancer

Index Keywords

antineoplastic agent; CA 125 antigen; CD24 antigen; Hermes antigen; CD24 antigen; CD24 protein, human; CD44 protein, human; hyaluronic acid binding protein; tumor marker; adult; antigen blood level; antigen expression; Article; ascites; blood sampling; cancer chemotherapy; cancer resistance; cancer size; cancer staging; cancer stem cell; cell differentiation; chemosensitivity; cohort analysis; controlled study; cytoreductive surgery; diagnostic accuracy; female; flow cytometry; histopathology; human; human tissue; immunohistochemistry; lymph node metastasis; major clinical study; middle aged; multicenter study; multiple cycle treatment; ovary carcinoma; patient monitoring; prediction; predictive value; response evaluation criteria in solid tumors; sensitivity and specificity; treatment out

Funding Details

Funding Text 1

We would thank Cipto Mangunkusumo, Tarakan Hospital, Dharmais hospital, and Fatmawati Hospital for their support in this research. We would like to acknowledge Christine Pamphila, M.D., Rasendah, M.D., Kristabella Gianina, M.D., for their contribution to data analysis and data collection.

Funding Text 2

Funding Text 3

Publisher

Springer Science and Business Media Deutschland GmbH

Issn

11100362

Isbn

Pubmed Id

36274112

Document Type

Article

Sjr Best Quartile

Sjr

336

Sjr Rank

13709