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8 |
Sartika D., Nurrachmah E., Sukirman D.I., Mansyur M., Supartono B. |
57467276300;57466703100;57467568600;37085506800;57205286040; |
Ergonomic Risk-prone Activities toward Nurses in the Intensive Care and Emergency Room |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
T5 |
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48 |
53 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125269929&doi=10.3889%2fOAMJMS.2021.7851&partnerID=40&md5=e4f606505a7f1d2f7fa6498e532fb934 |
Health Polytechnic of Riau, Pekanbaru, Indonesia; Department of Medical Surgery, Faculty of Nursing, University of Indonesia, Jakarta, Indonesia; Department of Basic Science and Fundamentals of Nursing, Faculty of Nursing, University of Indonesia, Jakarta, Indonesia; Department of Community Medicine, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Faculty of Medicine, Pembangunan Nasional University, Jakarta, Indonesia |
Sartika, D., Health Polytechnic of Riau, Pekanbaru, Indonesia; Nurrachmah, E., Department of Medical Surgery, Faculty of Nursing, University of Indonesia, Jakarta, Indonesia; Sukirman, D.I., Department of Basic Science and Fundamentals of Nursing, Faculty of Nursing, University of Indonesia, Jakarta, Indonesia; Mansyur, M., Department of Community Medicine, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Supartono, B., Faculty of Medicine, Pembangunan Nasional University, Jakarta, Indonesia |
BACKGROUND: Nurses have the risk of ergonomic hazards in providing nursing care, especially with increasingly dynamic health services such as during Coronavirus disease-19 pandemic like today. AIM: The aim of the study was to evaluate activities prone to produce ergonomic risks during the implementation of nursing care in intensive care and emergency room (ER) of a hospital in Riau, Indonesia. METHODOLOGY: This study was conducted by observing the routine activities conducted by the nurses and using similar task group techniques equipped with Rapid Entire Body Assessment instrument. Those observed activities were obtained from 17 intensive care room nurses and ten ER nurses. There were six activities observed in the intensive care room: Bathing, transferring the patient, wounds dressing, taking blood samples for the AGDA examinations, as well as inserting the intravenous needle and electrocardiograms. Meanwhile, there were two activities observed in the ER: Transferring the patient and inserting the intravenous needle. RESULTS: The highest ergonomic risks activity in the intensive care room was bathing the patient with a total score of 13. At the ER, the highest risk score was transferring the patient with a total score of 12. Both activities were at level 4, indicating a high-risk condition. Thus, examinations and changes should be immediately initiated. CONCLUSION: The results are significant to be paid attention by the related parties at the hospital to facilitate some improvements immediately. In addition, the ergonomic approaches that can be suggested to the nurses are regular stretching, physical exercises, and applying ergonomic principles while working. © 2021 Dewi Sartika, Elly Nurrachmah, Dewi Irawaty Sukirman, Muchtaruddin Mansyur, Basuki Supartono. |
Emergency room; Ergonomic risk; Intensive care; Nurse’s activity; Nursing intervention |
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Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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34 |
Gustiananda M., Sulistyo B.P., Agustriawan D., Andarini S. |
6507570171;57215020738;55382929300;8716259500; |
Immunoinformatics analysis of sars-cov-2 orf1ab polyproteins to identify promiscuous and highly conserved t-cell epitopes to formulate vaccine for indonesia and the world population |
2021 |
Vaccines |
9 |
12 |
1459 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121528747&doi=10.3390%2fvaccines9121459&partnerID=40&md5=e6eaaf1e02bbe5e81bf5d0e390d38566 |
Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, University of Indonesia, Persahabatan Hospital, Jl Persahabatan Raya 1, Jakarta, 13230, Indonesia |
Gustiananda, M., Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Sulistyo, B.P., Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Agustriawan, D., Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Andarini, S., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, University of Indonesia, Persahabatan Hospital, Jl Persahabatan Raya 1, Jakarta, 13230, Indonesia |
SARS-CoV-2 and its variants caused the COVID-19 pandemic. Vaccines that target conserved regions of SARS-CoV-2 and stimulate protective T-cell responses are important for reducing symptoms and limiting the infection. Seven cytotoxic (CTL) and five helper T-cells (HTL) epitopes from ORF1ab were identified using NetCTLpan and NetMHCIIpan algorithms, respectively. These epitopes were generated from ORF1ab regions that are evolutionary stable as reflected by zero Shannon’s entropy and are presented by 56 human leukocyte antigen (HLA) Class I and 22 HLA Class II, ensuring good coverage for the Indonesian and world population. Having fulfilled other criteria such as immunogenicity, IFNγ inducing ability, and non-homology to human and microbiome peptides, the epitopes were assembled into a vaccine construct (VC) together with β-defensin as adjuvant and appropriate linkers. The VC was shown to have good physicochemical characteristics and capability of inducing CTL as well as HTL responses, which stem from the engagement of the vaccine with toll-like receptor 4 (TLR4) as revealed by docking simulations. The most promiscuous peptide899WSMATYYLF907 was shown via docking simulation to interact well with HLA-A*24:07, the most predominant allele in Indonesia. The data presented here will contribute to the in vitro study of T-cell epitope mapping and vaccine design in Indonesia. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
