649 |
Mukti A.I., Ilyas S., Warli S.M., Putra A., Rasyid N., Munir D., Siregar K.B., Ichwan M., Alif I., Hidayah N. |
57376899500;55980597400;57189610001;57197818079;56245069300;57039203600;57190863373;26040779000;57215218352;57377826800; |
Mesenchymal stem cells enhance vascular endothelial growth factor-A, endothelial nitric oxide synthetase, and HSP70 expression in improving erectile dysfunction in streptozotocin-induced diabetic rats |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
|
|
1174 |
1180 |
|
|
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121417031&doi=10.3889%2foamjms.2021.7801&partnerID=40&md5=d2741187724b6c10a2d9b17fcabd3aad |
Department of Doctoral Degree Program, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia; Department of Urology, Faculty of Medicine, Universitas Sumatera Utara Hospital, Medan, Indonesia; Stem Cell and Cancer Research, Faculty of Medical, Sultan Agung Islamic University, Semarang, Indonesia; Department of Pathology, Medical Faculty, Sultan Agung Islamic University, Semarang, Indonesia; Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University, Semarang, Indonesia; Department of Urology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Pusat Unggulan IPTEK Tissue Engineering, Universitas Sumatera Utara, Medan, Indonesia; Department of Oncology Surgery, Faculty of Medicine, Universitas Sumatera Utara, Universitas Sumatera Utara Hospital, Medan, Indonesia; Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia |
Mukti, A.I., Department of Doctoral Degree Program, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Ilyas, S., Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia; Warli, S.M., Department of Urology, Faculty of Medicine, Universitas Sumatera Utara Hospital, Medan, Indonesia; Putra, A., Stem Cell and Cancer Research, Faculty of Medical, Sultan Agung Islamic University, Semarang, Indonesia, Department of Pathology, Medical Faculty, Sultan Agung Islamic University, Semarang, Indonesia, Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University, Semarang, Indonesia; Rasyid, N., Department of Urology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Munir, D., Department of Doctoral Degree Program, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia, Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia, Pusat Unggulan IPTEK Tissue Engineering, Universitas Sumatera Utara, Medan, Indonesia; Siregar, K.B., Department of Oncology Surgery, Faculty of Medicine, Universitas Sumatera Utara, Universitas Sumatera Utara Hospital, Medan, Indonesia; Ichwan, M., Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Alif, I., Stem Cell and Cancer Research, Faculty of Medical, Sultan Agung Islamic University, Semarang, Indonesia; Hidayah, N., Stem Cell and Cancer Research, Faculty of Medical, Sultan Agung Islamic University, Semarang, Indonesia |
AIM: This study investigated the therapeutic role of mesenchymal stem cells (MSCs) on erectile function in a diabetes mellitus erectile dysfunction (DMED) rat model by analyzing the expression of endothelial nitric oxide synthetase (eNOS), vascular endothelial growth factor A (VEGF-A), and the 70 kilodalton heat shock proteins (HSP70). METHODS: MSCs were isolated from umbilical cords (UCs), and their characteristics identified by flow cytometry and osteogenic differentiation analysis. Thirty 8-week-old rats were divided into four groups: Sham, control, T1, and T2. After a 16 h fast, 24 rats were randomly selected and intraperitoneally injected with streptozotocin (STZ) to induce diabetes mellitus. At 8 weeks after STZ injection, rats with DMED were classified into four groups, sham, control group (DMED rats received 500 μL phosphate buffer saline [PBS]); T1 (DMED rats treated with 500 μL PBS containing 1 × 106 UC-MSCs); and T2 (DMED rats treated with 500 μL PBS containing 2 × 106 UC-MSCs). Eight weeks after MSCs administration, the rats’ erectile function was measured by cavernous nerve stimulation. The blinded histological and gene expression assessment were used to analyze the eNOS, HSP70 content, and VEGF-A expression on the penile tissues. RESULTS: MSCs administration, rats in T1 and T2 groups showed a significant enhancement of erectile response that showed a trend of increase of VEGF-A mRNA level expression was 2.2 ± 0.61 in T2 Group supported with the optimum recovery of eNOS, in which the value of eNOS expression was 20.66% ± 2.32%. While optimum decrease of HSP70 content, the value of HSP70 expression was 15.50% ± 0.90%. IHC results showed that the DMED induction in rats caused a significant decrease of eNOS content in corpus cavernosum tissue. CONCLUSION: MSCs could ameliorate DMED in rats by increasing VEGF-A and decreasing HSP70 and eNOS, indicating these cells offer a potential application for DMED patients’ treatment. © 2021 Ade Indra Mukti, Syafruddin Ilyas, Syah Mirsya Warli, Agung Putra, Nur Rasyid, Delfitri Munir, Kamal Basri Siregar, Muhammad Ichwan, Iffan Alif, Nurul Hidayah. |
Endothelial nitric oxide synthetase; Erectile dysfunction; HSP70; Mesenchymal stem cells; Vascular endothelial growth factor A-A |
heat shock protein; heat shock protein 70; messenger RNA; nitric oxide synthase; vasculotropin A; animal cell; animal experiment; animal model; animal tissue; Article; cell differentiation; controlled study; corpus cavernosum; erectile dysfunction; female; flow cytometry; gene expression; glucose blood level; histology; hypoglycemia; immunohistochemistry; International Index of Erectile Function; lipid storage; male; mesenchymal stem cell; nerve stimulation; nonhuman; polymerase chain reaction; protein expression; rat; real time polymerase chain reaction; Sertoli cell; streptozotocin-induced diabetes mellitus |
Scientific Foundation SPIROSKI |
18579655 |
|
|
Article |
Q3 |
288 |
15252 |
|
|