No records
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35 |
Dwijayanti A., Fitrianto A.I., Boediono A., Setiati S., Suyatna F.D. |
55743787900;57377401300;9040094200;14325991900;56039633100; |
Mesenchymal stem cells administration in aged male rats increases testosterone and lower TNF-α levels |
2021 |
International Medical Journal |
28 |
6 |
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601 |
603 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121461058&partnerID=40&md5=bbf2100ed3003ab64dcb298ba7d9f029 |
Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia; Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Dwijayanti, A., Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Fitrianto, A.I., Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia; Boediono, A., Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia; Setiati, S., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Suyatna, F.D., Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Background: Treatment for sex hormone depletion along with chronic inflammation would be beneficial for aging males. Mesenchymal stem cells (MSC) are known for their immunomodulatory activities and differentiation ability in regenerative medicine. Whether MSC could influence both testosterone and pro-inflammatory marker in aging males is uncertain. This study aimed to explore the effects of human umbilical mesenchymal stem cell (hUCMSC) on testosterone, tumor necrosis factor-alpha (TNF-α), and creatinine levels in aged rats. Methods: The hUCMSC was administered to aged male Sprague-Dawley rats (24 months old). After four injections of 1 mil-lion per kg body weight in 3-month intervals, the rats were sacrificed, and serum was collected for biochemical examinations. Results: The hUCMSC administrations increased the testosterone level almost three-fold and decreased the TNF-α level. Moreover, the high testosterone level was strongly correlated with low TNF-α level (p = 0.013; r =-0.863) in aged male rats. These results were following our previous report, which showed that the hUCMSC increased the number of Leydig cells. Serum creatinine levels in the treatment group were slightly increased but were still within the normal limit. Conclusion: The hUCMSC treatment in aged male rats tends to increase testosterone levels and lower TNF-α levels. © 2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation. |
Aging; Mesenchymal stem cell; Testosterone; TNF-α |
creatinine; sex hormone; testosterone; tumor necrosis factor; aged; aging; animal cell; animal experiment; animal model; animal tissue; Article; body weight; controlled study; creatinine blood level; creatinine clearance; female; gene expression; Leydig cell; male; mesenchymal stem cell; nonhuman; protein expression; rat; regenerative medicine; Sprague Dawley rat; testosterone blood level |
Japan International Cultural Exchange Foundation |
13412051 |
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Article |
Q4 |
183 |
20490 |
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145 |
Hansur L., Pawitan J.A. |
57296411600;6508348067; |
The potential of hydroxysafflor yellow a as an adjuvant in covid-19 patients with acute respiratory distress syndrome |
2021 |
International Medical Journal |
28 |
5 |
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527 |
531 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117118067&partnerID=40&md5=caa56a92d25ef57cc5e5c3533887adf1 |
Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Muhammadiyah Makassar, Makassar, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/ Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Hansur, L., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Medicine, Universitas Muhammadiyah Makassar, Makassar, Indonesia; Pawitan, J.A., Department of Histology, Faculty of Medicine, Universitas Indonesia, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/ Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Objective: To discuss the potential and molecular mechanism of Carthamus tincorius derived hydroxysafflor yellow A (HSYA) as an alternative herbal adjuvant that may regulate various signaling pathways that might be related to the regulatory effects in COVID-19 patients with ALI and ARDS. Methods: We search Web of Science, PubMed, and Scopus using keywords: Carthamus tinctorius, adjuvant, cytokine storm, COVID-19, SARS-Cov-2, acute lung injury (ALI), and Acute Respiratory Distress Syndrome (ARDS), on 11 September 2020, and 18 December 2020. Results and Discussions: In COVID-19 patients, SARS-CoV-2 replication might be associated with hyper induction of pro-in-flammatory cytokine, which is known as a cytokine storm, and may cause acute lung injury (ALI) that leads to Acute Respiratory Distress Syndrome (ARDS). Carthamus tincorius derived HSYA were used in many studies, in vivo in animal models or in vitro in cell lines and showed inhibition of multiple inflammatory pathways that were involved in ALI and ARDS, which might occur in covid-19 patients. HSYA showed pleiotropic effects in regulating cytokine levels. It regulated TNF-α, IL-1β, IL-6, IFN-β, and showed protective effect by blocking TLR4, MyD88, TRIF, IRF3, NF-κB to avoid cytokine storm and prevent tissue damage. HSYA was showed to reduce oxidative stress-mediated damage, and down-regulate inflammatory cytokines. Further, it was relatively safe when studied as an adjuvant in HIV and cancer patients. Conclusion: We supposed that HSYA could be used as an alternative adjuvant in COVID-19 patients with ARDS. However, clinical trials are needed to prove its efficacy in COVID-19 patients with ARDS. © 2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation. |
