No records
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814 |
Mirtha L.T., Siagian M., Tamin T.Z., Radi B., Soemarko D.S., Prijanti A.R., Irfanuddin M., Pelana R., Mansyur M. |
57193201450;6505956355;23476060900;6603494019;57192889490;57190863386;57223257669;57202117393;37085506800; |
The formula for calculating the predicted value of cardiorespiratory endurance using a foot rest-based cardiorespiratory exercise device |
2021 |
Science Progress |
104 |
2 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105102326&doi=10.1177%2f00368504211010637&partnerID=40&md5=2c4408c0d2414931797e6357c72a1d59 |
Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Departement of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Departement of Medical Rehabilitation, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Departement of Biochemistry and Biology Molecular, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Departement of Physiology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia; Faculty of Sports Science, University State of Jakarta, Jakarta, Indonesia |
Mirtha, L.T., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Siagian, M., Departement of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Tamin, T.Z., Departement of Medical Rehabilitation, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Radi, B., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Soemarko, D.S., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Prijanti, A.R., Departement of Biochemistry and Biology Molecular, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Irfanuddin, M., Departement of Physiology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia; Pelana, R., Faculty of Sports Science, University State of Jakarta, Jakarta, Indonesia; Mansyur, M., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
This study developed a formula for calculating the predicted VO2 max value using a prototype model of foot-based cardiorespiratory exercise. Forty sedentary workers (20 men and 20 women) were enrolled via consecutive sampling. They underwent direct measurement of VO2 max using spiroergometry as the gold standard; the predicted VO2 max value was calculated using a prototype model of foot-based cardiorespiratory exercise, which was performed on consecutive days. Multivariate linear regression analysis was used to formulate the equation for the predicted VO2 max value by including potential contributing variables: gender, body height, body weight and heart rate. Bland–Altman test was used for assessing the agreement level for the predicted VO2 max value. The equation for the predicted VO2 max value was formulated as 3.2 + 0.15 optimal exercise heart rate −5.5 sexes (0 for men, 1 for women). The agreement level for the formula was acceptable in all measurement result ranges. The formula developed in this study can be used to measure the predicted VO2 max value with an acceptable agreement level. © The Author(s) 2021. |
Cardiorespiratory endurance; level of agreement; physical exercise; physical fitness; predicted VO2 max; sedentary work; sitting workers; VO2 max |
adult; article; body height; body weight; clinical article; endurance; exercise; female; foot; gender; gold standard; heart rate; human; human experiment; human tissue; linear regression analysis; male; sitting; worker |
SAGE Publications Ltd |
00368504 |
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33913389 |
Article |
Q2 |
310 |
14499 |
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817 |
Kusumawardani A., Yanfaunnas A.M., Supandi D.P., Inggita R.A.M., Andayani N.G.A.A.P.T., Louisa M., Soetikno V., Burhan E. |
57223083920;57223095441;57222624055;57223087394;57223088955;41461551400;36769252100;36058554600; |
The use of Urinary Lipoarabinomannan (LAM) as a Rapid Diagnostic Test for Adult Pulmonary Tuberculosis in HIV-Positive Patients: An Evidence-based Case Report |
2021 |
Journal of International Dental and Medical Research |
14 |
1 |
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461 |
466 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104790784&partnerID=40&md5=012c79128a2fc69230627fe766651487 |
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine Universitas Indonesia/Persahabatan Hospital, Jakarta, Indonesia |
Kusumawardani, A., Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Yanfaunnas, A.M., Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Supandi, D.P., Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Inggita, R.A.M., Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Andayani, N.G.A.A.P.T., Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Louisa, M., Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Soetikno, V., Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Burhan, E., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine Universitas Indonesia/Persahabatan Hospital, Jakarta, Indonesia |
