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Selisana S.M.G.; Chen X.; Mahfudhoh E.; Bowolaksono A.; Rozaliyani A.; Orihara K.; Kajiwara S. |
Selisana, Shiela Marie Gines (57194495441); Chen, Xinyue (57192119643); Mahfudhoh, Eny (59207100400); Bowolaksono, Anom (57205093224); Rozaliyani, Anna (57203065912); Orihara, Kanami (22234653000); Kajiwara, Susumu (55759474200) |
57194495441; 57192119643; 59207100400; 57205093224; 57203065912; 22234653000; 55759474200 |
Alteration of β-glucan in the emerging fungal pathogen Candida auris leads to immune evasion and increased virulence |
2024 |
Medical Microbiology and Immunology |
213 |
1 |
13 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85197730812&doi=10.1007%2fs00430-024-00795-y&partnerID=40&md5=0ff94a05bae3194789714dcc4d07e21d |
School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Department of Biology, Faculty of Mathematics and Science, University of Indonesia, Depok, 16424, Indonesia; Faculty of Medicine, University of Indonesia, Jakarta, 10430, Indonesia |
Selisana S.M.G., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Chen X., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Mahfudhoh E., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Bowolaksono A., Department of Biology, Faculty of Mathematics and Science, University of Indonesia, Depok, 16424, Indonesia; Rozaliyani A., Faculty of Medicine, University of Indonesia, Jakarta, 10430, Indonesia; Orihara K., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Kajiwara S., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan |
Candida auris is an emerging pathogenic yeast that has been categorized as a global public health threat and a critical priority among fungal pathogens. Despite this, the immune response against C. auris infection is still not well understood. Hosts fight Candida infections through the immune system that recognizes pathogen-associated molecular patterns such as β-glucan, mannan, and chitin on the fungal cell wall. In this study, levels of β-glucan and mannan exposures in C. auris grown under different physiologically relevant stimuli were quantified by flow cytometry-based analysis. Lactate, hypoxia, and sublethal concentration of fluconazole trigger a decrease in surface β-glucan while low pH triggers an increase in β-glucan. There is no inverse pattern between exposure levels of β-glucan and mannan in the cell wall architecture among the three clades. To determine the effect of cell wall remodeling on the immune response, a phagocytosis assay was performed, followed by quantification of released cytokines by ELISA. Lactate-induced decrease in β-glucan leads to reduced uptake of C. auris by PMA-differentiated THP-1 and RAW 264.7 macrophages. Furthermore, reduced production of CCL3/MIP-1⍺ but not TNF-⍺ and IL-10 were observed. An in vivo infection analysis using silkworms reveals that a reduction in β-glucan triggers an increase in the virulence of C. auris. This study demonstrates that β-glucan alteration occurs in C. auris and serves as an escape mechanism from immune cells leading to increased virulence. © The Author(s) 2024. |
Candida auris; Cell wall remodeling; Phagocytosis; Silkworm; β-glucan |
Animals; beta-Glucans; Candida auris; Candidiasis; Cell Wall; Cytokines; Disease Models, Animal; Humans; Immune Evasion; Lactic Acid; Macrophages; Mannans; Mice; Phagocytosis; RAW 264.7 Cells; THP-1 Cells; Virulence; beta glucan; cytokine; fluconazole; interleukin 10; lactic acid; macrophage inflammatory protein 1alpha; mannan; phorbol 12 acetate 13 myristate; tumor necrosis factor; beta glucan; cytokine; mannan; Article; Bombyx mori; Candida auris; controlled study; cytokine release; enzyme linked immunosorbent assay; flow cytometry; fungal cell wall; fungal strain; fungal virulence; fungus growth; hypoxia; immune evasion; immune response; immunocompetent cell; in vivo study; nonhuman; pH; phagocytosis; RAW 264.7 cell line; sublethal concentration; THP-1 cell line; animal; Candida auris; |
Tokyo Institute of Technology, TITECH |
This work was supported by internal research funds from Tokyo Institute of Technology. |
Springer Science and Business Media Deutschland GmbH |
3008584 |
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38967888 |
Article |
Q1 |
1045 |
3924 |
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3 |
Syahrani L.; Asih P.B.S.; Bowolaksono A.; Dwiranti A.; Zubaidah S.; Rozi I.E.; Permana D.H.; Bøgh C.; Bangs M.J.; Grieco J.P.; Achee N.L.; Lobo N.F.; Syafruddin D. |
Syahrani, Lepa (55014440600); Asih, Puji B. S. (6507080729); Bowolaksono, Anom (57205093224); Dwiranti, Astari (55151437900); Zubaidah, Siti (57216572364); Rozi, Ismail E. (57193863994); Permana, Dendi H. (57218188245); Bøgh, Claus (6602120205); Bangs, Michael J. (7007079698); Grieco, John P. (7003422533); Achee, Nicole L. (6603480149); Lobo, Neil F. (35392948100); Syafruddin, Din (6602774565) |
55014440600; 6507080729; 57205093224; 55151437900; 57216572364; 57193863994; 57218188245; 6602120205; 7007079698; 7003422533; 6603480149; 35392948100; 6602774565 |
Impact of a spatial repellent intervention on Anopheles kdr insecticide resistance allele in Sumba, Indonesia |
2024 |
Malaria Journal |
23 |
1 |
31 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85182828683&doi=10.1186%2fs12936-024-04841-1&partnerID=40&md5=d012e123af603ec236833d76ef429e7e |
Doctoral Program, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, Indonesia; Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, Indonesia; Doctoral Program, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia; Doctoral Program of Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; The Sumba Foundation, Public Health and Malaria Control, Sumba, Indonesia; Public Health and Malaria Control, PT Freeport Indonesia, International SOS, Mimika, Indonesia; Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, IN, United States; Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia; Hasanuddin University Medical Research Center (HUMRC), Makassar, Indonesia |