Cytotoxic T-cells; Helper T-cells; HLA-A*24:07; Human leukocyte antigen; Immunoinformatics; Multi-epitope peptide-based vaccine; SARS-CoV-2; T-cell epitopes |
epitope; gamma interferon; HLA A antigen; HLA antibody; T lymphocyte receptor; toll like receptor 4; allele; allergenicity; amino acid sequence; antigenicity; Article; binding affinity; CD8+ T lymphocyte; controlled study; cytotoxic T lymphocyte; endoplasmic reticulum; entropy; epitope mapping; gene frequency; gene structure; HLA typing; human; human cell; hydrophilicity; immune response; immunogenicity; immunoinformatics; Indonesia; microbiome; molecular docking; open reading frame; peptide synthesis; protein interaction; protein secondary structure; protein structure; sequence alignment; sequence analysis; sequence homology; Severe acute respiratory syndrome coronavirus 2; vaccination |
MDPI |
2076393X |
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Article |
Q1 |
1296 |
2913 |
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84 |
Makmun D., Simadibrata M., Abdullah M., Syam A.F., Shatri H., Fauzi A., Renaldi K., Maulahela H., Utari A.P., Pribadi R.R., Muzellina V.N., Nursyirwan S.A. |
16638046900;23499598400;7103393434;8443384400;28767986500;36518523000;57190963547;57189612709;57113585900;57194732282;57223968527;57249143800; |
Retrospective Study Colorectal cancer patients in a tertiary hospital in Indonesia: Prevalence of the younger population and associated factors |
2021 |
World Journal of Clinical Cases |
9 |
32 |
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9804 |
9814 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119652838&doi=10.12998%2fwjcc.v9.i32.9804&partnerID=40&md5=75cf4743775cd03169188a4206cb7cb0 |
Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Clinical Epidemiology Unit, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia |
Makmun, D., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Simadibrata, M., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Abdullah, M., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Syam, A.F., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Shatri, H., Clinical Epidemiology Unit, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Fauzi, A., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Renaldi, K., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Maulahela, H., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Utari, A.P., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Pribadi, R.R., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Muzellina, V.N., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia; Nursyirwan, S.A., Division of Gastroenterology, Pancreatobiliary & Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesiao, Cipto Mangunkusumo National General Hospital, Jakarta, 10430, Indonesia |
BACKGROUND An increasing trend in colorectal cancer (CRC) occurring at younger ages has been observed worldwide, even though incidence is declining in the general population. Most currently available guidelines still recommend CRC screening for older populations, despite an alarming rise in early-onset CRC incidence. Risk stratification is necessary to further determine the population most at risk for early-onset CRC. However, epidemiological data on related clinical characteristics and potential risk factors, especially in developing countries, have not been widely reported. AIM To investigate the prevalence, demographics, clinicopathologic features, and associated factors of young-onset CRC patients in a tertiary hospital in Indonesia. METHODS Patients undergoing colonoscopy examination between 2008 and 2019, yielding a diagnosis of CRC were identified from medical records. The subjects were classified into two groups according to their age at diagnosis, namely early-onset (18–49 years old) and late-onset (≥ 50-years-old). Demographic data, characteristics, and risk factors of both onset age groups were evaluated using the chisquare and Fisher’s exact test. RESULTS Among 495 CRC patients confirmed by histopathology, 205 (41.4%) were classified as early-onset and 290 (58.6%) as late-onset. Most subjects in the early-onset CRC group were male (53.7%), with 89.8% displaying adenocarcinoma histopathology. A majority (78%) of the early-onset CRC patients had left-sided tumors, with the rectum (41%) and rectosigmoid (17.6%) being the most common sites. Abdominal pain was the most frequent symptom in the early-onset CRC patients (55.6%), which was significantly higher than that in the late-onset CRC patients (43.8%, P > 0.05). Early-onset CRC cases were more likely to be underweight (34.6% vs 20.0%, P < 0.001) compared to late-onset CRC cases. The proportion of subjects with suspected hereditary nonpolyposis colorectal cancer (HNPCC) was also higher in the early-onset CRC group than in the late-onset age group (9.3% vs 4.1%, P < 0.05). However, no difference was observed in the parental or family histories of CRC cases. CONCLUSION Early-onset CRC patients were more likely to have abdominal pain, underweight status, and HNPCC suspicion than late-onset CRC patients. © The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. |