Tuberculosis is a major health concerns around the world as it is causing morbidity and mortality, especially in HIV infected patients. Detection of lipoarabinomannan (LAM) on urine is a non-sputum-based diagnostic test for TB that could accelerate TB case detection in HIV-positive patients. This evidence-based case report was aimed to evaluate the diagnostic value of urinary Lipoarabinomannan (LAM) test in diagnosing pulmonary tuberculosis in HIV-positive patients as compared to sputum culture. Structured literature searching was done on cross-sectional or systematic reviews of diagnostic studies of lipoarabinomannan versus sputum culture in adult HIV-positive patients suspected with pulmonary TB. There were 9 articles that meet the eligibility criteria; 1 systematic review and 8 cross-sectional studies. Two out of nine articles were deemed as not valid due to not using sputum on all samples and the index test and reference test were not carried out on all samples. There were contradictory results regarding the sensitivity, positive predictive values and negative predictive values, meanwhile the specificity showed good results. We conclude that urinary LAM can be used to diagnose pulmonary TB in HIV-positive patients due to its high specificity. However, it could not be used as a stand-alone test, due to its suboptimal sensitivity. © 2021 |
Sputum culture; TB-HIV; tuberculosis; urinary lipoarabinomannan |
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University of Dicle |
1309100X |
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Article |
Q3 |
259 |
16312 |
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818 |
Sahar N., Birowo P., Kusmardi, Kristianty D., Rahmaningrum K., Miranda A.V., Rasyad A., Prasasty V.D. |
57212464367;6504153311;56966625300;57223024424;57223023016;57222664687;57223027205;56019989700; |
Mucin-1 expression in endometrial tissue of Macaca nemestrina during mid-luteal phase after controlled-ovarian hyperstimulation |
2021 |
Biodiversitas |
22 |
4 |
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1927 |
1933 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104548199&doi=10.13057%2fbiodiv%2fd220440&partnerID=40&md5=436be5fa1fbe0e2157ca68a386731018 |
Department of Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Departement Urology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Faculty of Biotechnology, Universita Katolik Atma Jaya Indonesia, Jl. Jend. Sudirman No. 51, Jakarta Selatan, Jakarta, 12930, Indonesia |
Sahar, N., Department of Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Birowo, P., Departement Urology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Kusmardi, Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Kristianty, D., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Rahmaningrum, K., Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Miranda, A.V., Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Rasyad, A., Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, Jakarta, 10430, Indonesia; Prasasty, V.D., Faculty of Biotechnology, Universita Katolik Atma Jaya Indonesia, Jl. Jend. Sudirman No. 51, Jakarta Selatan, Jakarta, 12930, Indonesia |
Endometrial receptivity is one of the factors for successful implantation in pregnancy. Controlled ovarian hyperstimulation (COH) is a required step of in vitro fertilization (IVF), one of the standard procedures to overcome infertility. Exogenous gonadotropin hormones from COH provoke the secretion of estrogen and progesterone from the ovaries in higher amounts. The supraphysiological environment could impact the endometrial receptivity of the implantation process. Mucin-1 (MUC1) can be used as a marker to indicate alterations in the endometrial tissue. Therefore, this study aimed to investigate the alteration of mucin-1 expression in endometrial tissue of Macaca nemestrina after COH protocol. This study used endometrium tissue of M. nemestrina embedded with paraffin as tissue blocks. The subjects were 15 female macaques in reproductive age (8-10 years old) with a history of producing offspring. These macaques were classified into four groups based on the COH protocols, which consist of administering gonadotropin-releasing hormone (GnRH) agonist and recombinant FSH (r-FSH) with dosages of 30 IU, 50 IU, 70 IU (intervention groups), and no r-FSH (control group). The stimulations were administered for 14 days during the mid-luteal phase. Moreover, tissue staining was done by using immunohistochemistry. Mucin-1 expression was analyzed manually for