Carthamus tinctorius; COVID-19; Cytokine storm; HSYA; SARS-Cov-2 |
adjuvant; curcumin; hydroxysafflor yellow a; interleukin 1beta; interleukin 6; interleukin 8; myeloid differentiation factor 88; natural product; pathogen associated molecular pattern; toll like receptor 4; tumor necrosis factor; unclassified drug; acute lung injury; adult respiratory distress syndrome; antiinflammatory activity; apoptosis; Article; binding affinity; brain ischemia; cancer patient; coronavirus disease 2019; cytokine production; cytokine storm; down regulation; dyspnea; endothelial dysfunction; endothelium cell; enzyme activity; human; Human immunodeficiency virus; immune response; inflammation; lung edema; nonhuman; pleiotropy; protein expression; protein function; reperfusion injury; respiratory failure; Severe acute respiratory syndrome coronavirus 2; signal transduction |
Japan International Cultural Exchange Foundation |
13412051 |
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Article |
Q4 |
183 |
20490 |
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146 |
Dwijayanti A., Pawitan J.A. |
55743787900;6508348067; |
Mesenchymal stem cell aging, their environment and methods to restore their quality |
2021 |
International Medical Journal |
28 |
5 |
|
520 |
523 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117117062&partnerID=40&md5=e4cc00f4efb942f9321160eff1b790a8 |
Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Dwijayanti, A., Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Pawitan, J.A., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Objective: This article aims to explore the aging of mesenchymal stem cells (MSCs) during cell expansion and available methods to restore their quality. Materials and methods: we searched articles in PubMed/Medline and our library to collect relevant publications of MSC aging, their microenvironment (niche), and various approaches to prevent MSC aging. Results and Discussion: MSC expansion during culture may cause cell aging, which is reflected by change in growth, geno-type, and phenotype that can be seen in morphology and function. There are various approaches that can be used to prevent MSC aging. Approaches to prevent MSC aging in culture can be done by various methods, including by determining donor characteristics, genetic engineering approaches, regulating stem cells microenvironment in vitro, and in vitro physical modification Conclusion: Prevention of MSC aging during culture can be done by selection of MSC sources, genetic modification, and microenvironment and physical adjustments. ©2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation. |
Aging; Cell culture; Mesenchymal stem cells; Micro environment; Propagation |
adult; article; cell aging; cell culture; DNA modification; genetic engineering; human; human cell; Medline; mesenchymal stem cell; microenvironment; phenotype; stem cell expansion |
Japan International Cultural Exchange Foundation |
13412051 |
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Article |
Q4 |
183 |
20490 |
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355 |
Purwoningsih E., Pawitan J.A. |
57224985986;6508348067; |
Mesenchymal stem cells: Potential application in covid-19 patients with acute respiratory distress syndrome (ards) |
2021 |
International Medical Journal |
28 |
3 |
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303 |
306 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108812441&partnerID=40&md5=0feffaf110f53271a8fb3d884ed44c7c |
Biomedical Science, Faculty of Medicine, Universitas Indonesia, Indonesia; Faculty of Medicine, Universitas Muhammadiyah Sumatera Utara, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Indonesia; Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine Universitas Indonesia, Indonesia |
Purwoningsih, E., Biomedical Science, Faculty of Medicine, Universitas Indonesia, Indonesia, Faculty of Medicine, Universitas Muhammadiyah Sumatera Utara, Indonesia; Pawitan, J.A., Department of Histology, Faculty of Medicine, Universitas Indonesia, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Indonesia, Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine Universitas Indonesia, Indonesia |