Associated factors; Colorectal cancer; Early onset; Epidemiology; Indonesia; Tertiary hospital |
abdominal pain; adenocarcinoma; adult; aged; Article; body mass; carcinoid; colon; colonoscopy; colorectal cancer; cross-sectional study; diabetes mellitus; familial adenomatous polyposis; female; hereditary nonpolyposis colorectal cancer; histopathology; human; human tissue; hypertension; major clinical study; male; medical record; obesity; prevalence; rectum; rectum hemorrhage; retrospective study; risk factor; smoking; underweight; very elderly |
Baishideng Publishing Group Co |
23078960 |
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Article |
Q3 |
368 |
12908 |
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88 |
Lokeswara A.W., Hiksas R., Irwinda R., Wibowo N. |
57200937543;57226152029;57205713130;15049026900; |
Preeclampsia: From Cellular Wellness to Inappropriate Cell Death, and the Roles of Nutrition |
2021 |
Frontiers in Cell and Developmental Biology |
9 |
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726513 |
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2 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119532670&doi=10.3389%2ffcell.2021.726513&partnerID=40&md5=38456382efeb541572619e5bed470318 |
Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia; Maternal Fetal Division, Department of Obstetrics and Gynaecology, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia |
Lokeswara, A.W., Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia; Hiksas, R., Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia; Irwinda, R., Maternal Fetal Division, Department of Obstetrics and Gynaecology, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia; Wibowo, N., Maternal Fetal Division, Department of Obstetrics and Gynaecology, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia |
Preeclampsia is one of the most common obstetrical complications worldwide. The pathomechanism of this disease begins with abnormal placentation in early pregnancy, which is associated with inappropriate decidualization, vasculogenesis, angiogenesis, and spiral artery remodeling, leading to endothelial dysfunction. In these processes, appropriate cellular deaths have been proposed to play a pivotal role, including apoptosis and autophagy. The proper functioning of these physiological cell deaths for placentation depends on the wellbeing of the trophoblasts, affected by the structural and functional integrity of each cellular component including the cell membrane, mitochondria, endoplasmic reticulum, genetics, and epigenetics. This cellular wellness, which includes optimal cellular integrity and function, is heavily influenced by nutritional adequacy. In contrast, nutritional deficiencies may result in the alteration of plasma membrane, mitochondrial dysfunction, endoplasmic reticulum stress, and changes in gene expression, DNA methylation, and miRNA expression, as well as weakened defense against environmental contaminants, hence inducing a series of inappropriate cellular deaths such as abnormal apoptosis and necrosis, and autophagy dysfunction and resulting in abnormal trophoblast invasion. Despite their inherent connection, the currently available studies examined the functions of each organelle, the cellular death mechanisms and the nutrition involved, both physiologically in the placenta and in preeclampsia, separately. Therefore, this review aims to comprehensively discuss the relationship between each organelle in maintaining the physiological cell death mechanisms and the nutrition involved, and the interconnection between the disruptions in the cellular organelles and inappropriate cell death mechanisms, resulting in poor trophoblast invasion and differentiation, as seen in preeclampsia. Copyright © 2021 Lokeswara, Hiksas, Irwinda and Wibowo. |
apoptosis; autophagy; cell death; cellular wellness; nutrition; preeclampsia |
aneuploidy; cell death; cell differentiation; cell invasion; cell membrane; cell organelle; endoplasmic reticulum; endoplasmic reticulum stress; environmental factor; epigenetics; gene expression; human; mitochondrion; nonhuman; nutrition; preeclampsia; pregnancy; Review; trophoblast; vascular remodeling |
Frontiers Media S.A. |
2296634X |
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Review |
Q1 |
2452 |
946 |
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136 |
Daulay R.S., Saragih R.A.C., Daulay R.M., Ganie R.A., Tann G., Supriyatno B. |
57201677329;57204321772;6504644320;57193788722;57213061716;37068046400; |
Role of interferon-gamma +874 a/t single-nucleotide polymorphism and tuberculosis susceptibility of pediatric population in north sumatera, indonesia |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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1057 |
1060 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121721756&doi=10.3889%2foamjms.2021.7441&partnerID=40&md5=490f692392638585ec48409e7140509b |
Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Daulay, R.S., Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Saragih, R.A.C., Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Daulay, R.M., Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Ganie, R.A., Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Tann, G., Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Supriyatno, B., Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
BACKGROUND: Tuberculosis (TB) remains to be a leading cause of morbidity and mortality worldwide. The immune defense against Mycobacterium tuberculosis is complicated. Interferon-gamma (IFN-γ) is the main cytokine involved in the immune response of TB. To date, the role of +874 A/T single-nucleotide polymorphism (SNP) and TB disease susceptibility continues to be controversial. AIM: The aim of this study was to investigate the role of +874 A/T SNP and TB disease susceptibility of pediatric population in North Sumatera, Indonesia. METHODS: A case–control study was conducted in Medan and Batubara, North Sumatera, Indonesia, from January to December 2016. A total of 51 children with TB and 51 healthy controls were enrolled in this study. Subjects were 2 months–14 years old age children diagnosed with TB and written informed consent from the parents or the caregivers to participate. Subjects were withdrawn from the study when immunodeficiency condition was found or suffered from other infection disease. DNA samples were obtained from all of the subjects. +874 A/T SNP was identified by performing the amplification refractory mutational system-polymerase chain reaction method. IFN-γ levels were measured using human enzyme-linked immunosorbent assay. Data analysis was performed using Chi-square and Mann–Whitney test. P < 0.05 was considered statistically significant. RESULTS: The result of this study reveals that the presence of AA, AT, and TT genotype in TB patients was 31 (60.8%), 20 (39.2%), and 0 (0%), respectively (p = 0.023). Significant decreased production of IFN-γ levels (p = 0.042) was found in TB patients 9.41 (1.10–28.06) pg/ml. CONCLUSION: Our study demonstrated significant evidence of the role of +874 A/T SNP and TB disease susceptibility of pediatric population in North Sumatera, Indonesia, predominantly AA genotype. Significant decreased production of IFN-γ reported among pediatric TB. © 2021 Rini Savitri Daulay, Rina Amalia C. Saragih, Ridwan Muchtar Daulay, Ratna Akbari Ganie, Gino Tann, Bambang Supriyatno. |
+874 A/T; Indonesia; Interferon-gamma; Pediatric; Single-nucleotide polymorphism; Tuberculosis |
gamma interferon; adolescent; amplification refractory mutation system polymerase chain reaction; Article; case control study; child; controlled study; cytokine production; disease predisposition; DNA isolation; enzyme linked immunosorbent assay; female; fine needle aspiration biopsy; gene frequency; genotype; human; immune deficiency; infant; major clinical study; male; newborn; preschool child; school child; single nucleotide polymorphism; thorax radiography; tuberculin test; tuberculosis |
Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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140 |
Waters S., Agostino M., Lee S., Ariyanto I., Kresoje N., Leary S., Munyard K., Gaudieri S., Gaff J., Irish A., Keil A.D., Price P., Allcock R.J.N. |
57195514207;34771068500;56272877300;57193538110;55413004800;56906725500;6506851062;56245970200;57193534280;7004314741;57339623900;57201814264;7003764659; |
Sequencing directly from clinical specimens reveals genetic variations in HCMV-encoded chemokine receptor us28 that may influence antibody levels and interactions with human chemokines |
2021 |
Microbiology Spectrum |
9 |
2 |
e00020-21 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119150219&doi=10.1128%2fSpectrum.00020-21&partnerID=40&md5=d943d30d66f9a7de80628f87fcbb3810 |
Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Curtin Institute for Computation, Curtin University, Bentley, WA, Australia; Department of Microbiology, PathWest Laboratory Medicine WA, Nedlands, WA, Australia; Virology and Cancer Pathobiology Research Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; School of Biomedical Sciences, University of Western Australia, Perth, WA, Australia; Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, WA, Australia; School of Human Sciences, University of Western Australia, Perth, WA, Australia; Department of Medicine, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, United States; Department of Nephrology, Fiona Stanley Hospital, Murdoch, WA, Australia; Department of Diagnostic Genomics, PathWest Laboratory Medicine WA, Nedlands, WA, Australia |
Waters, S., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Agostino, M., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia, Curtin Institute for Computation, Curtin University, Bentley, WA, Australia; Lee, S., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia, Department of Microbiology, PathWest Laboratory Medicine WA, Nedlands, WA, Australia; Ariyanto, I., Virology and Cancer Pathobiology Research Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Kresoje, N., School of Biomedical Sciences, University of Western Australia, Perth, WA, Australia; Leary, S., Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, WA, Australia; Munyard, K., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Gaudieri, S., Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, WA, Australia, School of Human Sciences, University of Western Australia, Perth, WA, Australia, Department of Medicine, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, United States; Gaff, J., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Irish, A., Department of Nephrology, Fiona Stanley Hospital, Murdoch, WA, Australia; Keil, A.D., Department of Microbiology, PathWest Laboratory Medicine WA, Nedlands, WA, Australia; Price, P., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Allcock, R.J.N., School of Biomedical Sciences, University of Western Australia, Perth, WA, Australia, Department of Diagnostic Genomics, PathWest Laboratory Medicine WA, Nedlands, WA, Australia |