Syahrani L., Doctoral Program, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, Indonesia, Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia; Asih P.B.S., Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia; Bowolaksono A., Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, Indonesia; Dwiranti A., Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, Indonesia; Zubaidah S., Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia; Rozi I.E., Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia, Doctoral Program, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia; Permana D.H., Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia, Doctoral Program of Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Bøgh C., The Sumba Foundation, Public Health and Malaria Control, Sumba, Indonesia; Bangs M.J., Public Health and Malaria Control, PT Freeport Indonesia, International SOS, Mimika, Indonesia; Grieco J.P., Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, IN, United States; Achee N.L., Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, IN, United States; Lobo N.F., Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, IN, United States; Syafruddin D., Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia, Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia, Hasanuddin University Medical Research Center (HUMRC), Makassar, Indonesia |
Background: The emergence of insecticide resistance and outdoor transmission in malaria-endemic areas underlines the urgent need to develop innovative tools, such as spatial repellents (SR), that may circumvent this residual transmission. With limited options for effective insecticides, regular resistance monitoring is warranted for selecting and using appropriate tools. This study evaluates the pyrethroid knockdown resistance (kdr) allele before and after implementing a transfluthrin-based spatial repellent (SR) intervention in placebo-treated clusters. Methods: This study looks at the frequency distribution of the kdr allele in Sumba Island from June 2015 to August 2018. Insecticide susceptibility tests were carried out on female Anopheles sp. aged 3–5 days against permethrin 21.5 μg/ml, deltamethrin 12.5 μg/ml, and transfluthrin 10 μg/ml using CDC bottle assay. PCR sequencing of representative samples from adult mosquito collections and insecticide tests revealed the presence of kdr mutations (L1014F and L1014S) in the VGSC gene. Results: A total of 12 Anopheles species, Anopheles tesselatus, Anopheles. aconitus, Anopheles barbirostris, Anopheles kochi, Anopheles annularis, Anopheles maculatus, Anopheles sundaicus, Anopheles flavirostris, Anopheles balabacensis, Anopheles indefinitus, Anopheles subpictus, and Anopheles vagus were analysed. Anopheles vagus and An. sundaicus predominated in the larval populations. Susceptibility assays for all insecticides identified fully susceptible phenotypes in all species examined. Anopheles increasing frequency of kdr mutant alleles during the 3 year SR deployment was observed in both SR-treated and placebo areas, a statistically significant increase occurred in each arm. However, it is unclear how significant SR is in causing the increase in mutant alleles. The L1014S, knockdown resistance east type (kdr-e) allele was detected for the first time among the mosquito samples in this study. The L1014F, knockdown resistance west type (kdr-w) allele and heteroduplex form (wild-type—mutant) were found in almost all Anopheles species examined, including An. vagus, An. aconitus, An. subpictus, An. tesselatus, An. annularis, An. flavirostris and An. sundaicus. Conclusion: The presence of fully susceptible phenotypes over time, along with an increase in the frequency distribution of the L1014F/S mutations post-intervention, suggest drivers of resistance external to the study, including pyrethroid use in agriculture and long-lasting insecticidal nets (LLINs). However, this does not negate possible SR impacts that support resistance. More studies that enable the comprehension of possible SR-based drivers of resistance in mosquitoes need to be conducted. © 2024, The Author(s). |
Kdr; L1014F; L1014S; Pyrethroid; Resistance; VGSC |
Alleles; Animals; Anopheles; Cyclopropanes; Female; Fluorobenzenes; Indonesia; Insecticide Resistance; Insecticides; Permethrin; deltamethrin; insect repellent; permethrin; placebo; transfluthrin; unclassified drug; cyclopropane derivative; fluorobenzene; insecticide; transfluthrin; allele; Anopheles; Anopheles annularis; Anopheles balabacensis; Anopheles barbirostris; Anopheles flavirostris; Anopheles indefinitus; Anopheles kochi; Anopheles maculatus; Anopheles subpictus; Anopheles sundaicus; Anopheles tesselatus; Anopheles vagus; Anopheles. aconitus; Article; controlled study; gene; gene frequency; gene knockdown; gene mutation; genetic susceptibility; Indonesia; insecticide resistance; kdr gene; nonhuman; phenotype; polymerase chain reaction; spatial analysis; allele; animal; female; ge |
District health departments of Southwest and West Sumba; East Nusa Tenggara Province; Eijkman Research Center for Molecular Biology; Government of Indonesia National Research and Innovation Agency; Health Research Organization; Bill and Melinda Gates Foundation, BMGF; University of Notre Dame, ND, (OPP1081737); Badan Riset dan Inovasi Nasional, BRIN; Universitas Hasanuddin; Ministry of Health of the Republic of Indonesia |
Funding text 1: The authors are grateful for the support of the Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia; The University of Hasanuddin, Makassar, Indonesia; the Ministry of Health Republic of Indonesia; District health departments of Southwest and West Sumba, and East Nusa Tenggara Province. We appreciate the contribution of |
BioMed Central Ltd |
14752875 |
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38254131 |
Article |
Q1 |
1237 |
2935 |
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4 |
Rahayu D.; Meutia A.P.; Priyatini T.; Suratih N.M.D.; Maidarti M.; Harzif A.K.; Muharam R.; Pratama G. |
Rahayu, Deniswari (57208300474); Meutia, Alfa Putri (57203368133); Priyatini, Tyas (57192265423); Suratih, Ni Made Desy (26634582200); Maidarti, Mila (56320510400); Harzif, Achmad Kemal (57191493435); Muharam, R. (57191492732); Pratama, Gita (58162878900) |
57208300474; 57203368133; 57192265423; 26634582200; 56320510400; 57191493435; 57191492732; 58162878900 |
Association of hirsutism and anthropometric profiles with sexual dysfunction and anxiety levels in infertile Indonesian women with polycystic ovarian syndrome |
2024 |
Middle East Fertility Society Journal |
29 |
1 |
19 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85189636536&doi=10.1186%2fs43043-024-00178-6&partnerID=40&md5=22df206c1e005692cbf08891765f633a |
Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Urogynecology and Reconstructive Pelvic Surgery Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Reproductive Endocrinology Division, Department of Obstetrics and Gynecology, Persahabatan General Hospital, Jakarta, Indonesia; Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Rahayu D., Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Meutia A.P., Urogynecology and Reconstructive Pelvic Surgery Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Priyatini T., Urogynecology and Reconstructive Pelvic Surgery Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Suratih N.M.D., Reproductive Endocrinology Division, Department of Obstetrics and Gynecology, Persahabatan General Hospital, Jakarta, Indonesia; Maidarti M., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Harzif A.K., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Muharam R., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Pratama G., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Background: Hyperandrogenism is frequently found in polycystic ovary syndrome (PCOS) and contributes to physical manifestations like hirsutism and obesity, along with infertility. This condition can result in anxiety, depression, and body image disorders, potentially leading to sexual dysfunction. The objective of this investigation was to assess the correlation among hirsutism, anthropometric characteristics, sexual dysfunction, and anxiety levels among infertile Indonesian women diagnosed with PCOS. Methods: From December 2021 to December 2022, a cross-sectional study was undertaken involving 71 infertile women diagnosed with PCOS at Yasmin Clinic, Dr. Cipto Mangunkusumo General Hospital in Jakarta, Indonesia. Hirsutism was assessed using the modified Ferriman-Gallwey (mFG) score; the anthropometric profile was assessed using BMI and waist-to-hip ratio. The assessment of sexual dysfunction was conducted using the Female Sexual Function Index (FSFI) questionnaire, while the evaluation of anxiety levels utilized the HAM-A questionnaires. Results: In this study, it was discovered that 53.3% of subjects experienced sexual dysfunction. However, there was no statistically significant relationship between hirsutism, anthropometric profile, and sexual dysfunction score in infertile women with PCOS (p > 0.05). Analysis of the overall FSFI domain score revealed that lubrication and satisfaction were lower in obese patients (p = 0.02 and p = 0.03), but this did not contribute to an overall sexual dysfunction score. Also, we found that subjects who experienced sexual dysfunction had a higher anxiety score (p < 0.005), with correlation analysis showing that Ferriman-Gallwey (FG) scores have a significant positive correlation with anxiety. Conclusion: There is no correlation between hirsutism, anthropometric profile, and sexual dysfunction in infertile Indonesian women diagnosed with PCOS. However, hirsutism could play a role in causing anxiety in Indonesian PCOS women. Additional investigation is required, as female sexual function is an intricate subject. © The Author(s) 2024. |
Anthropometric profile; Hyperandrogenism; Infertility; PCOS; Polycystic ovarian syndrome; Sexual dysfunction |
contraceptive agent; follitropin; luteinizing hormone; adult; anthropometric parameters; anxiety disorder; arousal; Article; automation; body height; body mass; body weight; correlational study; cross-sectional study; dyspareunia; educational status; employee; employment status; female; female infertility; Female Sexual Function Index; Hamilton Anxiety Scale; hip circumference; hirsutism; human; hyperandrogenism; Indonesian; intra-abdominal fat; laboratory test; lubrication; major clinical study; modified ferriman gallwey score; obese patient; obesity; orgasm; ovary polycystic disease; patient satisfaction; pleasure; prevalence; questionnaire; sexual dysfunction; sexual function; skin disease assessment; social status; subcutaneous fat; transvaginal echography; unemployment; waist circumfe |
Indonesian Reproductive Medicine Research and Training Center; INAREPROMED; PUTI Saintekes 2020, (NKB-2278/UN.2) |
Funding text 1: The authors express gratitude to the Indonesian Reproductive Medicine Research and Training Center (INAREPROMED) and the teams at Yasmin Clinic and Rumah Sakit Dr. Cipto Mangunkusumo Hospital for their invaluable assistance and support throughout this study.; Funding text 2: This study received funding from the PUTI Saintekes 2020 grant, contract number NKB-2278/UN.2.RST/HKP.05.00. |
Springer Science and Business Media Deutschland GmbH |
11105690 |
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Article |
Q3 |
322 |
14459 |
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5 |
Agung F.H.; Sekartini R.; Sudarsono N.C.; Hendarto A.; Werdhani R.A.; Pudjiati S.R.; Hanum L.; Naufal A.; Sawyer S.M. |
Agung, Fransisca Handy (36488930100); Sekartini, Rini (50162572300); Sudarsono, Nani Cahyani (57201862518); Hendarto, Aryono (57204142249); Werdhani, Retno Asti (57189088848); Pudjiati, Sri Retno (58909932500); Hanum, Lathifah (55606285800); Naufal, Affan (58766280400); Sawyer, Susan M (7103253172) |
36488930100; 50162572300; 57201862518; 57204142249; 57189088848; 58909932500; 55606285800; 58766280400; 7103253172 |
Development and validation of the adolescent behavioural change Counselling Assessment Tool in Indonesia |
2024 |
BMC Health Services Research |
24 |
1 |
257 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85186205298&doi=10.1186%2fs12913-024-10582-3&partnerID=40&md5=a579a3309c366dc68cb6f5c74e72efe3 |
Faculty of Medicine, Universitas Pelita Harapan, Jl. Jend. Sudirman No.20, Bencongan, Kelapa Dua, Tangerang, Banten, 15810, Indonesia; Department of Child Health, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Jl. Salemba Raya No.6 Jakarta Pusat, Jakarta, 10430, Indonesia; Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6 Jakarta Pusat, Jakarta, 10430, Indonesia; Faculty of Psychology, Universitas Indonesia, Kampus UI, West Java, Depok, Indonesia; Balaraja Distric Hospital, Jl. Rumah Sakit No 88, Balaraja, Tangerang, Banten, Indonesia; Centre for Adolescent Health, Royal Children’s Hospital and Murdoch Children’s Research Institute, Department of Paediatrics, The University of Melbourne, 50 Flemington Rd, Parkville, 3052, VIC, Australia |
Agung F.H., Faculty of Medicine, Universitas Pelita Harapan, Jl. Jend. Sudirman No.20, Bencongan, Kelapa Dua, Tangerang, Banten, 15810, Indonesia; Sekartini R., Department of Child Health, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Jl. Salemba Raya No.6 Jakarta Pusat, Jakarta, 10430, Indonesia; Sudarsono N.C., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6 Jakarta Pusat, Jakarta, 10430, Indonesia; Hendarto A., Department of Child Health, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Jl. Salemba Raya No.6 Jakarta Pusat, Jakarta, 10430, Indonesia; Werdhani R.A., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6 Jakarta Pusat, Jakarta, 10430, Indonesia; Pudjiati S.R., Faculty of Psychology, Universitas Indonesia, Kampus UI, West Java, Depok, Indonesia; Hanum L., Faculty of Psychology, Universitas Indonesia, Kampus UI, West Java, Depok, Indonesia; Naufal A., Balaraja Distric Hospital, Jl. Rumah Sakit No 88, Balaraja, Tangerang, Banten, Indonesia; Sawyer S.M., Centre for Adolescent Health, Royal Children’s Hospital and Murdoch Children’s Research Institute, Department of Paediatrics, The University of Melbourne, 50 Flemington Rd, Parkville, 3052, VIC, Australia |