Objective: To highlight the potential of mesenchymal stem cells (MSCs) as an adjuvant therapy for severe COVID-19 patient. Materials and Methods: We searched Pubmed and Google Scholar, using keywords: 'MSC AND lung injury', 'MSC AND ARDS', 'MSC AND COVID-19', 'Stem cell AND Lung injury' and 'Stem cell AND ARDS', on 19 Mei and 19 July 2020. Results and Discussions: Severe manifestations in COVID-19 patients are due to immune system response. MSCs have immunomodulatory and anti-inflammatory effect and therefore may be beneficial to alleviate acute respiratory distress syndrome (ARDS). A small published study showed that MSCs had beneficial effect on COVID-19 patients, who showed clinical symptom improvements. Further, application of MSCs from several sources such as bone marrow, menstrual blood, and umbilical cord-derived MSCs, which were used in patients suffering from lung injury/ARDS due to conditions other than COVID-19, showed that a dose of up to 1.0 x 107 cells/kg body weight was well tolerated. Conclusion: administration of MSCs to COVID-19 patients showed improvement in clinical symptoms, and a dose up to 1.0 x 107 cells/kg body weight showed tolerance in ARDS patients with moderate to severe conditions. However, the results came from studies with small number of patients, so the results need to be interpreted with caution, and more well design studies with a larger number of patients are needed. © 2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation. |
Coronavirus; COVID-19; Mesenchymal stem cell; Stem cells |
alpha interferon; angiopoietin; angiotensin converting enzyme 2; beta interferon; bradykinin; C reactive protein; cytokine; gamma interferon inducible protein 10; granulocyte colony stimulating factor; interferon; interleukin 1; interleukin 10; interleukin 13; interleukin 1beta; interleukin 2 receptor; interleukin 4; interleukin 6; keratinocyte growth factor; monocyte chemotactic protein 1; receptor binding domain; toll like receptor; transmembrane protease serine 2; tumor necrosis factor; unclassified drug; vasculotropin; viral protein; virus spike protein; adjuvant therapy; adult respiratory distress syndrome; angiogenesis; Article; B lymphocyte; CD8+ T lymphocyte; cell differentiation; coronavirus disease 2019; cytokine storm; dendritic cell; exosome; genetic transcription; human; immun |
Japan International Cultural Exchange Foundation |
13412051 |
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Article |
Q4 |
183 |
20490 |
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682 |
Safri A.Y., Pawitan J.A. |
57091699300;6508348067; |
Nerve tissue engineering in peripheral nerve injury |
2021 |
International Medical Journal |
28 |
1 |
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16 |
19 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118134217&partnerID=40&md5=f829640cb3d11f1c1fcb1f8558832de1 |
Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Departement of Neurology, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/Faculty of Medicine, Universitas Indonesia, Indonesia; Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Indonesia |
Safri, A.Y., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Departement of Neurology, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia; Pawitan, J.A., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/Faculty of Medicine, Universitas Indonesia, Indonesia, Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Indonesia |
Objective: This article aims to explain the mechanism of recovery of peripheral nerve injury, current management and progress in nerve tissue engineering, which are promising to overcome the problems of peripheral nerve injury. Materials and Methods: we searched various sources, i.e. Pubmed, Scopus, and Google scholar to collect relevant publica-tions of peripheral nerve injury and its management, including nerve tissue engineering. Results: The development of tissue engineering has made it possible to regenerate nerve tissue by a combination of cell ther-apy, appropriate design of scaffolds and the use of supporting elements. Discussion: Under current management, severe peripheral nerve injury especially injury to the proximal and large diameter nerves still has a poor prognosis. Poor prognosis is mainly due to a mismatch in nerve autograft in both structure and diameter to the host nerve. The advantage of tissue engineering techniques in the management of peripheral nerve injuries is that the engineered nerve tissue can be designed according to the structure and diameter of the damaged nerve. Conclusion: Nerve tissue engineering might overcome the problems in peripheral nerve regeneration by a combination of stem cell therapy, and appropriate design of scaffolds to provide nerve conduits. © 2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation. |
Peripheral nerve injury; Scaffolds; Stem cell; Tissue engineering |
Article; autograft; cell body; cell differentiation; ganglion; human; nerve cell; nerve fiber regeneration; nerve regeneration; nervous system; peripheral nerve injury; stem cell transplantation; systematic review; tissue engineering; tissue regeneration |
Japan International Cultural Exchange Foundation |
13412051 |
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Article |
Q4 |
183 |
20490 |
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No records
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898 |
Dewi B.E., Nainggolan L., Sudiro T.M., Chenderawasi S., Goentoro P.L., Sjatha F. |