Human cytomegalovirus (HCMV) is a beta-herpesvirus carried by;80% of the world’s population. Acute infections are asymptomatic in healthy individuals but generate diverse syndromes in neonates, solid organ transplant recipients, and HIV-infected individuals. The HCMV gene US28 encodes a homolog of a human chemokine receptor that is able to bind several chemokines and HIV gp120. Deep sequencing technologies were used to sequence US28 directly from 60 clinical samples from Indonesian HIV patients and Australian renal transplant recipients, healthy adults, and neonates. Molecular modeling approaches were used to predict whether nine nonsynonymous mutations in US28 may alter protein binding to a panel of six chemokines and two variants of HIV gp120. Ninety-two percent of samples contained more than one variant of HCMV, as defined by at least one nonsynonymous mutation. Carriage of these variants differed between neonates and adults, Australian and Indonesian samples, and saliva samples and blood leukocytes. Two nonsynonymous mutations (N170D and R267K) were associated with increased levels of immediate early protein 1 (IE-1) and glycoprotein B (gB) HCMV-reactive antibodies, suggesting a higher viral burden. Seven of the nine mutations were predicted to alter binding of at least one ligand. Overall, HCMV variants are common in all populations and have the potential to affect US28 interactions with human chemokines and/or gp120 and alter responses to the virus. The findings relied on deep sequencing technologies applied directly to clinical samples, so the variants exist in vivo. IMPORTANCE Human cytomegalovirus (HCMV) is a common viral pathogen of solid organ transplant recipients, neonates, and HIV-infected individuals. HCMV encodes homologs of several host genes with the potential to influence viral persistence and/ or pathogenesis. Here, we present deep sequencing of an HCMV chemokine receptor homolog, US28, acquired directly from clinical specimens. Carriage of these variants differed between patient groups and was associated with different levels of circulating HCMV-reactive antibodies. These features are consistent with a role for US28 in HCMV persistence and pathogenesis. This was supported by in silico analyses of the variant sequences demonstrating altered ligand-binding profiles. The data delineate a novel approach to understanding the pathogenesis of HCMV and may impact the development of an effective vaccine. Copyright © 2021 Waters et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
Chemokine receptor; Deep sequencing; HIV patients; Human cytomegalovirus; Renal transplant recipients; US28 |
arginine; asparagine; aspartic acid; chemokine; chemokine receptor; chemokine receptor US28; glycoprotein B; glycoprotein gp 120; immediate early protein; immediate early protein 1; lysine; unclassified drug; virus antibody; chemokine; chemokine receptor; protein binding; US28 receptor, Cytomegalovirus; viral protein; virus antibody; adult; Article; Australian; blood; controlled study; genetic variation; graft recipient; human; Human cytomegalovirus; Human immunodeficiency virus infection; Indonesian; kidney transplantation; leukocyte; molecular model; mutation; newborn; nonhuman; protein protein interaction; saliva; virus load; amino acid sequence; blood; Cytomegalovirus; cytomegalovirus infection; genetic variation; genetics; high throughput sequencing; immunology; infant; isolation and |
American Society for Microbiology |
21650497 |
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34704798 |
Article |
Q1 |
2502 |
907 |
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155 |
Blom I.M., Campos L.N., El Amine Youcef Ali M., Asyura M.M.A.Z., von Metnitz D.Z., Limann B., Janušonytė E. |
57218125715;57256325100;57257173900;57256325200;57257174000;57257030900;57211896142; |
Youth versus pandemics: the role of future generations in the pandemic treaty |
2021 |
The Lancet Global Health |
9 |
10 |
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e1361 |
e1362 |
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2 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114832726&doi=10.1016%2fS2214-109X%2821%2900307-7&partnerID=40&md5=8425b6166dd6fbd88fa21d99ef4c5772 |
International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark; University of Amsterdam, Amsterdam, Netherlands; Faculty of Medical Sciences, Universidade de Pernambuco, Recife, Brazil; Faculty of Medicine, University of Constantine 3, Constantine, Algeria; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sigmund Freud University Vienna, Vienna, Austria; University of Ghana Medical School, Accra, Ghana; Faculty of Medicine, Vilnius University, Vilnius, Lithuania |
