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680 |
Sandora N., Putra M.A., Nurhayati R.W., Suwarti, Nauli R., Kusuma T.R., Fitria N.A., Ardiansyah, Muttaqin C., Makdinata W., Alwi I. |
57204103434;57215605850;55748436600;57212462722;57212478305;57222897694;57148498800;57223036386;57226442135;57223405655;15055173800; |
Characterisation of the single-cell human cardiomyocytes taken from the excess heart tissue of the right ventricular outlet in congenital heart disease |
2021 |
Cell and Tissue Banking |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118214107&doi=10.1007%2fs10561-021-09970-4&partnerID=40&md5=2c4fad90958206777aa1ef5e9ea1e5ec |
Faculty of Medicine, Universitas Riau, Pekanbaru, 28293, Indonesia; Indonesia Medical Education and Research Institute, Jakarta, 10430, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Sandora, N., Faculty of Medicine, Universitas Riau, Pekanbaru, 28293, Indonesia, Indonesia Medical Education and Research Institute, Jakarta, 10430, Indonesia; Putra, M.A., Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Nurhayati, R.W., Indonesia Medical Education and Research Institute, Jakarta, 10430, Indonesia; Suwarti, Indonesia Medical Education and Research Institute, Jakarta, 10430, Indonesia; Nauli, R., Indonesia Medical Education and Research Institute, Jakarta, 10430, Indonesia; Kusuma, T.R., Indonesia Medical Education and Research Institute, Jakarta, 10430, Indonesia; Fitria, N.A., Indonesia Medical Education and Research Institute, Jakarta, 10430, Indonesia; Ardiansyah, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Muttaqin, C., Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Makdinata, W., Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Alwi, I., Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Cardiovascular disease is the second highest cause of death across the globe. Myocardial infarction is one of the heart diseases that cause permanent impairment of the heart wall leads to heart failure. Cellular therapy might give hope to regenerate the damaged myocardium. Single cells isolated from an excess heart tissue obtained from the correction of the right ventricular hypertrophy in patients with Tetralogy of Fallot for future heart study were investigated. Methods: Once resected, the heart tissues were transported at 37 °C, in Dulbecco's Modified Eagle's medium/ DMEM (4.5 g.L−1, antibiotic–antimycotic 3x, PRP10% (v/v)), to reach the lab within 30 min, weighted and grouped into less than 500 mg and more than 1000 mg (n = 4). Each sample was digested with 250 U.mL−1 Collagenase type V and 4U.mL−1 Proteinase XXIV in the MACS™ C-tube (Milltenyi, Germany), then dissociated using the MACS™ Octo Dissociator with Heater (Milltenyi, Germany) for 60 min at 37 °C. Results: All cells isolated were rod-shaped cells; viability was up to 90%. The cell density obtained from the 500 mg group were 4,867 ± 899 cells.mg−1 tissue weight, significantly higher compared to the 1,000 mg group; had 557 ± 490 cells.mg−1 tissue weight (mean of (n = 3) ± 95% C.l). The isolated cells were analyzed using FACs BD Flowcytometer, expressed cTnT + 13.38%, PECAM-1 + /VCAM-1- 32.25%, cKit + 7.85%, ICAM + 85.53%, indicating the cardiomyocyte progenitor cells. Conclusion: Cardiomyocytes taken from the wasted heart tissue might be a candidate of cardiomyocytes source to study interventions to the heart as it contained up to 13.38% cardiomyocytes, and 32.25% of cardiac progenitor cells. Moreover, perhaps when cardiac cell therapy needs autologous cardiomyocytes, less than 500 mg tissue weight can be considered as sufficient. © 2021, The Author(s), under exclusive licence to Springer Nature B.V. |
Cardiac progenitor cells; Cardiomyocyte isolation; Right ventricular resection; Tetralogy of fallot; Waste heart tissue |
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Springer Science and Business Media B.V. |
13899333 |
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Article |
Q3 |
397 |
12196 |
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681 |
Purbadi S., Tjahjadi H., Purwoto G., Kusuma F., Winarto H., Utami T.W., Indarti J., Sotarduga G.E. |
6505677307;57210953454;14720170400;57192911548;54080645300;57195720528;39161587400;57226332860; |
Early gestational age placenta accreta case report: Can we avoid missed diagnosis? |
2021 |
American Journal of Case Reports |
22 |
1 |
e934168 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118201241&doi=10.12659%2fAJCR.934168&partnerID=40&md5=ad11e65742808074c47abe298cdbe9f7 |
Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Department of Pathology and Anatomy, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Department of Obstetrics and Gynecology, University of Indonesia Dr. Cipto Mangunkusumo National Referral Hospital Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia |
Purbadi, S., Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Tjahjadi, H., Department of Pathology and Anatomy, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Purwoto, G., Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Kusuma, F., Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Winarto, H., Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Utami, T.W., Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Indarti, J., Department of Obstetrics and Gynecology, University of Indonesia Dr. Cipto Mangunkusumo National Referral Hospital Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Sotarduga, G.E., Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia |
Objective: Background: Case Reports: Conclusions: Mistake in diagnosis Placenta accreta is an abnormal invasive placenta that can be life-threatening because of the risk of hemorrhage. Its incidence has increased due to high cesarean delivery rates. Early gestational age placenta accreta is difficult to diagnose and misdiagnosis can lead to inappropriate treatment. Patient 1, a 34-year-old woman (para 2 abortus 1) with 2 previous cesarean deliveries, was referred to our department for vaginal bleeding and abdominal pain. She received 2 curettages for blighted ovum; then, ultrasound examination found uterus perforation and fluid in the Douglas cavity. Exploratory laparotomy confirmed uterine perforation, and a hysterectomy was performed. Histopathological examination revealed placenta accreta. Patient 2, a 35-year-old woman (para 3) with 3 previous cesarean deliveries, was treated at a previous hospital for vaginal bleeding and stomach enlargement. She received serial chemotherapy for gestational trophoblastic neoplasia. Ultrasound examination showed a nonhomogeneous opacity in the lower uterine corpus with color score 4. Total abdominal hysterectomy was performed, and histopathological examination revealed placenta accreta. Patient 3, a 32-year-old woman (para 2) with 2 previous cesarean deliveries, had irregular vaginal bleeding suspected as gestational trophoblastic neoplasia due to ultrasound examination and positive beta-human chorionic gonadotropin. Ultrasound and MRI examination showed enlargement with nonhomogeneous opacity, color score 4, and bridging vessels. Due to our previous experience, we suspected it was a placenta accreta and performed a hysterectomy. The histopathology result indicated placenta accreta. The key point in diagnosing placenta accreta properly is to evaluate the morphometric changes based on the structure using imaging like ultrasound. Collection and analysis of these data enables precise diagnosis in early gestational age placenta accreta. © 2021, International Scientific Information, Inc.. All rights reserved. |
Cesarean section; Diagnostic techniques, obstetrical and gynecological; Placenta accreta |
analgesic agent; antibiotic agent; methotrexate; abdominal hysterectomy; abdominal pain; abdominal swelling; adult; Article; blighted ovum; cancer chemotherapy; case report; cesarean section; choriocarcinoma; clinical article; diagnostic error; dilatation and curettage; Doppler flowmetry; echography; emergency ward; female; fever; follow up; gestational age; histopathology; hospital admission; hospital discharge; human; hypovolemic shock; hysterectomy; laparotomy; missed abortion; nuclear magnetic resonance imaging; patient referral; pelvic inflammatory disease; placenta accreta; rectouterine pouch; spontaneous abortion; spotting; treatment duration; trophoblastic tumor; uterus disease; uterus perforation; vagina bleeding; diagnostic imaging; gestational age; placenta; placenta accreta; pr |
International Scientific Information, Inc. |
19415923 |
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34719664 |
Article |
Q3 |
247 |
16822 |
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682 |
Safri A.Y., Pawitan J.A. |
57091699300;6508348067; |
Nerve tissue engineering in peripheral nerve injury |
2021 |
International Medical Journal |
28 |
1 |
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16 |
19 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118134217&partnerID=40&md5=f829640cb3d11f1c1fcb1f8558832de1 |
Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Departement of Neurology, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/Faculty of Medicine, Universitas Indonesia, Indonesia; Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Indonesia |
