No records
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670 |
Mustika R., Yo E.C., Faruqi M., Zhuhra R.T. |
57220273147;57213188908;57339401900;57220591134; |
Evaluating the relationship between online learning environment and medical students’ wellbeing during covid-19 pandemic |
2021 |
Malaysian Journal of Medical Sciences |
28 |
5 |
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108 |
117 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119154731&doi=10.21315%2fmjms2021.28.5.11&partnerID=40&md5=d5595e080d252a1b132526ffc8da06b5 |
Medical Education Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia; Medical Education Collaboration Cluster, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia; Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Mustika, R., Medical Education Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia, Medical Education Collaboration Cluster, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia, Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Yo, E.C., Medical Education Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia; Faruqi, M., Medical Education Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia; Zhuhra, R.T., Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Background: The coronavirus disease 2019 (COVID-19) pandemic has been found to negatively affect medical students’ wellbeing. This finding may be related to how medical education is being conducted at present, with online learning replacing face-to-face teaching in many countries. This cross-sectional study aims to assess how the online learning environment is connected to medical students’ wellbeing. Methods: A self-administered online questionnaire was distributed to undergraduate medical students at Universitas Indonesia. The study was conducted from September 2020 to February 2021. The questionnaire included a modified version of the Online Learning Environment Scale (OLES) and the Positive Emotion, Engagement, Relationships, Meaning and Accomplishment (PERMA) profiler. The OLES was used to evaluate students’ perceptions of the online learning environment, whereas the PERMA Profiler was used to evaluate students’ wellbeing. We validated the questionnaire before distribution. The content validity index was 1.0, with internal consistency coefficients of 0.87 and 0.89, respectively. Regression analyses were performed to evaluate the relationship between OLES and PERMA scores. Results: The questionnaire was completed by 274 undergraduate medical students. Students reported moderate to high degrees of positive perception towards online learning, high levels of positive emotions and moderate levels of negative emotions. Statistically significant differences were found across groups based on students’ gender, year of study and academic programme. Almost all aspects of the online learning environment were significantly predictive of students’ wellbeing, with personal relevance and evaluation and assessment being the two most important predictors (R2 = 0.201; P < 0.001). Conclusion: Medical students generally enjoyed online learning, although some challenges were presented. The online learning environment was positively associated with students’ wellbeing; however, some students expressed negative emotions including loneliness, anxiety, anger and sadness. © 2021, Penerbit Universiti Sains Malaysia. All rights reserved. |
COVID-19; Medical students; Online learning environment; Wellbeing |
adult; anger; anxiety; Article; behavioral synchrony; coronavirus disease 2019; cross-sectional study; demography; e-learning; emotion; female; health equity; human; human experiment; learning; learning environment; loneliness; male; medical education; medical student; normal human; pandemic; patient engagement; psychological well-being; questionnaire; sadness; social interaction; undergraduate student; validation study; wellbeing |
Penerbit Universiti Sains Malaysia |
1394195X |
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Article |
Q3 |
394 |
12287 |
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717 |
Kadharusman M.M., Antarianto R.D., Hardiany N.S. |
57222724597;57190862806;57192910605; |
A review of the impact of calorie restriction on stem cell potency |
2021 |
Malaysian Journal of Medical Sciences |
28 |
4 |
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5 |
13 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114744628&doi=10.21315%2fmjms2021.28.4.2&partnerID=40&md5=f082ca9d61a167cf3fec646a0e6012b7 |