Blom, I.M., International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark, University of Amsterdam, Amsterdam, Netherlands; Campos, L.N., International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark, Faculty of Medical Sciences, Universidade de Pernambuco, Recife, Brazil; El Amine Youcef Ali, M., International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark, Faculty of Medicine, University of Constantine 3, Constantine, Algeria; Asyura, M.M.A.Z., International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; von Metnitz, D.Z., International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark, Sigmund Freud University Vienna, Vienna, Austria; Limann, B., International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark, University of Ghana Medical School, Accra, Ghana; Janušonytė, E., International Federation of Medical Students' Associations, Nørre Allé 14, Copenhagen, 2200, Denmark, Faculty of Medicine, Vilnius University, Vilnius, Lithuania |
[No abstract available] |
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awareness; government; health care organization; health care policy; human; leadership; Note; pandemic; public health; work environment; adult; disaster planning; female; global health; legislation and jurisprudence; male; organization and management; pandemic; social responsibility; World Health Organization; young adult; Adult; Disaster Planning; Female; Global Health; Humans; Male; Pandemics; Social Responsibility; World Health Organization; Young Adult |
Elsevier Ltd |
2214109X |
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34331865 |
Note |
Q1 |
7970 |
126 |
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171 |
Pudjiadi A.H., Putri N.D., Sjakti H.A., Yanuarso P.B., Gunardi H., Roeslani R.D., Pasaribu A.D., Nurmalia L.D., Sambo C.M., Ugrasena I.D.G., Soeroso S., Firman A., Muryawan H., Setyanto D.B., Citraresmi E., Effendi J.A., Habibah L., Octaviantie P.D., Utami I.N.A., Prawira Y., Kaswandani N., Alam A., Kadafi K.T., Pulungan A.B. |
18435202300;57200573842;57195720458;57205428706;56510252700;57191512707;57287717900;57288262700;57287718000;57224730593;15133254800;57215582843;57287718100;57203009929;57224512641;57287899600;57287899700;57287718200;57287718300;55455747000;57195941745;57201672408;57211313656;57192905981; |
Pediatric COVID-19: Report From Indonesian Pediatric Society Data Registry |
2021 |
Frontiers in Pediatrics |
9 |
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716898 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116558206&doi=10.3389%2ffped.2021.716898&partnerID=40&md5=8c294f39457ac77d477f3bb1d2dd9eb4 |
The Indonesian Pediatric Society, Jakarta, Indonesia; Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Department of Pediatrics, Faculty of Medicine, Dr. Soetomo Hospital, Universitas Airlangga, Surabaya, Indonesia; Department of Pediatrics, Faculty of Medicine, Hasan Sadikin Hospital, Universitas Padjajaran, Bandung, Indonesia; Department of Pediatrics, Faculty of Medicine, Dr. Kariadi Hospital, Universitas Diponegoro, Semarang, Indonesia; Department of Pediatrics, Harapan Kita Women and Children Hospital, Jakarta, Indonesia; Department of Pediatrics, Fatmawati Hospital, Jakarta, Indonesia; Department of Pediatrics, Faculty of Medicine, Dr. Saiful Anwar Hospital, Universitas Brawijaya, Malang, Indonesia |
Pudjiadi, A.H., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Putri, N.D., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Sjakti, H.A., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Yanuarso, P.B., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Gunardi, H., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Roeslani, R.D., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Pasaribu, A.D., The Indonesian Pediatric Society, Jakarta, Indonesia; Nurmalia, L.D., The Indonesian Pediatric Society, Jakarta, Indonesia; Sambo, C.M., The Indonesian Pediatric Society, Jakarta, Indonesia; Ugrasena, I.D.G., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Dr. Soetomo Hospital, Universitas Airlangga, Surabaya, Indonesia; Soeroso, S., The Indonesian Pediatric Society, Jakarta, Indonesia; Firman, A., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Hasan Sadikin Hospital, Universitas Padjajaran, Bandung, Indonesia; Muryawan, H., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Dr. Kariadi Hospital, Universitas Diponegoro, Semarang, Indonesia; Setyanto, D.B., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Citraresmi, E., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Harapan Kita Women and Children Hospital, Jakarta, Indonesia; Effendi, J.A., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Fatmawati Hospital, Jakarta, Indonesia; Habibah, L., The Indonesian Pediatric Society, Jakarta, Indonesia; Octaviantie, P.D., Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Utami, I.N.A., The Indonesian Pediatric Society, Jakarta, Indonesia; Prawira, Y., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Kaswandani, N., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia; Alam, A., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Hasan Sadikin Hospital, Universitas Padjajaran, Bandung, Indonesia; Kadafi, K.T., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Dr. Saiful Anwar Hospital, Universitas Brawijaya, Malang, Indonesia; Pulungan, A.B., The Indonesian Pediatric Society, Jakarta, Indonesia, Department of Pediatrics, Faculty of Medicine, Cipto Mangunkusumo National Central Hospital, Universitas Indonesia, Jakarta, Indonesia |