glandular and luminal compartments and automatically for the stromal compartment by Red Green Blue (RGB) measure plugin as a color image from ImageJ software. The expression of mucin-1 was counted semi-quantitatively as H-score. Mucin-1 expression differences in endometrial tissue were assessed by one-way ANOVA with a significant difference found in the glandular part and insignificant results found in luminal and stromal parts. Pearson correlation test was also performed to determine the relationship of steroidal hormones and mucin-1 expression in glandular and luminal compartments. Both correlations were statistically insignificant. However, a positive correlation was found with the glandular compartment and a negative correlation with luminal compartment. As the subject species, M. nemestrina is limited for research utilization in our country, small number of subjects becomes a limitation of this study. Further studies need to be conducted to deepen the understanding of the regulation of mucin-1 expression, particularly by the altered concentrations of steroidal hormones as a consequence of COH. © 2021, Society for Indonesian Biodiversity. All rights reserved. |
Controlled ovarian hyperstimulation; Endometrial receptivity; In vitro fertilization; Mucin-1 |
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Society for Indonesian Biodiversity |
1412033X |
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Article |
Q3 |
257 |
16376 |
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819 |
Putri A.S., Soedarsono N., Nehemia B., Atmadja D.S., Ubelaker D.H. |
57223021361;14049161500;57204102056;6507824506;7003416830; |
Age estimation of individuals aged 5–23 years based on dental development of the Indonesian population |
2021 |
Forensic Sciences Research |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104502186&doi=10.1080%2f20961790.2021.1886648&partnerID=40&md5=b3c0a614893fe57774dae608436d737a |
Department of Oral Biology, Division of Forensic Odontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Department of Dento-Maxillofacial Radiology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Department of Forensic Medicine and Medico-legal, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anthropology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States |
Putri, A.S., Department of Oral Biology, Division of Forensic Odontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Soedarsono, N., Department of Oral Biology, Division of Forensic Odontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Nehemia, B., Department of Dento-Maxillofacial Radiology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Atmadja, D.S., Department of Forensic Medicine and Medico-legal, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Ubelaker, D.H., Department of Anthropology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States |
Dental development can be used to estimate age for forensic purposes. However, most of the currently available methods are less reliable for the Indonesian population due to population variability. This study presents a new method and evaluates other methods that utilize dental development to estimate the age of Indonesian people. Panoramic radiographs of 304 young Indonesian people aged 5–23 years old were analysed for deciduous tooth root resorption, permanent tooth calcification, and eruption. The extent of tooth root resorption was determined based on AlQahtani’s modified Moorrees et al. method. Tooth calcification was classified based on a modified Demirjian et al. method. Tooth eruption was evaluated based on AlQahtani’s modified Bengston system. The sequence of tooth root resorption, and permanent tooth calcification and eruption were grouped into 19 age categories (from 5–23 years old) in an atlas. The differences between males and females, between maxillary and mandibular teeth, and between right and left teeth were also analysed. There were minimal significant differences of tooth development between males and females, and between the right and left teeth (P > 0.05), while the maxillary and mandibular dental development was significantly different (P < 0.05). The newly developed atlas showed the development of the right side of maxillary and mandibular tooth of combined sex of Indonesian population. Another 34 panoramic radiographs of known-age and sex individuals from Indonesia were assessed using the newly developed Atlas of Dental Development in the Indonesian Population, Ubelaker’s Dental Development Chart, The London Atlas of Human Tooth Development and Eruption by AlQahtani, and the Age Estimation Guide-Modern Australia population by Blenkin-Taylor. Accuracy was assessed by comparing