Undergraduate Program in Medical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Kadharusman, M.M., Undergraduate Program in Medical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Antarianto, R.D., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Hardiany, N.S., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Calorie restriction (CR) prolongs lifespan in various species and also minimises pathologies caused by aging. One of the characteristics seen in age-related pathologies is stem cell exhaustion. Here, we review the various impacts of CR on mammalian health mediated through stem cell potency in various tissues. This study comprised of a literature search through NCBI, Science Direct, Google Scholar and PubMed, focusing on the impact of CR on pluripotency. In the skeletal muscle, CR acts as an anti-inflammatory agent and increases the presence of satellite cells endogenously to improve regeneration, thus causing a metabolic shift to oxidation to meet oxygen demand. In the intestinal epithelium, CR suppresses the mechanistic target of rapamycin complex 1 (mTORC1) signalling in Paneth cells to shift the stem cell equilibrium towards self-renewal at the cost of differentiation. In haematopoiesis, CR prevents deterioration or maintains the function of haematopoietic stem cells (HSCs) depending on the genetic variation of the mice. In skin and hair follicles, CR increases the thickness of the epidermis and hair growth and improves hair retention through stem cells. CR mediates the proliferation and self-renewal of stem cells in various tissues, thus increasing its regenerative ability. © Penerbit Universiti Sains Malaysia, 2021. |
Calorie restriction; Pluripotency; Stem cell |
apoptosis; caloric restriction; cell differentiation; cell proliferation; deterioration; epidermis; fluorescence activated cell sorting; gene expression; genetic variation; hair follicle; hematopoietic stem cell; insulin sensitivity; intestine epithelium; microenvironment; nonhuman; oxidative phosphorylation; oxidative stress; oxygen consumption; physical activity; regenerative ability; Review; signal transduction; skeletal muscle; stem cell; thermal conductivity; upregulation |
Penerbit Universiti Sains Malaysia |
1394195X |
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Review |
Q3 |
394 |
12287 |
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807 |
Jusman S.W.A., Azzizah I.N., Sadikin M., Hardiany N.S. |
36518792100;57223677370;57205093115;57192910605; |
Is the mitochondrial function of keloid fibroblasts affected by cytoglobin? |
2021 |
Malaysian Journal of Medical Sciences |
28 |
2 |
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39 |
47 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106010069&doi=10.21315%2fmjms2021.28.2.4&partnerID=40&md5=8c4df0a7d2cdcd87611b189bf2440f21 |
Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine Universitas Indonesia, Indonesia; Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Jusman, S.W.A., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Azzizah, I.N., Faculty of Medicine Universitas Indonesia, Indonesia; Sadikin, M., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Hardiany, N.S., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Background: A keloid is a benign skin tumour characterised by excessive proliferation of fibroblasts, a process that requires a sufficient amount of energy. The energy needs are associated with adequate oxygen (O2) flow and well-functioning mitochondria. It is known that cytoglobin (CYGB) has a function in O2 distribution. The aim of the present study was to explore whether the inhibition of CYGB expression caused impaired mitochondrial function of keloid fibroblasts. Methods: An in vitro study was conducted on a keloid fibroblast derived from our previous study. The study was carried out in the laboratory of the Biochemistry & Molecular Biology Department, Faculty of Medicine, Universitas Indonesia (FMUI), from July to December 2018. CYGB expression was inhibited by small interfering ribonucleic acid (siRNA) and CYGB. Analysis of mitochondrial function was observed through peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), a mitochondrial biogenesis marker and the activity of the succinate dehydrogenase (SDH) enzyme in mitochondria. Results: The CYGB gene and protein were downregulated after treatment with CYGB siRNA. Inhibition of CYGB expression with siRNA also tended to decrease the levels of PGC-1α messenger ribonucleic acid (mRNA) and protein, as well as SDH enzyme activity. Conclusion: Inhibition of CYGB expression with siRNA tended to decrease mitochondrial biogenesis and function. This may be useful for understanding the excessive proliferation of fibroblasts in keloids and for development of treatment for keloids. © Penerbit Universiti Sains Malaysia, 2021. |
