528 |
Kusmardi K., Wiyarta E., Estuningtyas A., Sahar N., Midoen Y.H., Tedjo A., Pakpahan A. |
56966625300;57221521342;55650360200;57212464367;57197805109;57189320451;57200109636; |
Potential inhibition by Phaleria macrocarpa leaves ethanol extract on Ki-67 expression in distal colon mouse |
2021 |
Pharmacognosy Journal |
13 |
2 |
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443 |
449 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103626996&doi=10.5530%2fpj.2021.13.56&partnerID=40&md5=0fdcd0a987696734078bf217c05eefb5 |
Department of Anatomic Pathology, Drug Development Research Cluster, Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa, Jakarta, Indonesia |
Kusmardi, K., Department of Anatomic Pathology, Drug Development Research Cluster, Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Wiyarta, E., Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Estuningtyas, A., Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Sahar, N., Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Midoen, Y.H., Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Tedjo, A., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Pakpahan, A., Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa, Jakarta, Indonesia |
Ulcerative colitis (UC) has been an important aspect of an incurable chronic inflammatory disease over the last few decades. To find useful therapies for UC, one of which is herbal therapy, many researches have been conducted. Due to its anti-inflammatory effects, Phaleria macrocarpa (PM), an Indonesian indigenous herb, is considered to be the alternative therapy for UC. Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is then used in this research to determine its effect on UC by using Ki-67 as a marker of proliferation. PMLEE was created from dry PM content undergoing maceration. The animals were classified into six categories: normal, positive control, negative control and PMLEE group (100, 200, 300 mg/kgBW). PMLEE was then injected for 7 consecutive days into BALB/c mice that were caused by dextran sodium sulphate (DSS). DSS is used for modeling UC in the colon tissue of mice. All mice were terminated and then stained with anti-Ki-67 after their colons were extracted. Subsequently, the stained parts were analyzed with ImageJ based on the color intensity produced by the results of H-score. Based on H-score, PMLEE 300mg and 200mg has significantly decreased the expression of Ki-67 compare to the negative control (p=0.001 and p=0.01). PMLEE also has a tendency to be dose dependent based on the significant difference from PMLEE 300mg and 100mg (p=0.002). It then concludes that PMLEE is related to Ki-67 expression in cells, as it was inversely proportional in this analysis. © 2021 Phcogj.Com. |
Dextran sodium sulphate; Inflamation; Ki-67; Mahkota Dewa (Phaleria macrocarpa) |
acetylsalicylic acid; alcohol; antiinflammatory agent; dextran sulfate; Ki 67 antigen; Phaleria macrocarpa extract; plant extract; unclassified drug; animal experiment; animal model; animal tissue; antigen expression; antiinflammatory activity; Article; colon tissue; controlled study; data analysis software; descending colon; dysplasia; medicinal plant; mouse; nonhuman; Phaleria macrocarpa; plant leaf; proliferation index; scoring system; treatment duration; ulcerative colitis |
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