estimated age to actual chronological age using the Bland-Altmand test. Results show that the smallest range of error was found in the Atlas of Dental Development in the Indonesian Population (−0.969 to 1.210 years), followed by The London Atlas of Human Tooth Development and Eruption by AlQahtani (−2.013 to 1.990 years), the Age Estimation Guide-Modern Australia population by Blenkin-Taylor (−2.495 to 2.598 years), the Dental Development Chart by Ubelaker (−2.960 to 3.289 years). These findings show that the Atlas of Dental Development constructed in this study performs better than the other three methods and presents greater accuracy of age estimation in the Indonesian population. Key points •Dental development such as deciduous tooth root resorption, permanent tooth calcification, and tooth eruption can be used to estimate age for forensic purposes. •The development of the teeth are influenced by genetic, ethnicity, and sex, therefore an age estimation method must be constructed based on the same population. •There were minimal significant differences in tooth development between male and female, and between right and left teeth, but there was significant difference between maxillary and mandibular teeth. •The Atlas of Dental Development in the Indonesian Population constructed in this study allowed more accurate age estimation of the Indonesian sample than the other methods tested. Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.1886648. © 2021 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science. |
age estimation; forensic odontology; Forensic sciences; root resorption; tooth calcification; tooth eruption |
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Taylor and Francis Ltd. |
20961790 |
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Article |
Q1 |
729 |
6779 |
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820 |
Rini I.S., Gunardi A.J., Yashinta, Kevin J., Marsaulina R.P., Aryandono T., Dachlan I., Dwiprahasto I. |
57189690245;57215216664;57223006688;57223012633;57220026579;23495038400;57210391374;6506398126; |
Quality of Life in Palliative Post-mastectomy Reconstruction: Keystone versus Rotational Flap |
2021 |
Plastic and Reconstructive Surgery - Global Open |
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e3457 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104488039&doi=10.1097%2fGOX.0000000000003457&partnerID=40&md5=4e95d37965469db84b820afb28853499 |
Department of Plastic Surgery, Dharmais Cancer Hospital, Jalan Letjen Jend. S. Parman No. 84-86, West Jakarta, DKI Jakarta, 11420, Indonesia; Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, Indonesia; Faculty of Medicine, Universitas Kristen Indonesia, Central Jakarta, DKI Jakarta, Indonesia; Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia |
Rini, I.S., Department of Plastic Surgery, Dharmais Cancer Hospital, Jalan Letjen Jend. S. Parman No. 84-86, West Jakarta, DKI Jakarta, 11420, Indonesia; Gunardi, A.J., Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, Indonesia; Yashinta, Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, Indonesia; Kevin, J., Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, Indonesia; Marsaulina, R.P., Faculty of Medicine, Universitas Kristen Indonesia, Central Jakarta, DKI Jakarta, Indonesia; Aryandono, T., Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia; Dachlan, I., Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia; Dwiprahasto, I., Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia |
Background: Late stage breast cancer presents with malignant wound causing skin infiltration, pain, bleeding, and malodour, which affect quality of life (QoL). Palliative mastectomy aims to eliminate wound symptoms and requires prolonged wound care to improve QoL. This study aimed to prospectively investigate QoL differences in 2 alternative reconstructive methods: keystone flap and rotational flap. Methods: Twenty-four late stage breast cancer patients with symptoms of cancer wounds were included in this study. They were divided into 2 groups: keystone flap and rotational flap. Each patient's QoL was evaluated using EORTC QLQ-C30 and QLQ-BR23 before and 3 weeks after surgery. Results: Global health post-surgery was significantly improved compared with pre-surgery in all patients (P < 0.001), across both the keystone (P = 0.018) and rotational groups (P = 0.007). Breast symptoms post-surgery were also improved compared with pre-surgery in all patients (P = 0.035). However, when analyzed per group, breast symptoms were only improved significantly in the keystone group (P = 0.013) but not in the rotational group (P = 0.575). When compared between 2 groups, future perspective post-surgery in the keystone group [100 (0-100)] was better than the rotational group [66.7 (0-100)], (P = 0.020). Conclusions: Reconstructive surgery after mastectomy improves QoL in late stage breast cancer patients. The keystone flap is superior to the rotational flap in improving global health and breast symptoms. © 2021 Georg Thieme Verlag. All rights reserved. |