Background: Primary care provides an important context to engage adolescents and their families in healthy lifestyles with the goal of reducing future behaviour-related health problems. Developing a valid tool to assess health professionals’ skills in behavioural change counselling is integral to improving the quality of clinical care provided to adolescents in Indonesia. Methods: This work was nested within a project to develop a training program to enhance the behaviour-change counselling of adolescents and their parents by Indonesian primary care professionals. Initial item development was based on the content of the training module and the domain structure of the Behavioral Change Counselling Index (BECCI), a commonly used tool to assess counselling quality in healthcare settings. Expert panels were used to test content validity, while face validity was assessed by a group of trained psychologists. Inter-rater agreement was calculated prior to tests of construct validity and reliability, which involved psychologists rating 125 audio-taped counselling sessions between the health professional and adolescent patients, together with a parent. Results: An initial 13-item tool was developed using a 1–5 Likert scale. Validity and reliability testing resulted in the decision to use a 14-item tool with a 0–3 Likert scale. The scale was found to have a Cronbach’s α coefficient of 0.839 (internal consistency), and there was strong inter-rater agreement (0.931). Conclusion: The assessment tool known as the Adolescent Behavioural Change Counselling Assessment Tool, is a valid and reliable instrument to measure Indonesian health professionals’ behavioural-change counselling skills with adolescent patients. The tool provides an evaluation framework for future interventions that aim to improve health professionals’ skills in addressing adolescent behaviour-related health problems. © The Author(s) 2024. |
Adolescent; Behaviour-change; Counselling skill; Health professionals; Non-communicable diseases; Quality |
Adolescent; Counseling; Delivery of Health Care; Health Behavior; Humans; Indonesia; Psychometrics; Reproducibility of Results; Surveys and Questionnaires; adolescent; counseling; health behavior; health care delivery; human; Indonesia; psychometry; questionnaire; reproducibility |
Maternal Health; Universitas Indonesia, UI, (00/2022, NKB–434/UN2, RST/ HKP.05); Ministry of Health of the Republic of Indonesia |
Funding text 1: The authors thank the Ministry of Health of the Republic of Indonesia, especially the director and the staff of The Directorate of Nutrition, Child and Maternal Health, the provincial health offices from 17 provinces and the clinical psychology team whose expertise greatly contributed to this assessment tool (Dian Oriza, Annisa Rahmalia, Utari Krisnamurthi, Sri Wulandari, Fina Dwi |
BioMed Central Ltd |
14726963 |
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38419005 |
Article |
Q1 |
964 |
4472 |
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6 |
Pangaribuan F.D.; Ham M.F.; Mutmainnah M.; Harahap A.S. |
Pangaribuan, Flora Dameria (59208908500); Ham, Maria Francisca (7004830001); Mutmainnah, Mutiah (58296414300); Harahap, Agnes Stephanie (57218511857) |
59208908500; 7004830001; 58296414300; 57218511857 |
Cytotoxic T-lymphocyte-associated 4 protein expression is associated with a high international prognostic score in advanced-stage classical Hodgkin lymphoma |
2024 |
BMC Research Notes |
17 |
1 |
190 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85197781046&doi=10.1186%2fs13104-024-06853-1&partnerID=40&md5=dad504884f7d240c83610069b5b74ae6 |
Anatomical Pathology Department, Persahabatan Hospital, Jakarta, 13230, Indonesia; Anatomical Pathology Department, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, 10430, Indonesia; Faculty of Medicine Universitas Indonesia, Human Cancer Research Center-Indonesian Medical Education and Research Institute, Jakarta, 10430, Indonesia; Faculty of Medicine, Universitas Muhammadiyah Palembang, Palembang, 30263, Indonesia |
Pangaribuan F.D., Anatomical Pathology Department, Persahabatan Hospital, Jakarta, 13230, Indonesia; Ham M.F., Anatomical Pathology Department, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, 10430, Indonesia, Faculty of Medicine Universitas Indonesia, Human Cancer Research Center-Indonesian Medical Education and Research Institute, Jakarta, 10430, Indonesia; Mutmainnah M., Faculty of Medicine, Universitas Muhammadiyah Palembang, Palembang, 30263, Indonesia; Harahap A.S., Anatomical Pathology Department, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, 10430, Indonesia, Faculty of Medicine Universitas Indonesia, Human Cancer Research Center-Indonesian Medical Education and Research Institute, Jakarta, 10430, Indonesia |
Objective: Twenty percent of all classical Hodgkin lymphoma (CHL) cases relapse and recur, especially in advanced stages with a high International Prognostic Score (IPS). Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is a regulatory molecule that can inhibit the immune response and is related to tumor aggressiveness. This study aimed to determine the relationship between CTLA-4 expression in advanced-stage CHL and IPS, identifying it as a potential therapy target. Results: In advanced-stage CHL, the group with a high IPS exhibited significantly higher mean CTLA-4 expression compared to the group with a low IPS (p = 0.003).The group with Hb level < 10.5 g/dl, leukocyte count > 15,000/µL, lymphocyte count < 8%, albumin level < 4 g/dl, and stage 4 exhibited higher CTLA-4 expression than the other group, although only leukocyte count and stage showed statistical significance (p = 0.004 and p = 0.020). Mean CTLA-4 expression was 239.84 ± 76.36 for nodular sclerosis, 293.95 ± 147.94 for mixed cellularity, 271.4 ± 23.56 for lymphocyte depleted, and 225.2 for lymphocyte-rich subtypes. The results suggest that CTLA-4 expression is associated with adverse prognostic factors in the IPS for advanced-stage CHL, supporting the notion that immune checkpoints play a role in cancer progression. © The Author(s) 2024. |
Advanced-stage; Cytotoxic T-lymphocyte-associated protein 4; Hodgkin lymphoma; International prognostic score |
Adolescent; Adult; Aged; Biomarkers, Tumor; CTLA-4 Antigen; Female; Hodgkin Disease; Humans; Male; Middle Aged; Neoplasm Staging; Prognosis; Young Adult; CTLA4 protein, human; cytotoxic T lymphocyte antigen 4; tumor marker; adolescent; adult; aged; cancer staging; female; genetics; Hodgkin disease; human; immunology; male; metabolism; middle aged; pathology; prognosis; young adult |