CYGB siRNA; Keloid fibroblasts; PGC-1α; SDH enzyme activity |
cytoglobin; oxygen; peroxisome proliferator activated receptor gamma coactivator 1alpha; small interfering RNA; succinate dehydrogenase; Article; cell function; cell proliferation; colorimetry; controlled study; down regulation; enzyme activity; enzyme linked immunosorbent assay; fibroblast; gene expression; genetic transfection; human; human cell; in vitro study; keloid; mitochondrial biogenesis; mitochondrial genetics; mRNA expression level; protein expression; protein isolation; real time polymerase chain reaction; reverse transcription; RNA isolation |
Penerbit Universiti Sains Malaysia |
1394195X |
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Article |
Q3 |
394 |
12287 |
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No records
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633 |
Nadia Y.F., Syahrani R.A., Arumsari S., Sadikin M., Wanandi S.I. |
57428347800;57204103147;57193202336;36981411100;36099320700; |
Effect of Cell Culture Medium on the Proliferation and Stemness of CD24-/CD44+ Human Breast Cancer Stem Cells |
2021 |
Indonesian Biomedical Journal |
13 |
4 |
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355 |
363 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123550895&doi=10.18585%2fINABJ.V13I4.1674&partnerID=40&md5=f0c363bff3a069d364bc71970a109469 |
Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia |
Nadia, Y.F., Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Syahrani, R.A., Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Arumsari, S., Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Sadikin, M., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Wanandi, S.I., Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia |
BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24-/CD44+ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES. © 2021. All Rights Reserved. |
breast cancer; cancer stem cell; OCT4; proliferation; stemness |
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Prodia Education and Research Institute |
20853297 |
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Article |
Q4 |
160 |
22228 |
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687 |
Chandra R., Pudjiadi A.H., Dewi R. |
57299963800;18435202300;57190859324; |
Citrullinated Histone H3 Level as a Novel Biomarker in Pediatric Clinical Sepsis |
2021 |
Indonesian Biomedical Journal |
13 |
3 |
|
316 |
323 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117346746&doi=10.18585%2finabj.v13i3.1597&partnerID=40&md5=18aa6f1cc8a9eaeff375d765e0215f81 |
Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia |
Chandra, R., Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia; Pudjiadi, A.H., Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia; Dewi, R., Department of Child Health, Cipto Mangunkusumo Hospital/Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro No.71, Jakarta, Indonesia |
Background: Sepsis is still leading cause of death in critically ill children. Early recognition of sepsis and treatments are needed to reduce its mortality. The use of citrullinated Histone H3 (Cit-H3) as an early sepsis marker and outcome predictor has been validated in previous studies among adults. However, only one study in pediatric meningococcal sepsis was reported with contradictory results. This study aims to determine Cit-H3 levels in pediatric clinical sepsis and analyze its association with sepsis severity and survival rate. METHODS: A prospective observational cohort study involving 67 pediatric subjects clinically diagnosed sepsis was conducted. Cit-H3 levels, Pediatric Logistic Organ Dysfunction-2 (PELOD-2) score, and Pediatric Sequential Organ Failure Assessment (pSOFA) score were assessed at the time of diagnosis (0-hour) and 48 hours later. Pearson Correlation test was used to determine the correlation between Cit-H3 levels with PELOD-2 andpSOFA scores and receiver operating curve to find the cutoff of Cit-H3 levels on clinical sepsis patients. RESULTS: Among clinically sepsis patients, the median Cit-H3 level was 1,210 (800-32,160) ng/mL, with optimal cut-off point ≥1200 ng/mL (sensitivity 83.3% and specificity 75.7%) to discriminate sepsis. The median Cit-H3 levels at 0-hour were lower in survivor compared to non-survivor group (p=0.016). Cit-H3 level was able to predict mortality with optimal cut-off point ≥1,200 ng/mL, sensitivity 72.2% and specificity 57.1% (AUC of 69.2%; p=0.017). Using survival analysis, Cit-H3 was significantly associated with the mortality rate (p=0.023; hazard ratio of 3.45). CONCLUSION: Cit-H3 level could be potential to predict pediatric sepsis events and its outcome. © 2021 |