Safri, A.Y., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Departement of Neurology, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia; Pawitan, J.A., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/Faculty of Medicine, Universitas Indonesia, Indonesia, Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Indonesia |
Objective: This article aims to explain the mechanism of recovery of peripheral nerve injury, current management and progress in nerve tissue engineering, which are promising to overcome the problems of peripheral nerve injury. Materials and Methods: we searched various sources, i.e. Pubmed, Scopus, and Google scholar to collect relevant publica-tions of peripheral nerve injury and its management, including nerve tissue engineering. Results: The development of tissue engineering has made it possible to regenerate nerve tissue by a combination of cell ther-apy, appropriate design of scaffolds and the use of supporting elements. Discussion: Under current management, severe peripheral nerve injury especially injury to the proximal and large diameter nerves still has a poor prognosis. Poor prognosis is mainly due to a mismatch in nerve autograft in both structure and diameter to the host nerve. The advantage of tissue engineering techniques in the management of peripheral nerve injuries is that the engineered nerve tissue can be designed according to the structure and diameter of the damaged nerve. Conclusion: Nerve tissue engineering might overcome the problems in peripheral nerve regeneration by a combination of stem cell therapy, and appropriate design of scaffolds to provide nerve conduits. © 2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation. |
Peripheral nerve injury; Scaffolds; Stem cell; Tissue engineering |
Article; autograft; cell body; cell differentiation; ganglion; human; nerve cell; nerve fiber regeneration; nerve regeneration; nervous system; peripheral nerve injury; stem cell transplantation; systematic review; tissue engineering; tissue regeneration |
Japan International Cultural Exchange Foundation |
13412051 |
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Article |
Q4 |
183 |
20490 |
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683 |
Puspitasari H.A., Indriasti N., Hidayati E.L. |
57214119502;57310728900;57200542624; |
Antihypertensive management in children: A two-years experience of indonesia's national referral hospital |
2021 |
Anatolian Journal of Family Medicine |
4 |
1 |
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7 |
12 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117862602&doi=10.5505%2fanatoljfm.2020.18209&partnerID=40&md5=bb62f06ed1d11c2f9f176e26ae094521 |
Department of Child Health, Division of Pediatric Nephrology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Puspitasari, H.A., Department of Child Health, Division of Pediatric Nephrology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Indriasti, N., Department of Child Health, Division of Pediatric Nephrology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Hidayati, E.L., Department of Child Health, Division of Pediatric Nephrology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Objectives: The prevalence of hypertension (HT) in children has increased overtime. However, data related to antihypertensive options and outcomes are still limited in children, especially in Indonesia. This study aimed to describe pediatric HT cases and antihypertensive management in Cipto Mangunkusumo General Hospital (CMGH). Methods: A retrospective cross-sectional study was conducted in CMGH from January 2016 to December 2017. Inclusion criteria were children age <18 years with primary and secondary HT visiting pediatric nephrology clinic. Patients were excluded if no blood pressure records were found. Clinical and antihypertensive treatments data were recorded from medical records. Diagnosis was based on the Fourth Report classification. Results: This study included 176 children with HT. Secondary HT was found in 159 (90.3%) patients, and 82 (51.6%) of them had isolated kidney-urinary tract disease. Dual antihypertensive therapy was prescribed for 53 (30.1%) patients, whereas 55 (31.3%) patients was monotherapy. The most common antihypertensive used in monotherapy was angiotensin converting enzyme inhibitors (ACEI), received by 31 (56.3%) patients. The median duration of therapy until achievement of target blood pressure was 28.0 (1.0-365.0) days. Conclusion: Pediatric HT is an often overlooked growing medical issue. Secondary HT was found more in children compare to primary HT. Secondary etiology was suspected if HT was found in children with kidney disease. The first-line antihypertensive drug used was ACEI, as single or combination therapy. The therapeutic effect of antihypertensive drugs was expected within 28 days of treatment. © 2020 by Anatolian Journal of Family Medicine. |