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BioMed Central Ltd |
17560500 |
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38978137 |
Article |
Q2 |
534 |
9718 |
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7 |
Fedrizal F.F.; Wijaya I.P.; Abdullah M.; Yamin M. |
Fedrizal, Fenandri Fadillah (57219280233); Wijaya, Ika Prasetya (57193731572); Abdullah, Murdani (7103393434); Yamin, Muhammad (23475706300) |
57219280233; 57193731572; 7103393434; 23475706300 |
Elevated neutrophyl-to-lymphocyte ratioand smoking are associated with chronic total occlusion in patients with ST elevation myocardial infarction |
2024 |
BMC Cardiovascular Disorders |
24 |
1 |
12 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181244917&doi=10.1186%2fs12872-023-03680-3&partnerID=40&md5=9aaf3c2915cdc2583c57f09d4d1e5b80 |
Division of Cardiology, Department of Internal Medicine, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Department of Internal Medicine, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Unit Epidemiology, Department of Internal Medicine, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia |
Fedrizal F.F., Department of Internal Medicine, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Wijaya I.P., Division of Cardiology, Department of Internal Medicine, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Abdullah M., Unit Epidemiology, Department of Internal Medicine, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Yamin M., Division of Cardiology, Department of Internal Medicine, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia |
Background: Atherosclerosis is a progressive disease characterized by the build-up of lipids and connective tissue in the large arteries. Some patients experience chronic total occlusion (CTO). Inflammation plays a key role in the development and complications of atherosclerosis. Neutrophil-to-lymphocyte ratio (NLR) is a marker of systemic inflammation associated with the development of acute coronary syndrome (ACS). We aimed to assess the relationship between NLR and CTO in ACS patients with ST elevated myocardial infarction (STEMI) in Indonesia. Methods: This cross-sectional study was performed with secondary data obtained from patient medical records at Cipto Mangunkusumo National Central Hospital, Jakarta. Inclusion criteria were patients with ACS and STEMI who underwent coronary angiography in 2015–2018. Results: A total of 98 patients were enrolled in the analysis. Most subjects with CTO were male, elderly (> 60), smoking, had no history of diabetes mellitus (DM) or hypertension, no family history of coronary heart disease (CHD), but had a history of ACS and had never consumed statin or antiplatelet medications. Bivariate logistic regression analysis revealed that male gender (PR = 1.820; 95%CI 0.871–3.805; p = 0.025) and smoking (PR = 1.781; 95%CI 1.028–3.086; p = 0.004) were significantly correlated with CTO. Receiver operator characteristic (ROC) curve revealed that higher NLR (≥ 6.42) could predict a CTO diagnosis with positive predictive value (PPV) of 91%. Multivariate analysis revealed that NLR was correlated with an 11.2-fold increase in occurrence of CTO (95%CI 3.250-38.303; p < 0.001). Additionally, smoking was correlated with a 7-fold increase in CTO (95% CI 1.791–30.508; p = 0.006). Conclusion: NLR value of ≥ 6.42 is potentially useful as a marker of CTO in STEMI patients. In addition, smoking increases the risk of CTO in ACS/STEMI patients. © 2023, The Author(s). |
Chronic total occlusion; Coronary angiography; Coronary artery Disease; Myocardial Infarction; Neutrophil-to-lymphocyte ratio |
Acute Coronary Syndrome; Aged; Atherosclerosis; Chronic Disease; Coronary Occlusion; Cross-Sectional Studies; Female; Humans; Inflammation; Lymphocytes; Male; Percutaneous Coronary Intervention; Smoking; ST Elevation Myocardial Infarction; Treatment Outcome; antithrombocytic agent; hydroxymethylglutaryl coenzyme A reductase inhibitor; acute coronary syndrome; adult; age; aged; Article; bivariate analysis; cardiovascular risk; chronic total occlusion; controlled study; coronary angiography; cross-sectional study; diabetes mellitus; diagnostic test accuracy study; disease association; female; human; hypertension; Indonesia; ischemic heart disease; logistic regression analysis; major clinical study; male; medical record; multivariate analysis; neutrophil lymphocyte ratio; predictive value; re |
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BioMed Central Ltd |
14712261 |
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38172732 |
Article |
Q2 |
601 |
8561 |
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9 |
Tarwadi; Pambudi S.; Sriherwanto C.; Sasangka A.N.; Bowolaksono A.; Wijayadikusumah A.R.; Zeng W.; Rachmawati H.; Kartasasmita R.E.; Kazi M. |
Tarwadi (24169949500); Pambudi, Sabar (37115903900); Sriherwanto, Catur (57217729443); Sasangka, Ayu N. (59230814400); Bowolaksono, Anom (57205093224); Wijayadikusumah, Acep R. (57194976916); Zeng, Weiguang (7203023746); Rachmawati, Heni (8601757900); Kartasasmita, Rahmana E. (7801610135); Kazi, Mohsin (56921753300) |
24169949500; 37115903900; 57217729443; 59230814400; 57205093224; 57194976916; 7203023746; 8601757900; 7801610135; 56921753300 |
Inclusion of TAT and NLS sequences in lipopeptide molecules generates homogenous nanoparticles for gene delivery applications |
2024 |
International Journal of Pharmaceutics |
662 |
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124492 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85199330549&doi=10.1016%2fj.ijpharm.2024.124492&partnerID=40&md5=9c578d41204bfdb56f0acbef272c4748 |
Research Center for Vaccines and Drugs, National Agency for Research and Innovation (BRIN), Building 610-611 Puspiptek Area, Banten, Tangerang Selatan, 15314, Indonesia; PT Indomabs Biosantika Utama, Gedung Technology Business and Innovation Centre (TBIC), Pengasinan, Gunung Sindur, Kabupaten Bogor, Jawa Barat, 16340, Indonesia; Research Centre for Applied Microbiology, National Agency for Research and Innovation (BRIN), Building 610-611 Puspiptek Area, Banten, Tangerang Selatan, 15314, Indonesia; Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jawa Barat, Depok, 16424, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Jawa Barat, Depok, 16424, Indonesia; Research and Development Division, PT. Bio Farma, Jl. Pasteur No 28 Bandung, Jawa Barat, 40161, Indonesia; Peter Doherty Institute, The University of Melbourne, 792 Elizabeth St, Melbourne, 3000, VIC, Australia; School of Pharmacy, Bandung Institute of Technology, Jl. Ganesa 10 Bandung, Jawa Barat, 40132, Indonesia; Research Centre of Nano Sciences and Nanotechnology, Bandung Institute of Technology, Jl. Ganesa 10 Bandung 40132, Jawa Barat, Indonesia; Department of Pharmaceutics, College of Pharmacy, POBOX-2457, King Saud University, Riyadh, 11451, Saudi Arabia |