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Lippincott Williams and Wilkins |
21697574 |
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Article |
Q2 |
759 |
6437 |
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821 |
Wanandi S.I., Arumsari S., Afitriyansyah E., Syahrani R.A., Dewantara I.R., Nurachman L.A., Amin I.F., Haryono P.D., Budiman K., Sugiharta A.J., Remedika A.A., Taufikulhakim F.H., Iswanti F.C., Lee J.Y., Banerjee D. |
36099320700;57193202336;57223005545;57204103147;57223018344;57222663184;57188973524;57223016376;57223010670;57223013711;57223000993;57222619312;57192920851;57223019852;7202633380; |
Elevated extracellular co2 level affects the adaptive transcriptional response and survival of human peripheral blood mononuclear cells toward hypoxia and oxidative stress |
2021 |
Medical Journal of Indonesia |
30 |
1 |
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5 |
12 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104485331&doi=10.13181%2fmji.oa.203810&partnerID=40&md5=bf8dedf67718ab440cf4f665b5af2f83 |
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Molecular Biology and Proteomics Core Facilities, Indonesian Medical and Education Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Biomedical Science Master Program, Rutgers-Robert Wood Johnson Medical SchoolNJ, United States; USAID Research Innovation Fellowship 2017, Washington, DC, United States; Department of Pharmacology, Rutgers-Robert Wood Johnson Medical SchoolNJ, United States |
Wanandi, S.I., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Molecular Biology and Proteomics Core Facilities, Indonesian Medical and Education Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Arumsari, S., Molecular Biology and Proteomics Core Facilities, Indonesian Medical and Education Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Afitriyansyah, E., Molecular Biology and Proteomics Core Facilities, Indonesian Medical and Education Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Syahrani, R.A., Molecular Biology and Proteomics Core Facilities, Indonesian Medical and Education Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Dewantara, I.R., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Nurachman, L.A., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Amin, I.F., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Haryono, P.D., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Budiman, K., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sugiharta, A.J., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Remedika, A.A., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Taufikulhakim, F.H., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Iswanti, F.C., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Molecular Biology and Proteomics Core Facilities, Indonesian Medical and Education Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Lee, J.Y., Biomedical Science Master Program, Rutgers-Robert Wood Johnson Medical SchoolNJ, United States, USAID Research Innovation Fellowship 2017, Washington, DC, United States; Banerjee, D., Department of Pharmacology, Rutgers-Robert Wood Johnson Medical SchoolNJ, United States |
BACKGROUND High carbon dioxide (CO2 ) level from indoor environments, such as classrooms and offices, might cause sick building syndrome. Excessive indoor CO2 level increases CO2 level in the blood, and over-accumulation of CO2 induces an adaptive response that requires modulation of gene expression. This study aimed to investigate the adaptive transcriptional response toward hypoxia and oxidative stress in human peripheral blood mononuclear cells (PBMCs) exposed to elevated CO2 level in vitro and its association with cell viability. METHODS PBMCs were treated in 5% CO2 and 15% CO2, representatives a high COâ‚‚ level condition for 24 and 48 hours. Extracellular pH (pHe) was measured with a pH meter. The levels of reactive oxygen species were determined by measuring superoxide and hydrogen peroxide with dihydroethidium and dichlorofluorescin-diacetate assay. The mRNA expression levels of hypoxia-inducible factor (HIF)-1α, HIF-2α, nuclear factor (NF)-κB, and manganese superoxide dismutase (MnSOD) were analyzed using a real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Cell survival was determined by measuring cell viability. RESULTS pHe increased in 24 hours after 15% COâ‚‚ treatment, and then decreased in 48 hours. Superoxide and hydrogen peroxide levels increased after the 24-and 48-hour of high COâ‚‚ level condition. The expression levels of NF-κB, MnSOD, HIF-1α, and HIF-2α decreased in 24 hours and increased in 48 hours. The increased antioxidant mRNA expression in 48 hours showed that the PBMCs were responsive under high CO2 conditions. Elevated CO2 suppressed cell viability significantly in 48 hours. CONCLUSIONS After 48 hours of high COâ‚‚ level condition, PBMCs showed an upregulation in genes related to hypoxia and oxidative stress to overcome the effects of CO2 elevation. © 2021 Authors. |