Antihypertensive agents; Children; Essential hypertension; Hypertension |
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Kare Publishing |
26305593 |
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Article |
#N/A |
#N/A |
#N/A |
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684 |
Sukrisman L., Sinto R., Priantono D. |
8661764000;36099377100;57219443427; |
Hematologic profiles and correlation between absolute lymphocyte count and neutrophil/ lymphocyte ratio with markers of inflammation of covid-19 in an indonesian national referral hospital |
2021 |
International Journal of General Medicine |
14 |
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6919 |
6924 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117751905&doi=10.2147%2fIJGM.S337440&partnerID=40&md5=3dd70fba2ee4c7395a09b7807a9e5c42 |
Division of Haematology-Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Division of Tropical and Infectious Disease, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Sukrisman, L., Division of Haematology-Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Sinto, R., Division of Tropical and Infectious Disease, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Priantono, D., Division of Haematology-Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Introduction: COVID-19 is a pandemic with significant mortality and it is important to differentiate severe and non-severe cases. We conducted a study to evaluate hematologic profiles with inflammation markers in COVID-19 patients and to determine the correlation of neutrophil-lymphocyte ratio (NLR) with disease severity. Methods: A cross-sectional study involving hospitalized COVID-19 patients confirmed with a positive SARS-CoV-2 PCR test in Dr. Cipto Mangunkusumo Hospital. Lymphocyte count, NLR, C-reactive protein (CRP) and ferritin were evaluated in severe and non-severe COVID-19 cases at hospital admission. Data was analyzed using Spearman correlation. Results: There were 41 patients aged 20 to 79 years with COVID-19; 33 (80.5%) were nonsevere, and 8 (19.5%) were severe cases. There is a statistically significant difference in WBC, relative neutrophils and lymphocytes, NLR, and CRP between non-severe and severe cases. There is a strong correlation between NLR and CRP (r = 0.738; p < 0.001). Our findings show that NLR and absolute lymphocyte count, but not ferritin, play a role in differentiating between non-severe and severe COVID-19 cases. Conclusion: In COVID-19 cases, a strong correlation between NLR and CRP might suggest the use of NLR to differentiate between non-severe and severe cases, especially in a remote healthcare facility. © 2021 Sukrisman et al. This work is published and licensed by Dove Medical Press Limited. |
COVID-19; Hematologic profiles; Inflammation; Lymphocyte; NLR |
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Dove Medical Press Ltd |
11787074 |
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Article |
Q2 |
722 |
6874 |
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685 |
Lubis A.M.T., Reksoprodjo A.Y., Kuncoro M.W., Ifran N.N. |
15122639800;57208385626;57214136871;56091403800; |
Post-acl reconstruction graft failure in severe gout arthritis patient |
2021 |
International Medical Case Reports Journal |
14 |
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725 |
730 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117588489&doi=10.2147%2fIMCRJ.S325642&partnerID=40&md5=7d6c96304b0f081df9890285db073149 |
Department of Orthopedic & Traumatology, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Lubis, A.M.T., Department of Orthopedic & Traumatology, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Reksoprodjo, A.Y., Department of Orthopedic & Traumatology, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Kuncoro, M.W., Department of Orthopedic & Traumatology, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Ifran, N.N., Department of Orthopedic & Traumatology, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Introduction: Pain and instability following an anterior cruciate ligament (ACL) reconstruction remain a significant issue. Common causes include infection and inflammation, and mechanical issues such as graft failure. Case Presentation: A 36-year-old male with an ACL reconstruction six years prior was admitted due to pain and swelling. The ACL graft that had ruptured was found arthrosco-pically. Numerous gout crystals and tophus were observed inside. This finding is suggested as a cause of this kind of complication. Discussion: Biological failure is one of the causes of graft failure. Gout arthritis is associated with internal derangement of the joint structures, which is reported in advanced case. Conclusion: Severe gouty arthritis, even though rare, could be an etiology of graft failure following ACL reconstruction and cause significant morbidity. © 2021 Lubis et al. |