Background: Indonesia has a high number of COVID-19 cases and mortalities relative to not only among the Asia Pacific region but the world. Children were thought to be less affected by the virus compared to adults. Most of the public data reported combined data between adults and children. The Indonesian Pediatric Society (IPS) was involved in the COVID-19 response, especially in the area of child health. One of IPS's activities is collecting data registries from each of their chapters to provide a better understanding of COVID-19 in children. Objective: The objective of this study was to share the data of suspected and confirmed COVID-19 cases in children from IPS's COVID-19 data registry. Method: This is a retrospective study from the IPS's COVID-19 registry data. We collected the data of COVID-19 in children during March to December 2020 from each of the IPS chapters. We analyzed the prevalence, case fatality rate (CFR), age groups, diagnosis, and comorbidities of the children diagnosed with COVID-19. Result: As of December 21, 2020, there were 35,506 suspected cases of children with COVID-19. In total, there were 522 deaths, with a case fatality ratio (CFR) of 1.4. There were 37,706 confirmed cases with 175 fatalities (CFR 0.46). The highest mortality in confirmed COVID-19 cases was from children ages 10–18 years (42 out of 159 cases: 26%). The most common comorbidity and diagnosis found were malignancy (17.3%) and respiratory failure (54.5%). Conclusion: The CFR of confirmed COVID-19 cases in children in Indonesia is high and should be a major public concern. © Copyright © 2021 Pudjiadi, Putri, Sjakti, Yanuarso, Gunardi, Roeslani, Pasaribu, Nurmalia, Sambo, Ugrasena, Soeroso, Firman, Muryawan, Setyanto, Citraresmi, Effendi, Habibah, Octaviantie, Utami, Prawira, Kaswandani, Alam, Kadafi and Pulungan. |
children; comorbidities; COVID-19; Indonesia; mortality |
adolescent; adult; Article; case fatality rate; cause of death; child; comorbidity; coronavirus disease 2019; human; Indonesia; major clinical study; malignant neoplasm; medical society; mortality; pediatrics; prevalence; questionnaire; register; respiratory failure; retrospective study; sepsis; septic shock |
Frontiers Media S.A. |
22962360 |
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Article |
Q1 |
960 |
4641 |
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176 |
Nakhaei P., Margiana R., Bokov D.O., Abdelbasset W.K., Jadidi Kouhbanani M.A., Varma R.S., Marofi F., Jarahian M., Beheshtkhoo N. |
57226413840;56685900600;56845561300;57208873763;57219274421;7201793587;57199650994;16241593900;57201547070; |
Liposomes: Structure, Biomedical Applications, and Stability Parameters With Emphasis on Cholesterol |
2021 |
Frontiers in Bioengineering and Biotechnology |
9 |
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705886 |
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4 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115653337&doi=10.3389%2ffbioe.2021.705886&partnerID=40&md5=0d6cee3ed2be3a97e6c2beb3d6c668b5 |
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Cipto Mangunkusumo Hospital, The National Referral Hospital, Central Jakarta, Indonesia; Master’s Programme Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Institute of Pharmacy, Sechenov First Moscow State Medical University, Moscow, Russian Federation; Laboratory of Food Chemistry, Federal Research Center of Nutrition, Biotechnology, and Food Safety, Moscow, Russian Federation; Department of Health and Rehabilitation Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al Kharj, Saudi Arabia; Department of Physical Therapy, Kasr Al-Aini Hospital, Cairo University, Giza, Egypt; Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Czech Republic; Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute, Palacký University in Olomouc, Olomouc, Czech Republic; Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Toxicology and Chemotherapy Unit (G401), German Cancer Research Center, Heidelberg, Germany |
Nakhaei, P., School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Margiana, R., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia, Cipto Mangunkusumo Hospital, The National Referral Hospital, Central Jakarta, Indonesia, Master’s Programme Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Bokov, D.O., Institute of Pharmacy, Sechenov First Moscow State Medical University, Moscow, Russian Federation, Laboratory of Food Chemistry, Federal Research Center of Nutrition, Biotechnology, and Food Safety, Moscow, Russian Federation; Abdelbasset, W.K., Department of Health and Rehabilitation Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al Kharj, Saudi Arabia, Department of Physical Therapy, Kasr Al-Aini Hospital, Cairo University, Giza, Egypt; Jadidi Kouhbanani, M.A., Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Czech Republic; Varma, R.S., Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute, Palacký University in Olomouc, Olomouc, Czech Republic; Marofi, F., Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Jarahian, M., Toxicology and Chemotherapy Unit (G401), German Cancer Research Center, Heidelberg, Germany; Beheshtkhoo, N., Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Czech Republic |