Elevated CO2; Extracellular pH; Oxidative stress; PBMC; Reactive oxygen species |
carbon dioxide; hydroethidine; hypoxia inducible factor 1alpha; hypoxia inducible factor 2alpha; immunoglobulin enhancer binding protein; manganese superoxide dismutase; messenger RNA; reactive oxygen metabolite; adult; Article; cell survival; cell viability; controlled study; extracellular space; gene expression; genetic transcription; human; human cell; human experiment; hypoxia; male; mRNA expression level; normal human; oxidative stress; peripheral blood mononuclear cell; pH; real time reverse transcription polymerase chain reaction; upregulation; young adult |
Faculty of Medicine, Universitas Indonesia |
08531773 |
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Article |
Q4 |
164 |
21905 |
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822 |
Ramlan A.A.W., Ratnaningrum A., Marsaban A.H.M. |
57195941841;57223018172;57195383833; |
A randomized controlled trial of infraorbital block using ketamine 1% for intra-and postoperative analgesia in children for ambulatory cleft lip correction |
2021 |
Medical Journal of Indonesia |
30 |
1 |
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33 |
38 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104479826&doi=10.13181%2fmji.oa.204395&partnerID=40&md5=c08932eaa2373ffcc535ff0eadd8e55b |
Department of Anesthesiology and Intensive Care, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anesthesiology, Fatmawati General Hospital, Jakarta, Indonesia |
Ramlan, A.A.W., Department of Anesthesiology and Intensive Care, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Ratnaningrum, A., Department of Anesthesiology, Fatmawati General Hospital, Jakarta, Indonesia; Marsaban, A.H.M., Department of Anesthesiology and Intensive Care, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
BACKGROUND In Indonesia, cleft lip correction surgery is often done as a social program in remote areas with limited resources. This study aimed to assess the effectiveness of ketamine, a cheaper and more accessible alternative, as a local analgesia in infraorbital block and to determine the possibility of ketamine as an alternative local analgesic drug for intraoperative and postoperative periods. METHODS This was a randomized controlled trial in children aged 2 months to 5 years who underwent cleft lip correction surgery at Cipto Mangunkusumo Hospital in 2016. Subjects were randomly divided into two groups: ketamine and bupivacaine. Standard general anesthesia with endotracheal intubation was performed in each group. Bilateral intraoral infraorbital block was performed using ketamine 1% 0.5 ml or bupivacaine 0.25% 0.5 ml. Postoperative evaluation includes pain scores based on the face, leg, activity, cry, and consolability (FLACC) scale and analgesic duration. RESULTS A total of 36 subjects were enrolled in this study, with 18 in each group. Both groups received the same total amount of fentanyl addition intraoperatively (p = 1). The postoperative FLACC pain scale scores between the two groups were not different, with p>0.05 in every measurement. The mean duration of postoperative analgesia in the ketamine group was longer than the bupivacaine group (15–13.49 hours, p = 0.031). CONCLUSIONS Infraorbital block with 1% ketamine 0.5 mg/kg was similarly effective for intraoperative and postoperative analgesia but had a longer duration than that with 0.25% bupivacaine 0.5 ml in ambulatory cleft lip correction. © 2021 Authors. |
Cleft lip; Ketamine; Postoperative pain |
bupivacaine; epinephrine; fentanyl; ketamine; paracetamol; sevoflurane; Article; breathing rate; child; cleft lip; cleft palate; clinical article; controlled study; endotracheal intubation; female; FLACC scale; follow up; general anesthesia; hemodynamics; human; infant; infraorbital nerve; infraorbital nerve block; intraoperative period; male; nerve block; pain assessment; postoperative analgesia; postoperative pain; pulse rate; randomized controlled trial; single blind procedure; telephone interview; visual analog scale |