ACL reconstruction; Gout; Graft failure; Tophus |
urate; adult; anterior cruciate ligament reconstruction; arthroscopic debridement; Article; case report; clinical article; gout; graft failure; hamstring graft; human; human tissue; hyperuricemia; joint effusion; joint limitation; joint stiffness; joint swelling; knee arthroscopy; knee instability; knee pain; knee radiography; male; muscle graft |
Dove Medical Press Ltd |
1179142X |
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Article |
Q4 |
198 |
19482 |
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686 |
Muharam R., Febri R.R., Prabowo K.A., Bustami A., Mansur I.G. |
57191492732;57195941864;57224859187;57192888839;6603222374; |
Increased Levels of CD107a and Intracellular Cytokines in IL-2 Stimulated PBMCs from Endometriosis Patients |
2021 |
International Journal of Inflammation |
2021 |
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5760959 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117385290&doi=10.1155%2f2021%2f5760959&partnerID=40&md5=016a37c79309194717aea380b260bb3e |
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Human Reproduction, Infertility, and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta 10430, Indonesia; Integrated Laboratory of Medical Faculty, Universitas Indonesia, Jakarta, 10430, Indonesia; Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Muharam, R., Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia, Human Reproduction, Infertility, and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia, Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta 10430, Indonesia; Febri, R.R., Human Reproduction, Infertility, and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Prabowo, K.A., Human Reproduction, Infertility, and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Bustami, A., Integrated Laboratory of Medical Faculty, Universitas Indonesia, Jakarta, 10430, Indonesia, Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Mansur, I.G., Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
It has been postulated that the immune system is impaired in individuals with endometriosis, with attention directed to natural killer (NK) cells. Specifically, it has been hypothesized that altered numbers of peripheral NK cells in blood are associated with the presence of endometriotic lesions. This study aimed to evaluate the level of the peripheral NK cell surface marker CD107a in endometriosis in the presence of IL-2 stimulation. Peripheral blood mononuclear cells (PBMCs) were obtained from 7 women with endometriosis and 7 women without endometriosis. The PBMCs were divided into two groups and either treated with recombinant IL-2 or left untreated. The cytotoxic activity of the PBMCs toward target cells (K562) was evaluated. Then, both groups were cocultured for 4 days. The expressions of CD107a, TNF-α, and IFN-γ were determined using flow cytometry analysis. There was no difference in the expression of CD107a prior to IL-2 stimulation in PBMCs from women with endometriosis compared to those from women without endometriosis. However, we observed upregulation of the expression of the surface marker CD107a after treatment in the endometriosis group. In addition, there was a significant difference in CD107a expression in the endometriosis group before versus after stimulation with IL-2 (p < 0.01). We also found no difference in the production of TNF-α and IFN-γ before versus after treatment with IL-2 in either groups. The levels of CD107a were significantly enhanced in peripheral blood taken from women with endometriosis after treatment with IL-2. © 2021 R. Muharam et al. |
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gamma interferon; interleukin 2; lysosome associated membrane protein 1; tumor necrosis factor; adult; Article; cell activation; cell culture; cell isolation; cell stimulation; clinical article; coculture; controlled study; cytokine production; cytotoxicity; endometriosis; female; flow cytometry; human; human cell; immune response; K-562 cell line; pathogenesis; peripheral blood mononuclear cell; protein expression; upregulation |