24076058600;23498394800;6506414225;57221643087;57212515515;55372815000; |
Circulation of various dengue serotypes in a community-based study in Jakarta, Indonesia |
2021 |
Japanese Journal of Infectious Diseases |
74 |
1 |
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17 |
22 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099705269&doi=10.7883%2fyoken.JJID.2019.431&partnerID=40&md5=d7c98adae43cb2a3728e97343d353000 |
Department of Microbiology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Departement of Internal Medicine, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Community Based Dengue Study, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Cluster of Infectious Diseases and Immunology, Indonesian Medical Education and Research Institute (IMERI), Jakarta, Indonesia |
Dewi, B.E., Department of Microbiology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Community Based Dengue Study, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Cluster of Infectious Diseases and Immunology, Indonesian Medical Education and Research Institute (IMERI), Jakarta, Indonesia; Nainggolan, L., Departement of Internal Medicine, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Community Based Dengue Study, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Sudiro, T.M., Department of Microbiology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Cluster of Infectious Diseases and Immunology, Indonesian Medical Education and Research Institute (IMERI), Jakarta, Indonesia; Chenderawasi, S., Community Based Dengue Study, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Goentoro, P.L., Community Based Dengue Study, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Sjatha, F., Department of Microbiology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Cluster of Infectious Diseases and Immunology, Indonesian Medical Education and Research Institute (IMERI), Jakarta, Indonesia |
Dengue virus (DENV) infection remains to be a serious health problem in Indonesia. Community-based dengue studies to determine circulating DENV serotypes based on the geography and season are limited owing to the expensive cost and significant effort required. Many patients with DENV infection are not hospitalized and many visit the hospital in the later phase of the disease. In this study, we performed active DENV surveillance in a community in Jakarta to study the circulating dengue serotypes; adult febrile patients with fever less than 48 hours were recruited. Disease severity was defined using the World Health Organization (WHO) 1997 guidelines. Rapid NS1 dengue antigen detection was used to screen patients with DENV in the community. Viral culture using the C6/36 cell line, an increased antibody titer on hemagglutination inhibition test and enzyme linked immunosorbent assay, or detection of the viral genome on reverse transcription-polymerase chain reaction was used to confirm DENV infection. Of the 102 patients, 68 (66.7%) were confirmed to have DENV infection, with DENV-2 being the most dominant serotype, followed by DENV-3, DENV-1, and DENV-4, in concordance with several reports of mixed DENV infection. Interestingly, in terms of disease severity, although DENV-3 infection was not the predominant circulating serotype, infection with it tended to cause a more severe disease than infection with DENV-2. © 2021, National Institute of Health. All rights reserved. |
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DNA polymerase; nonstructural protein 1; viral protein; virus antibody; adult; antibody titer; antigen detection; Article; Dengue virus; disease severity; enzyme linked immunosorbent assay; female; fever; hemagglutination inhibition test; human; human cell; Indonesia; major clinical study; male; monitoring; reverse transcription polymerase chain reaction; serotype; virus culture; virus genome; World Health Organization; adolescent; dengue; Dengue virus; genetics; isolation and purification; middle aged; molecular epidemiology; severity of illness index; virology; young adult; Adolescent; Adult; Antibodies, Viral; Dengue; Dengue Virus; Enzyme-Linked Immunosorbent Assay; Female; Genome, Viral; Humans; Indonesia; Male; Middle Aged; Molecular Epidemiology; Reverse Transcriptase Polymerase Chai |
National Institute of Health |
13446304 |
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32611971 |
Article |
Q3 |
517 |
9793 |
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No records
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390 |
Umbarawan Y., Enoura A., Ogura H., Sato T., Horikawa M., Ishii T., Sunaga H., Matsui H., Yokoyama T., Kawakami R., Maeno T., Setou M., Kurabayashi M., Iso T. |
57196077830;57224226756;57224226814;57202946030;57195494134;57224227073;55061468300;57212330485;7403358134;57210447153;35407637300;14326068500;7103371684;7003498756; |
Fabp5 is a sensitive marker for lipid-rich macrophages in the luminal side of atherosclerotic lesions |
2021 |
International Heart Journal |
62 |
3 |
|
666 |
676 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107318921&doi=10.1536%2fihj.20-676&partnerID=40&md5=50711618426db51f56d9cb4d728fc9ca |