Faculty of Medicine, Universitas Indonesia |
08531773 |
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Article |
Q4 |
164 |
21905 |
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823 |
Sarika D.T., Louisa M., Rozaliyani A., Evelina, Susiyanti M. |
57223018187;41461551400;57203065912;57223007552;19640377300; |
Efficacy of adjuvant intrastromal and combination of intrastromal and intracameral voriconazole in aspergillus fumigatus-induced moderate fungal keratitis in rabbits |
2021 |
Medical Journal of Indonesia |
30 |
1 |
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13 |
19 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104464172&doi=10.13181%2fmji.oa.203726&partnerID=40&md5=6021b6878c47eb4ccde69bba65f51abb |
Department of Ophthalmology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Sarika, D.T., Department of Ophthalmology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Louisa, M., Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Rozaliyani, A., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Evelina, Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Susiyanti, M., Department of Ophthalmology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
BACKGROUND There is no in vivo evidence for the effectiveness of adjuvant intrastromal and combination of intrastromal and intracameral voriconazole (VCZ) for treating Aspergillus fumigatus keratitis. This study aimed to compare the efficacy of both agents against it. METHODS A randomized, masked, controlled experimental study was conducted on 11 albino New Zealand white rabbits in which moderate fungal keratitis was induced by inoculating spores of A. fumigatus to the cornea. The rabbits were allocated into 3 groups: 50 μg/0.1 ml intrastromal VCZ injection, 50 μg/0.1 ml intrastromal and intracameral VCZ injections, and topical VCZ (control). The treatment was given 5 days after inoculation. Epithelial defect, infiltrate size, corneal ulcer depth, and hypopyon were evaluated clinically. Histopathological and mycological examinations were also done 14 days after treatment. RESULTS All rabbits in the adjuvant treatment groups demonstrated a tendency of a better clinical response with decreasing size of epithelial defect (p = 0.679) and infiltrate (p = 0.755) than in the control group. Direct microscopy, corneal culture, and chop corneal tissue culture were still positive in most of the rabbits from all groups. Histopathological examination showed an increase of inflammatory cells after treatment in all groups, especially in rabbits which were inoculated with A. fumigatus spores in both eyes. CONCLUSIONS An adjuvant combination of intrastromal and intracameral VCZ showed a tendency of better clinical response for A. fumigatus-induced moderate fungal keratitis in rabbits. © 2021 Authors. |
Aspergillus fumigatus; Fungal keratitis; Intrastromal; Voriconazole |
eye drops; ketamine; pentobarbital; potassium hydroxide; prednisone acetate; tetracaine; voriconazole; xylazine; animal experiment; animal model; animal tissue; Article; Aspergillus fumigatus; controlled study; cornea injury; cornea ulcer; corneal scraping; disk diffusion; drug efficacy; follow up; fungal examination; histopathology; hypopyon; inflammation; intrastromal drug administration; keratectomy; keratomycosis; Leporidae; male; nonhuman; treatment response |
Faculty of Medicine, Universitas Indonesia |
08531773 |
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Article |
Q4 |
164 |
21905 |
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824 |
Agung N.P., Nadhif M.H., Irdam G.A., Mochtar C.A. |
57222963984;57189057498;57194729795;6506558321; |
The Role of 3D-Printed Phantoms and Devices for Organ-specified Appliances in Urology |
2021 |
International Journal of Bioprinting |
7 |
2 |
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1 |
14 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104261313&doi=10.18063%2fIJB.V7I2.333&partnerID=40&md5=0bbeefb808ac4fbf51023802ccfa25db |
Department of Urology, Universitas Indonesia, Faculty of Medicine/Ciptomangunkusumo Central Hospital, Jakarta, Indonesia; Department of Medical Physics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Medical Technology Cluster, Indonesian Medical Education and Research Institute, Jakarta, Indonesia |
Agung, N.P., Department of Urology, Universitas Indonesia, Faculty of Medicine/Ciptomangunkusumo Central Hospital, Jakarta, Indonesia; Nadhif, M.H., Department of Medical Physics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Medical Technology Cluster, Indonesian Medical Education and Research Institute, Jakarta, Indonesia; Irdam, G.A., Department of Urology, Universitas Indonesia, Faculty of Medicine/Ciptomangunkusumo Central Hospital, Jakarta, Indonesia; Mochtar, C.A., Department of Urology, Universitas Indonesia, Faculty of Medicine/Ciptomangunkusumo Central Hospital, Jakarta, Indonesia |