Liposomes are essentially a subtype of nanoparticles comprising a hydrophobic tail and a hydrophilic head constituting a phospholipid membrane. The spherical or multilayered spherical structures of liposomes are highly rich in lipid contents with numerous criteria for their classification, including structural features, structural parameters, and size, synthesis methods, preparation, and drug loading. Despite various liposomal applications, such as drug, vaccine/gene delivery, biosensors fabrication, diagnosis, and food products applications, their use encounters many limitations due to physico-chemical instability as their stability is vigorously affected by the constituting ingredients wherein cholesterol performs a vital role in the stability of the liposomal membrane. It has well established that cholesterol exerts its impact by controlling fluidity, permeability, membrane strength, elasticity and stiffness, transition temperature (Tm), drug retention, phospholipid packing, and plasma stability. Although the undetermined optimum amount of cholesterol for preparing a stable and controlled release vehicle has been the downside, but researchers are still focused on cholesterol as a promising material for the stability of liposomes necessitating explanation for the stability promotion of liposomes. Herein, the prior art pertaining to the liposomal appliances, especially for drug delivery in cancer therapy, and their stability emphasizing the roles of cholesterol. © Copyright © 2021 Nakhaei, Margiana, Bokov, Abdelbasset, Jadidi Kouhbanani, Varma, Marofi, Jarahian and Beheshtkhoo. |
cholesterol; compounds; lipids; liposome; stability |
Automobile manufacture; Chemical stability; Controlled drug delivery; Food products; Liposomes; Medical applications; Phospholipids; Plasma stability; Targeted drug delivery; Application parameters; Biomedical applications; Compound; Hydrophilic heads; Hydrophobic tails; Liposome structures; Multi-layered; Phospholipid membrane; Spherical structures; Stability parameters; Cholesterol |
Frontiers Media S.A. |
22964185 |
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Review |
Q1 |
1081 |
3891 |
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222 |
Sirait B., Wiweko B., Jusuf A.A., Iftitah D., Muharam R. |
57222720264;43061741400;57192275719;57195199068;57191492732; |
Oocyte Competence Biomarkers Associated With Oocyte Maturation: A Review |
2021 |
Frontiers in Cell and Developmental Biology |
9 |
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710292 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114781833&doi=10.3389%2ffcell.2021.710292&partnerID=40&md5=0430c11851bd72e4085a2092ee2bfda4 |
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia; Morula IVF Jakarta Clinic, Jakarta, Indonesia; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Human Reproductive, Infertility, and Family Planning Research Cluster, Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Sirait, B., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia, Morula IVF Jakarta Clinic, Jakarta, Indonesia; Wiweko, B., Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproductive, Infertility, and Family Planning Research Cluster, Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Jusuf, A.A., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Iftitah, D., Human Reproductive, Infertility, and Family Planning Research Cluster, Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Muharam, R., Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproductive, Infertility, and Family Planning Research Cluster, Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Oocyte developmental competence is one of the determining factors that influence the outcomes of an IVF cycle regarding the ability of a female gamete to reach maturation, be fertilized, and uphold an embryonic development up until the blastocyst stage. The current approach of assessing the competency of an oocyte is confined to an ambiguous and subjective oocyte morphological evaluation. Over the years, a myriad of biomarkers in the cumulus-oocyte-complex has been identified that could potentially function as molecular predictors for IVF program prognosis. This review aims to describe the predictive significance of several cumulus-oocyte complex (COC) biomarkers in evaluating oocyte developmental competence. A total of eight acclaimed cumulus biomarkers are examined in the study. RT-PCR and microarray analysis were extensively used to assess the significance of these biomarkers in foreseeing oocyte developmental competence. Notably, these biomarkers regulate vital processes associated with oocyte maturation and were found to be differentially expressed in COC encapsulating oocytes of different maturity. The biomarkers were reviewed according to the respective oocyte maturation events namely: nuclear maturation, apoptosis, and extracellular matrix remodeling, and steroid metabolism. Although substantial in vitro evidence was presented to justify the potential use of cumulus biomarkers in predicting oocyte competency and IVF outcomes, the feasibility of assessing these biomarkers as an add-on prognostic procedure in IVF is still restricted due to study challenges. © Copyright © 2021 Sirait, Wiweko, Jusuf, Iftitah and Muharam. |
biomarker; cumulus-oocyte complex (COC); in-vitro fertilization; oocyte competence; oocyte maturation |
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Frontiers Media S.A. |
2296634X |
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Review |
Q1 |
2452 |
946 |
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