Tarwadi, Research Center for Vaccines and Drugs, National Agency for Research and Innovation (BRIN), Building 610-611 Puspiptek Area, Banten, Tangerang Selatan, 15314, Indonesia, PT Indomabs Biosantika Utama, Gedung Technology Business and Innovation Centre (TBIC), Pengasinan, Gunung Sindur, Kabupaten Bogor, Jawa Barat, 16340, Indonesia; Pambudi S., Research Center for Vaccines and Drugs, National Agency for Research and Innovation (BRIN), Building 610-611 Puspiptek Area, Banten, Tangerang Selatan, 15314, Indonesia; Sriherwanto C., Research Centre for Applied Microbiology, National Agency for Research and Innovation (BRIN), Building 610-611 Puspiptek Area, Banten, Tangerang Selatan, 15314, Indonesia; Sasangka A.N., Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jawa Barat, Depok, 16424, Indonesia; Bowolaksono A., Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Jawa Barat, Depok, 16424, Indonesia; Wijayadikusumah A.R., Research and Development Division, PT. Bio Farma, Jl. Pasteur No 28 Bandung, Jawa Barat, 40161, Indonesia; Zeng W., Peter Doherty Institute, The University of Melbourne, 792 Elizabeth St, Melbourne, 3000, VIC, Australia; Rachmawati H., School of Pharmacy, Bandung Institute of Technology, Jl. Ganesa 10 Bandung, Jawa Barat, 40132, Indonesia, Research Centre of Nano Sciences and Nanotechnology, Bandung Institute of Technology, Jl. Ganesa 10 Bandung 40132, Jawa Barat, Indonesia; Kartasasmita R.E., School of Pharmacy, Bandung Institute of Technology, Jl. Ganesa 10 Bandung, Jawa Barat, 40132, Indonesia; Kazi M., Department of Pharmaceutics, College of Pharmacy, POBOX-2457, King Saud University, Riyadh, 11451, Saudi Arabia |
Purposes: The objective of this study is to develop a versatile gene carrier based on lipopeptides capable of delivering genetic material into target cells with minimal cytotoxicity. Methods: Two lipopeptide molecules, palmitoyl-CKKHH and palmitoyl-CKKHH-YGRKKRRQRRR-PKKKRKV, were synthesized using solid phase peptide synthesis and evaluated as transfection agents. Physicochemical characterization of the lipopeptides included a DNA shift mobility assay, particle size measurement, and transmission electron microscopy (TEM) analysis. Cytotoxicity was assessed in CHO-K1 and HepG2 cells using the MTT assay, while transfection efficiency was determined by evaluating the expression of the green fluorescent protein-encoding gene. Results: Our findings demonstrate that the lipopeptides can bind, condense, and shield DNA from DNase degradation. The inclusion of the YGRKKRRQRRR sequence, a transcription trans activator, and the PKKKRKV sequence, a nuclear localization signal, imparts desirable properties. Lipopeptide-based TAT-NLS/DNA nanoparticles exhibited stability for up to 20 days when stored at 6–8 °C, displaying uniformity with a compact size of approximately 120 nm. Furthermore, the lipopeptides exhibited lower cytotoxicity compared to the poly-L-lysine. Transfection experiments revealed that protein expression mediated by the lipopeptide occurred at a charge ratio ranging from 4.0 to 8.0. Conclusion: These results indicate that the lipopeptide, composed of a palmitoyl alkyl chain and TAT and NLS sequences, can efficiently condense and protect DNA, form stable and uniform nanoparticles, and exhibit promising characteristics as a potential gene carrier with minimal cytotoxicity. © 2024 Elsevier B.V. |
Cytotoxicity; Homogenous nanoparticle; Lipopeptide; NLS; TAT; Transfection |
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King Saud University, KSU; Agency for Research and Innovation; University of Melbourne, UNIMELB, (12/II.7/HK/2023); BRIN, (RSP2024R301) |
We extend our gratitude to Professor David Jackson for his invaluable contribution to lipopeptide synthesis conducted at the Peter Doherty Institute Laboratory, The University of Melbourne, Australia. We also express our appreciation to the support of Research and Innovation Program for Advancement of Indonesia (RIIM) Project Number of 12/II.7/HK/2023 at the Agency for Research and Innovation (BRI |
Elsevier B.V. |
3785173 |
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Article |
Q1 |
906 |
4937 |
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11 |
Harzif A.K.; Andyra A.F.; Sayogo A.; Ummah N.; Puspawardani A.R.; Nurbaeti P.; Wiweko B. |
Harzif, Achmad Kemal (57191493435); Andyra, Azizah Fitriayu (57438092800); Sayogo, Atikah (58945649000); Ummah, Nafi’atul (58945649100); Puspawardani, Aisyah Retno (58944793100); Nurbaeti, Putri (58945649200); Wiweko, Budi (43061741400) |
57191493435; 57438092800; 58945649000; 58945649100; 58944793100; 58945649200; 43061741400 |
Embryo response to aneuploidy through self-correction mechanism: a literature review |
2024 |
Middle East Fertility Society Journal |
29 |
1 |
16 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85188103317&doi=10.1186%2fs43043-024-00176-8&partnerID=40&md5=2ea59e3b8e555f1604df15976d449b84 |
Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Harzif A.K., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Andyra A.F., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sayogo A., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Ummah N., Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Puspawardani A.R., Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Nurbaeti P., Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Wiweko B., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Background: Meiotic and mitotic errors often lead to aneuploidy and mosaicism. In this context, the self-correction mechanism enables the embryo to preferentially retain and preserve euploid cells through processes such as apoptosis, necrosis, or marginalization. This mechanism is thought to minimize the chance of genetic abnormalities during cell development. Materials and methods: A literature search for articles written in English from January 2013 to October 2023 was conducted on PubMed, EBSCO, and Scopus, using the keywords “self-correction,” “self-repair,” “aneuploidy,” “mosaicism,” and “embryo.” Results: A total of 308 articles were collected, out of which 5 retrospective and 1 prospective study were selected based on inclusion criteria. Discussions: Investigations showed that embryos remove chromosomally abnormal cells, supporting the self-correction mechanism. aCGH has been used in 4 studies to demonstrate the presence of self-correction in mosaic embryos. Furthermore, a higher relative viability of polyploidies than complex aneuploidies was observed, suggesting early discrimination against complex aneuploidy, particularly those arising from mitotic origins. However, there are doubts about the reliability of preimplantation genetic testing for aneuploidy at the blastocyst stage, as it may lead to a high rate of false positives and the discarding of "good" embryos. Conclusions: Studies showed a self-correction mechanism in human embryos through the ability to expel abnormal cells. Further investigation is needed to elucidate the underlying mechanisms and determine optimal strategies for preimplantation genetic testing to fully understand and optimize the use of self-correction mechanisms in embryo assessment and selection. © The Author(s) 2024. |