Urology is one of the fields that are always at the frontline of bringing scientific advancements into clinical practice, including 3D printing (3DP). This study aims to discuss and presents the current role of 3D-printed phantoms and devices for organ-specified applications in urology. The discussion started with a literature search regarding the two mentioned topics within PubMed, Embase, Scopus, and EBSCOhost databases. 3D-printed urological organ phantoms are reported for providing residents new insight regarding anatomical characteristics of organs, either normal or diseased, in a tangible manner. Furthermore, 3D-printed organ phantoms also helped urologists to prepare a pre-surgical planning strategy with detailed anatomical models of the diseased organs. In some centers, 3DP technology also contributed to developing specified devices for disease management. To date, urologists have been benefitted by 3D-printed phantoms and devices in the education and disease management of organs of in the genitourinary system, including kidney, bladder, prostate, ureter, urethra, penis, and adrenal. It is safe to say that 3DP technology can bring remarkable changes to daily urological practices. © 2021 Agung, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
3D printing; Devices; Phantoms; Urology |
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Whioce Publishing Pte. Ltd. |
24248002 |
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Article |
Q1 |
1014 |
4306 |
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825 |
Wardoyo S., Pardede J.P.P., Asari H.A. |
57211106608;57222900416;57222898312; |
Patients’ characteristics following reoperation after modified blalock-taussig shunt (Mbts) in cardiac centre national general hospital cipto mangunkusumo from 2018-2020 |
2021 |
Bali Medical Journal |
10 |
1 |
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103 |
107 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104181375&doi=10.15562%2fbmj.v10i1.2124&partnerID=40&md5=71f0e48c1ff1cce6ec58341517f2fb6a |
Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia |
Wardoyo, S., Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; Pardede, J.P.P., Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; Asari, H.A., Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia |
Background: Modified Blalock-Taussig shunt (MBTS) is a common palliative procedure for congenital heart defect to connect the systemic to the pulmonary circulation via a synthetic shunt from a subclavian artery to a pulmonary artery. However, certain postoperative complications do exist, such as blockage. Early identification of modifiable risk factors is important to prompt early intervention for better outcomes. Material: This article is a descriptive narrative retrospective study. We gather data from the Cipto Mangunkusumo Hospital Cardiovascular Center medical report of 8 patients having undergone reoperation post-MBTS surgery as their first operation between January 2018 and October 2020. Data were analyzed using SPSS version 20 for Windows. Results: The patient’s age ranged from 3 months to 30 years old. Seven of them suffered from shunt occlusion complications, while the other suffered from the bleeding problem. The laboratory results showed that preoperative hemoglobin (18.9±4.1 g/dL vs. 16.90 (10.80-19.20) g/dL), hematocrit (58.8±12.40% vs. 42.9±14.30%), platelet counts (284.487±147.003 vs. 210.625±104.688) 103/uL, and oxygen saturation (86.00 (75.00-89.00) vs. 70.00±17.00) levels were higher than the pre-reoperative. The coagulation markers showed that PT value has increasing trend (1.10 (0.90-2.30) vs. 1.30±0.20) while aPTT (5.90 (2.90-6.30) vs. 2.30 (1.60-5.30)) seconds and activated clotting time (ACT) (205.00±86.00 vs. 165.00 (114.00-255.00)) showed the opposite. Conclusion: MBTS remains the first-choice bridging palliation surgery to increase the pulmonary blood flow in congenital heart defects. The cause of shunt failure remains inconclusive in this study. Other studies suggest that graft material choice, S/PA ratio, S/W ratio, operative approach, thrombus formation, and aPTT value contribute to shunt failure, thus needing a reoperation. © 2021, Sanglah General Hospital. All rights reserved. |
Characteristic; Modified Blalock-Taussig Shunt; Re-operation |
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Sanglah General Hospital |
20891180 |
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Article |
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