Hindawi Limited |
20908040 |
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Article |
Q2 |
1106 |
3761 |
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687 |
Chandra R., Pudjiadi A.H., Dewi R. |
57299963800;18435202300;57190859324; |
Citrullinated Histone H3 Level as a Novel Biomarker in Pediatric Clinical Sepsis |
2021 |
Indonesian Biomedical Journal |
13 |
3 |
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316 |
323 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117346746&doi=10.18585%2finabj.v13i3.1597&partnerID=40&md5=18aa6f1cc8a9eaeff375d765e0215f81 |
Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia |
Chandra, R., Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia; Pudjiadi, A.H., Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia; Dewi, R., Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia |
Background: Sepsis is still leading cause of death in critically ill children. Early recognition of sepsis and treatments are needed to reduce its mortality. The use of citrullinated Histone H3 (Cit-H3) as an early sepsis marker and outcome predictor has been validated in previous studies among adults. However, only one study in pediatric meningococcal sepsis was reported with contradictory results. This study aims to determine Cit-H3 levels in pediatric clinical sepsis and analyze its association with sepsis severity and survival rate. METHODS: A prospective observational cohort study involving 67 pediatric subjects clinically diagnosed sepsis was conducted. Cit-H3 levels, Pediatric Logistic Organ Dysfunction-2 (PELOD-2) score, and Pediatric Sequential Organ Failure Assessment (pSOFA) score were assessed at the time of diagnosis (0-hour) and 48 hours later. Pearson Correlation test was used to determine the correlation between Cit-H3 levels with PELOD-2 andpSOFA scores and receiver operating curve to find the cutoff of Cit-H3 levels on clinical sepsis patients. RESULTS: Among clinically sepsis patients, the median Cit-H3 level was 1,210 (800-32,160) ng/mL, with optimal cut-off point ≥1200 ng/mL (sensitivity 83.3% and specificity 75.7%) to discriminate sepsis. The median Cit-H3 levels at 0-hour were lower in survivor compared to non-survivor group (p=0.016). Cit-H3 level was able to predict mortality with optimal cut-off point ≥1,200 ng/mL, sensitivity 72.2% and specificity 57.1% (AUC of 69.2%; p=0.017). Using survival analysis, Cit-H3 was significantly associated with the mortality rate (p=0.023; hazard ratio of 3.45). CONCLUSION: Cit-H3 level could be potential to predict pediatric sepsis events and its outcome. © 2021 |
citrullinated histone H3; neutrophil extracellular traps; pediatric sepsis; PELOD-2 score; pSOFA score; survival |
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Prodia Education and Research Institute |
20853297 |
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Article |
Q4 |
160 |
22228 |
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688 |
Bachtiar B.M., Theodorea C.F., Tahapary D.L., Astrella C., Natalina, Bachtiar E.W. |
15831189400;57190048861;55944492500;57210793320;57193344580;6507328763; |
A pilot study of red complex and three genera subgingival microbiome in periodontitis subjects with and without diabetes, evaluated by MinION platform |
2021 |
F1000Research |
10 |
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1 |
23 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117343626&doi=10.12688%2fF1000RESEARCH.28216.4&partnerID=40&md5=67091555cb3a43a5a6d2e958586434d2 |
Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia; Division of Endocrinology, Department of Internal Medicine, Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Metabolic, Cardiovascular and Aging Cluster, The Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Internal Medicine, Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia |
Bachtiar, B.M., Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia; Theodorea, C.F., Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia; Tahapary, D.L., Division of Endocrinology, Department of Internal Medicine, Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia, Metabolic, Cardiovascular and Aging Cluster, The Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Astrella, C., Metabolic, Cardiovascular and Aging Cluster, The Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Internal Medicine, Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Natalina, Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia; Bachtiar, E.W., Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia |
Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes. Methods: The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data. Results: We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups. Conclusions: Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes. © 2021. Bachtiar BM et al. |
16S rRNA; Diabetes; MinION; Periodontitis; Red Complex bacteria; Subgingival Microbiome |
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F1000 Research Ltd |
20461402 |
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Article |
Q1 |
1099 |
3793 |
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689 |
Kartika R.W., Alwi I., Suyatna F.D., Sandra F., Yunir E., Waspadji S., Immanuel S., Silalahi T., Sungkar S., Rachmat J., Bardosono S., Reksodiputro M.H. |
57223447932;15055173800;56039633100;56016164200;36520254800;8678136400;57272979000;56275255400;57016857300;6507225348;21933841000;35090488800; |
Hyaluronic Acid Accelerates VEGF and PDGF Release from Advance Platelet Rich Fibrin in Diabetic Foot Ulcer |
2021 |
Indonesian Biomedical Journal |
13 |
3 |
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332 |
336 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117336402&doi=10.18585%2finabj.v13i3.1523&partnerID=40&md5=8bff3d535a0c8b7bbd27fc12d2903eb5 |
Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Department of Internal Medicine, Faculty of Medicine, Krida Wacana Christian University, Jl. Tanjung Duren Raya No.4, Jakarta, Indonesia; Department of Clinical Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Facial Plastic Reconstructive Division, Department of Otorhinolaryngology, Faculty of Medicine, Universitas Indonesia –Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia |
Kartika, R.W., Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia, Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Alwi, I., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Suyatna, F.D., Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Sandra, F., Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta, Indonesia; Yunir, E., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Waspadji, S., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Immanuel, S., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Silalahi, T., Department of Internal Medicine, Faculty of Medicine, Krida Wacana Christian University, Jl. Tanjung Duren Raya No.4, Jakarta, Indonesia; Sungkar, S., Department of Clinical Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Rachmat, J., Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Bardosono, S., Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Reksodiputro, M.H., Facial Plastic Reconstructive Division, Department of Otorhinolaryngology, Faculty of Medicine, Universitas Indonesia –Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia |
Background: Hyaluronic acid (HA) is an essential component of extracellular matrix and mediates signaling in wound healing. HA could induce growth factor release from Advanced Platelet Rich Fibrin (A-PRF), including Vascular Endothelial Growth Factor (VEGF) and Platelet-derived Growth Factor (PDGF). However, concentrations of the released-VEGF and PDGF have not been clearly disclosed. Therefore, current study was conducted to measure the release of these growth factors in HA + A-PRF gel of diabetic foot ulcer (DFU) subjects. Methods: Twenty DFU subjects were included in the study and treated with A-PRF or HA+A-PRF. A-PRF was derived from autologous peripheral blood and processed with low-speed centrifugation. HA was added with a ratio of 1:0.6. A-PRF or HA + A-PRF was applied topically on DFU. Upper tips of A-PRF or HA + A-PRF gels were collected on day 0, 3 and 7 for measurements of VEGF and PDGF concentrations with Enzyme-linked Immune-sorbent Assay (ELISA) methods. Results: On day-3, both VEGF and PDGF concentrations of HA + A-PRF group were significantly higher than the VEGF (p=0.000) and PDGF (p=0.019) concentrations of A-PRF group. The VEGF and PDGF concentrations were continuously and significantly increased on day-7 of HA + A-PRF group, compared to the VEGF (p=0.000) and PDGF (p=0.004) concentrations of A-PRF group. Conclusion: Combination HA+A-PRF induces VEGF and PDGF release from A-PRF. A mixture of A-PRF and HA could be more effective than A-PRF alone for treatment of DFU. © 2021. The Indonesian Biomedical Journal. All Rights Reserved. |
advanced platelet rich fibrin; diabetic foot ulcer; growth factor; hyaluronic acid; PDGF; PRF; VEGF |
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Prodia Education and Research Institute |
20853297 |
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Article |
Q4 |
160 |
22228 |
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