citrullinated histone H3; neutrophil extracellular traps; pediatric sepsis; PELOD-2 score; pSOFA score; survival |
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Prodia Education and Research Institute |
20853297 |
|
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Article |
Q4 |
160 |
22228 |
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689 |
Kartika R.W., Alwi I., Suyatna F.D., Sandra F., Yunir E., Waspadji S., Immanuel S., Silalahi T., Sungkar S., Rachmat J., Bardosono S., Reksodiputro M.H. |
57223447932;15055173800;56039633100;56016164200;36520254800;8678136400;57272979000;56275255400;57016857300;6507225348;21933841000;35090488800; |
Hyaluronic Acid Accelerates VEGF and PDGF Release from Advance Platelet Rich Fibrin in Diabetic Foot Ulcer |
2021 |
Indonesian Biomedical Journal |
13 |
3 |
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332 |
336 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117336402&doi=10.18585%2finabj.v13i3.1523&partnerID=40&md5=8bff3d535a0c8b7bbd27fc12d2903eb5 |
Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Department of Internal Medicine, Faculty of Medicine, Krida Wacana Christian University, Jl. Tanjung Duren Raya No.4, Jakarta, Indonesia; Department of Clinical Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Facial Plastic Reconstructive Division, Department of Otorhinolaryngology, Faculty of Medicine, Universitas Indonesia –Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia |
Kartika, R.W., Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia, Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Alwi, I., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Suyatna, F.D., Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Sandra, F., Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta, Indonesia; Yunir, E., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Waspadji, S., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Immanuel, S., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia; Silalahi, T., Department of Internal Medicine, Faculty of Medicine, Krida Wacana Christian University, Jl. Tanjung Duren Raya No.4, Jakarta, Indonesia; Sungkar, S., Department of Clinical Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Rachmat, J., Department of Thoracic, Cardiac and Vascular Surgery, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Bardosono, S., Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Reksodiputro, M.H., Facial Plastic Reconstructive Division, Department of Otorhinolaryngology, Faculty of Medicine, Universitas Indonesia –Cipto Mangunkusumo Hospital, Jl. Pangeran Diponegoro No.71, Jakarta, Indonesia |
Background: Hyaluronic acid (HA) is an essential component of extracellular matrix and mediates signaling in wound healing. HA could induce growth factor release from Advanced Platelet Rich Fibrin (A-PRF), including Vascular Endothelial Growth Factor (VEGF) and Platelet-derived Growth Factor (PDGF). However, concentrations of the released-VEGF and PDGF have not been clearly disclosed. Therefore, current study was conducted to measure the release of these growth factors in HA + A-PRF gel of diabetic foot ulcer (DFU) subjects. Methods: Twenty DFU subjects were included in the study and treated with A-PRF or HA+A-PRF. A-PRF was derived from autologous peripheral blood and processed with low-speed centrifugation. HA was added with a ratio of 1:0.6. A-PRF or HA + A-PRF was applied topically on DFU. Upper tips of A-PRF or HA + A-PRF gels were collected on day 0, 3 and 7 for measurements of VEGF and PDGF concentrations with Enzyme-linked Immune-sorbent Assay (ELISA) methods. Results: On day-3, both VEGF and PDGF concentrations of HA + A-PRF group were significantly higher than the VEGF (p=0.000) and PDGF (p=0.019) concentrations of A-PRF group. The VEGF and PDGF concentrations were continuously and significantly increased on day-7 of HA + A-PRF group, compared to the VEGF (p=0.000) and PDGF (p=0.004) concentrations of A-PRF group. Conclusion: Combination HA+A-PRF induces VEGF and PDGF release from A-PRF. A mixture of A-PRF and HA could be more effective than A-PRF alone for treatment of DFU. © 2021. The Indonesian Biomedical Journal. All Rights Reserved. |
advanced platelet rich fibrin; diabetic foot ulcer; growth factor; hyaluronic acid; PDGF; PRF; VEGF |