Aneuploidy; Embryo; Mosaicism; Self-correction; Self-repair |
aneuploidy; blastocyst; blastomere; chromosome 19; chromosome aberration; comparative genomic hybridization; cytogenetic analysis; embryo; embryo (anatomy); embryo biopsy; embryo development; false positive result; high throughput sequencing; human; human embryo; mosaicism; polymerase chain reaction; polyploidy; preimplantation genetic screening; Review; single nucleotide polymorphism array; trophectoderm; uniparental disomy; X chromosome |
Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia |
The authors would like to express gratitude to the Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, for supporting this study. |
Springer Science and Business Media Deutschland GmbH |
11105690 |
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Review |
Q3 |
322 |
14459 |
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13 |
Tenggara J.B.; Rachman A.; Prihartono J.; Rachmadi L.; Panigoro S.S.; Heriyanto D.S.; Sutandyo N.; Nasution I.R.; Rahadiati F.B.; Steven R.; Betsy R.; Juanputra S.; Sudoyo A.W. |
Tenggara, Jeffry Beta (26028154300); Rachman, Andhika (15056701600); Prihartono, Joedo (6602605635); Rachmadi, Lisnawati (55062422000); Panigoro, Sonar Soni (56790104300); Heriyanto, Didik Setyo (54420130300); Sutandyo, Noorwati (26028099200); Nasution, Intan Russianna (58856886500); Rahadiati, Familia Bella (57222312428); Steven, Ricci (58808727700); Betsy, Rachelle (58035832700); Juanputra, Samuel (58035300800); Sudoyo, Aru Wisaksono (6507478291) |
26028154300; 15056701600; 6602605635; 55062422000; 56790104300; 54420130300; 26028099200; 58856886500; 57222312428; 58808727700; 58035832700; 58035300800; 6507478291 |
The relationship between high ratios of CD4/FOXP3 and CD8/CD163 and the improved survivability of metastatic triple-negative breast cancer patients: a multicenter cohort study |
2024 |
BMC Research Notes |
17 |
1 |
44 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183752630&doi=10.1186%2fs13104-024-06704-z&partnerID=40&md5=de027f325777bb9ecbe793095eb9ae40 |
Division of Hematology and Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine Universitas Indonesia, Jl. Pangeran Diponegoro No. 71, RW.5, Kec. Senen, Central Jakarta, Jakarta, 10430, Indonesia; Division of Hematology and Medical Oncology, Department of Internal Medicine, MRCCC Siloam Hospital Jakarta, Jakarta, Indonesia; Department of Community Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomical Pathology, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Surgical Oncology, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomical Pathology, Dr. Sardjito Hospital–Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia; Division of Hematology and Medical Oncology, Department of Internal Medicine, Dharmais National Cancer Hospital, Jakarta, Indonesia; Division of Hematology and Medical Oncology, Gatot Soebroto Army Hospital Jakarta, Jakarta, Indonesia; Department of Anatomical Pathology, Gatot Soebroto Army Hospital Jakarta, Jakarta, Indonesia; Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Tenggara J.B., Division of Hematology and Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine Universitas Indonesia, Jl. Pangeran Diponegoro No. 71, RW.5, Kec. Senen, Central Jakarta, Jakarta, 10430, Indonesia, Division of Hematology and Medical Oncology, Department of Internal Medicine, MRCCC Siloam Hospital Jakarta, Jakarta, Indonesia; Rachman A., Division of Hematology and Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine Universitas Indonesia, Jl. Pangeran Diponegoro No. 71, RW.5, Kec. Senen, Central Jakarta, Jakarta, 10430, Indonesia, Division of Hematology and Medical Oncology, Department of Internal Medicine, MRCCC Siloam Hospital Jakarta, Jakarta, Indonesia; Prihartono J., Department of Community Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Rachmadi L., Department of Anatomical Pathology, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Panigoro S.S., Department of Surgical Oncology, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Heriyanto D.S., Department of Anatomical Pathology, Dr. Sardjito Hospital–Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia; Sutandyo N., Division of Hematology and Medical Oncology, Department of Internal Medicine, Dharmais National Cancer Hospital, Jakarta, Indonesia; Nasution I.R., Division of Hematology and Medical Oncology, Gatot Soebroto Army Hospital Jakarta, Jakarta, Indonesia; Rahadiati F.B., Department of Anatomical Pathology, Gatot Soebroto Army Hospital Jakarta, Jakarta, Indonesia; Steven R., Division of Hematology and Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine Universitas Indonesia, Jl. Pangeran Diponegoro No. 71, RW.5, Kec. Senen, Central Jakarta, Jakarta, 10430, Indonesia, Division of Hematology and Medical Oncology, Department of Internal Medicine, MRCCC Siloam Hospital Jakarta, Jakarta, Indonesia; Betsy R., Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Juanputra S., Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sudoyo A.W., Division of Hematology and Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital–Faculty of Medicine Universitas Indonesia, Jl. Pangeran Diponegoro No. 71, RW.5, Kec. Senen, Central Jakarta, Jakarta, 10430, Indonesia, Division of Hematology and Medical Oncology, Department of Internal Medicine, MRCCC Siloam Hospital Jakarta, Jakarta, Indonesia |