Department of Cardiovascular Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan; Department of Internal Medicine, Faculty of Medicine Universitas IndonesiaJakarta, Indonesia; Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan; Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, Hamamatsu, Japan; Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan; Center for Liberal Arts and Sciences, Ashikaga University, Ashikaga, Japan; Department of Allergy and Respiratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan; Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Hamamatsu, Japan |
Umbarawan, Y., Department of Cardiovascular Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan, Department of Internal Medicine, Faculty of Medicine Universitas IndonesiaJakarta, Indonesia; Enoura, A., Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan; Ogura, H., Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan; Sato, T., Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan, International Mass Imaging Center, Hamamatsu University School of Medicine, Hamamatsu, Japan; Horikawa, M., Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan, International Mass Imaging Center, Hamamatsu University School of Medicine, Hamamatsu, Japan, Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan; Ishii, T., Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan; Sunaga, H., Department of Cardiovascular Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan, Center for Liberal Arts and Sciences, Ashikaga University, Ashikaga, Japan; Matsui, H., Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan; Yokoyama, T., Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan; Kawakami, R., Department of Cardiovascular Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan; Maeno, T., Department of Allergy and Respiratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan; Setou, M., Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan, International Mass Imaging Center, Hamamatsu University School of Medicine, Hamamatsu, Japan, Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Hamamatsu, Japan; Kurabayashi, M., Department of Cardiovascular Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan; Iso, T., Department of Cardiovascular Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan |
Lipid-rich macrophages in atherosclerotic lesions are thought to be derived from myeloid and vascular smooth muscle cells. A series of studies with genetic and pharmacological inhibition of fatty acid binding protein 4 (FABP4) and FABP5 and bone marrow transplant experiments with FABP4/5 deficient cells in mice have demonstrated that these play an important role in the development of atherosclerosis. However, it is still uncertain about the differential cell-type specificity and distribution between FABP4- and FABP5-expressing cells in early- and late-stage atherosclerotic lesions. In this study, we first explored spatial distribution of FABP4/5 in atherosclerotic lesions in apolipoprotein E deficient (ApoE-/-) mice. FABP4 was only marginally detected in early and advanced lesions, whereas FABP5 was abundantly expressed in these lesions. In advanced lesions, the FABP5-positive area was mostly restricted to the foam cell layer adjacent to the lumen above collagen and elastic fibers with a high signal/noise ratio. Oil red O (ORO) staining revealed that FABP5-positive cells were lipidrich in early and advanced lesions. Together, most of lipid-rich FABP5-positive cells reside adjacent to the lumen above collagen and elastic fibers. We next studied involvement of FABP5 in lesion formation of atherosclerosis using ApoE-/- FABP5-/- mice. However, deletion of FABP5 did not affect the development of atherosclerosis. These findings, along with previous reports, suggest a novel notion that FABP5 is a sensitive marker for bone marrow-derived lipid-rich macrophages in the luminal side of atherosclerotic lesions, although its functional significance remains elusive. © 2021, International Heart Journal Association. All rights reserved. |
Apolipoprotein E knockout mice; Atherosclerosis; Foam cell; Oil red O staining |
apolipoprotein E; CD68 antigen; collagen; fatty acid binding protein 4; fatty acid binding protein 5; Ki 67 antigen; Mac 3; smooth muscle actin; unclassified drug; Fabp4 protein, mouse; Fabp5 protein, mouse; fatty acid binding protein; tumor protein; adipogenesis; animal cell; animal experiment; animal model; animal tissue; apolipoprotein E knockout mouse; Article; atherosclerotic plaque; blood vessel wall; bone marrow transplantation; electrospray mass spectrometry; foam cell; image analysis; immunohistochemistry; lipid rich macrophage; lipid storage; macrophage; mouse; nonhuman; oil red O staining; signal noise ratio; staining; tissue preparation; vascular smooth muscle cell; animal; atherosclerosis; immunology; metabolism; Animals; Atherosclerosis; Fatty Acid-Binding Proteins; Foam Cell |
International Heart Journal Association |
13492365 |
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33994513 |
Article |
Q2 |
555 |
9100 |
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