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Prodia Education and Research Institute |
20853297 |
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Article |
Q4 |
160 |
22228 |
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713 |
Mulyadi R., Hatta M., Islam A.A., Murtala B., Tammase J., Firdaus M., Susanto E., Prihartono J. |
56403164500;8134403900;55504893500;57216630400;57216632154;57220366722;57204423225;6602605635; |
Intratumoral and Peritumoral Apparent Diffusion Coefficient and MGMT mRNA Expression in Different Meningioma Histopathological Grade |
2021 |
Indonesian Biomedical Journal |
13 |
1 |
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97 |
105 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115108397&doi=10.18585%2finabj.v13i1.1338&partnerID=40&md5=22848104129d76ff75e6024180be5997 |
Neuroradiology Division, Department of Radiology, Faculty of Medicine, Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo, Jl. Diponegoro No. 71, Jakarta, 10430, Indonesia; Molecular Biology and Immunology Laboratory, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Department of Neurosurgery, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Department of Radiology, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Department of Neurology, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Department of Neurosurgery, Dharmais Hospital National Cancer Center, Jl. Letjen Jend. S. Parman No.84-86, Jakarta, 11420, Indonesia; Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo, Jl. Diponegoro No. 71, Jakarta, 10430, Indonesia; Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo, Jl. Diponegoro No. 71, Jakarta, 10430, Indonesia |
Mulyadi, R., Neuroradiology Division, Department of Radiology, Faculty of Medicine, Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo, Jl. Diponegoro No. 71, Jakarta, 10430, Indonesia; Hatta, M., Molecular Biology and Immunology Laboratory, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Islam, A.A., Department of Neurosurgery, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Murtala, B., Department of Radiology, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Tammase, J., Department of Neurology, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia; Firdaus, M., Department of Neurosurgery, Dharmais Hospital National Cancer Center, Jl. Letjen Jend. S. Parman No.84-86, Jakarta, 11420, Indonesia; Susanto, E., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo, Jl. Diponegoro No. 71, Jakarta, 10430, Indonesia; Prihartono, J., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo, Jl. Diponegoro No. 71, Jakarta, 10430, Indonesia |
BACKGROUND: Histopathological examination is the gold standard for diagnosing meningioma and determining the treatments. However, it is invasive in nature. This study was conducted to identify intratumoral and peritumoral apparent diffusion coefficient (ADC) value and mRNA O6-methylguanine-DNA methyltransferase (MGMT) expression in meningioma. METHODS: Data were collected from 39 patients who were clinically diagnosed with meningioma. However, only 37 patients met the inclusion criteria. These subjects then underwent examinations and received treatment from October 2017 to September 2018. Magnetic resonance imaging (MRI) data with diffusion-weighted imaging-apparent diffusion coefficient (DWI-ADC) sequence, histopathological diagnosis of meningioma, and results of MGMT mRNA expression were obtained. RESULTS: The most frequent type of low-grade and overall tumor was meningioma not otherwise specified (56.8%). For high-grade tumor, there were two atypical cases: atypical meningioma (2.7%) and rhabdoid meningioma (2.7%). Meningothelial meningioma had the highest mean value of minimum intratumoral ADC at 864.57±219 x10-3 mm2/s, whereas rhabdoid meningioma had the lowest at 417 x10-3 mm2/s. For minimum peritumoral ADC, rhabdoid meningioma had the highest mean value at 1,651 x10-3 mm2/s, while atypical meningioma has the lowest at 1,281 x10-3 mm2/s. For MGMT mRNA, meningothelial meningioma had the highest mean value at 10±1.2 fold change, whereas rhabdoid meningioma had the lowest mean at 6.18 fold change. CONCLUSION: WHO grade I meningiomas had higher minimum intratumoral ADC values and higher MGMT mRNA expression than the high-grade tumors. Minimum peritumoral ADC values differed across the histopathological grades. © 2021. All Rights Reserved. |
ADC; meningioma; messenger; methyltransferases; MGMT mRNA; MRI; RNA; RT-PCR |
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Prodia Education and Research Institute |
20853297 |
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|
Article |
Q4 |
160 |
22228 |
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779 |
Kartika R.W., Alwi I., Suyatna F.D., Yunir E., Waspadji S., Immanuel S., Silalahi T., Sungkar S., Rachmat J., Bardosono S., Reksodiputro M.H. |
57223447932;15055173800;56039633100;36520254800;8678136400;12777341300;56275255400;57016857300;6507225348;21933841000;35090488800; |
Advance-Platelet Rich Fibrin and Hyaluronic Acid Combination Improves Interleukin-6 and Granulation Index in Diabetic Foot Ulcer Patients |
2021 |
Indonesian Biomedical Journal |
13 |
2 |
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170 |
177 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108646812&doi=10.18585%2finabj.v13i2.1501&partnerID=40&md5=38a4d6d4cd5994b04badcabc1939a76e |
Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Thoracic, Cardiac and Vascular Surgery, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Internal Medicine, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Department of Clinical Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Thoracic Cardiac and Vascular Surgery, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Department of Nutrition, Faculty of Medicine Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Facial Plastic Reconstructive Division, Department of Otorhinolaryngology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia |
Kartika, R.W., Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia, Department of Thoracic, Cardiac and Vascular Surgery, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Alwi, I., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia; Suyatna, F.D., Department of Clinical Pharmacology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Yunir, E., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia; Waspadji, S., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia; Immanuel, S., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia; Silalahi, T., Department of Internal Medicine, Krida Wacana Christian University, Jl. Arjuna Utara No.6, Jakarta, Indonesia; Sungkar, S., Department of Clinical Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Rachmat, J., Department of Thoracic Cardiac and Vascular Surgery, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Bardosono, S., Department of Nutrition, Faculty of Medicine Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, Indonesia; Reksodiputro, M.H., Facial Plastic Reconstructive Division, Department of Otorhinolaryngology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jl. Salemba Raya No.6, Jakarta, Indonesia |
BACKGROUND: Diabetic foot ulcer (DFU) is the most common and threatening complication of Diabetes Mellitus (DM). Ideal wound dressing for DFU management should relieve symptoms, provide wound protection, and encourage healing. Advanced-Platelet Rich Fibrin (A-PRF) and Hyaluronic Acid (HA) have been proven to improve wound healing process. This study was aimed to demonstrate the ability of combination of A-PRF and HA in reducing inflammation and improving DFU tissue healing. METHODS: Twenty DFU subjects were involved in this study, and divided into two groups based on the topical fibrin gel treatment: A-PRF + HA group and A-PRF only group. A-PRF was obtained by peripheral blood centrifugation. A-PRF + HA was prepared by homogening A-PRF and AH with a ratio of 1:0.6. Interleukin-6 (IL-6) level, granulation index (GI), numeric pain score (NPS), and inflammation clinical symptoms (ICS) were assessed on day-0, 3, 7 and 14. RESULTS: Wound swabs' IL-6 level on day-7 was found to be significantly lower in A-PRF + HA compared to A-PRF alone (p=0.041). The IL-6 level reduction also found to be significant higher either in wound swabs (day 0-7, p=0.015) or fibrin gel (day 0-3, p=0.049; day 0-7, p=0.034). A-PRF + HA treatment significantly increased the GI even since day-3 (p=0.043), with lower NPS (p<0.001), and ICS score. CONCLUSION: The combination of A-PRF and HA increases the GI in DFU healing by reducing the inflammation state which will induce the angiogenesis process, as well as reducing pain in DFU subjects better than A-PRF alone. © 2021. All Rights Reserved. |
angiogenesis; inflammation; interleukin-6; proliferation; wound healing |
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Prodia Education and Research Institute |
20853297 |
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Article |
Q4 |
160 |
22228 |
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