Background: Triple-negative breast cancer (TNBC) has been documented as the most aggressive subtype of breast cancer. This study aimed to analyze antitumor and protumor immune activities, and their ratios as significant prognostic biomarkers in metastatic TNBC (mTNBC). Methods: A multicenter cohort study was conducted among 103 de novo mTNBC patients. The expression of CD8 and CD163 was evaluated using immunohistochemistry staining, CD4 and FOXP3 using double-staining immunohistochemistry, and PD-L1 using immunohistochemistry and RT-PCR. Results: Multivariate analysis revealed that high CD4/FOXP3 (HR 1.857; 95% CI 1.049–3.288; p = 0.034) and the CD8/CD163 ratio (HR 2.089; 95% CI 1.174–3.717; p = 0.012) yield significantly improved 1 year overall survival (OS). Kaplan–Meier analysis showed that high levels of CD4 (p = 0.023), CD8 (p = 0.043), CD4/FOXP3 (p = 0.016), CD8/FOXP3 (p = 0.005), CD8/CD163 (p = 0.005) ratios were significantly associated with higher rate of 1 year OS. Furthermore, 1 year OS was directly correlated with antitumor CD4 (R = 0.233; p = 0.018) and CD8 (R = 0.219; p = 0.026) and was indirectly correlated with protumor CD163 and FOXP3 through CD4/FOXP3 (R = 0.282; p = 0.006), CD4/CD163 (R = 0.239; p = 0.015), CD8/FOXP3 (R = 0.260; p = 0.008), and CD8/CD163 (R = 0.258; p = 0.009). Conclusion: This is the first study to demonstrate that high levels of CD4/FOXP3 and CD8/CD163 significantly improved the 1 year OS in de novo mTNBC patients. Thus, we recommend the application of these markers as prognosis determination and individual treatment decision. © 2024, The Author(s). |
CD163; CD4; CD8; FOXP3; Metastatic; Survival; Triple-negative breast cancer |
B7-H1 Antigen; CD8-Positive T-Lymphocytes; Cohort Studies; Forkhead Transcription Factors; Humans; Lymphocytes, Tumor-Infiltrating; Neoadjuvant Therapy; Triple Negative Breast Neoplasms; forkhead transcription factor; FOXP3 protein, human; programmed death 1 ligand 1; CD8+ T lymphocyte; clinical trial; cohort analysis; genetics; human; metabolism; multicenter study; neoadjuvant therapy; pathology; triple negative breast cancer; tumor associated leukocyte |
Department of Anatomical Pathology; Department of Internal Medicine, University of Utah; Department of Radiology, Weill Cornell Medical College |
The authors gratefully acknowledged all staff of the medical record, the Department of Radiology, the Department of Hematology and Medical Oncology, the Department of Anatomical Pathology (Deny Suprihatin), and the Clinical Epidemiology Division, Department of Internal Medicine (Utami Susilowati). |
BioMed Central Ltd |
17560500 |
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38308298 |
Article |
Q2 |
534 |
9718 |
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14 |
Wiguna T.; Dirjayanto V.J.; Maharani Z.S.; Faisal E.G.; Teh S.D.; Kinzie E. |
Wiguna, Tjhin (24367785700); Dirjayanto, Valerie Josephine (57404619000); Maharani, Zhahna Siti (58854585000); Faisal, Emir Gibraltar (57783485400); Teh, Sylvie Dominic (57932340200); Kinzie, Erik (8603207100) |
24367785700; 57404619000; 58854585000; 57783485400; 57932340200; 8603207100 |
Mental health disturbance in preclinical medical students and its association with screen time, sleep quality, and depression during the COVID-19 pandemic |
2024 |
BMC Psychiatry |
24 |
1 |
85 |
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0 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183663019&doi=10.1186%2fs12888-024-05512-w&partnerID=40&md5=54cb1c9276b6b0717d89be4550cf29a1 |
Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom; Department of Psychiatry, Tulane University School of Medicine, New Orleans, United States |
Wiguna T., Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Dirjayanto V.J., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom; Maharani Z.S., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faisal E.G., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Teh S.D., Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Kinzie E., Department of Psychiatry, Tulane University School of Medicine, New Orleans, United States |
Background: Affected by various hurdles during COVID-19, preclinical medical students are at an elevated risk for mental health disturbances. However, the effects of modern mental health problems on preclinical medical students have not been adequately researched. Thus, this study was aimed to identify the proportions and implications of current mental health problems for depression, sleep quality and screen time among Indonesian medical preclinical students during the COVID-19 pandemic. Methods: This cross-sectional study was conducted using crowdsourcing between October 2020 and June 2021. During the study period, 1,335 subjects were recruited, and 1,023 datasets were identified as valid. General Health Questionnaire-12 (GHQ-12) was used to measure current mental health disturbances (categorized as without current mental health disturbances, psychological distress, social dysfunction, or both). The Patient Health Questionnaire-9 (PHQ-9) was used to assess depression, the Pittsburgh Sleep Quality Index (PSQI) was employed to assess sleep quality, and a questionnaire devised for this study was used to assess screen time length per day. Multivariate data analysis was conducted using SPSS version 24 for Mac. Results: According to the findings, 49.1% of the 1,023 participants had current mental health disturbances: 12.8% had psychological distress, 15.9% had social dysfunction, and the rest (20.4%) had both psychological distress and social dysfunction. The statistical analysis provided strong evidence of a difference (p < 0.001) between the medians of depression and sleep quality with at least one pair of current mental health disturbance groups, but the difference for screen time was not significant (p = 0.151). Dunn’s post-hoc analysis showed that groups without current mental health problems had significantly lower mean ranks of depression and sleep quality compared to groups that had current mental health problems (p < 0.001). Conclusion: Current mental health disturbances during the COVID-19 pandemic were significantly associated with preclinical medical students’ depression and sleep quality in preclinical medical students. Thus, mental health programs for this specific population should be tailored to integrate mindfulness therapy, support groups, stress management, and skills training to promote mental wellbeing. © 2024, The Author(s). |
COVID-19; Depression; Indonesia; Medical students; Mental health; Screen time; Sleep quality |
COVID-19; Cross-Sectional Studies; Depression; Humans; Mental Health; Pandemics; Screen Time; Sleep Quality; Students, Medical; adolescent; adult; Article; clinical feature; controlled study; coping skills training; coronavirus disease 2019; cross-sectional study; crowdsourcing; depression; disease association; distress syndrome; female; General Health Questionnaire-12; health program; human; Indonesian; intervention study; major clinical study; male; medical student; mental disease; pandemic; Patient Health Questionnaire 9; Pittsburgh Sleep Quality Index; psychological well-being; screen time; sleep quality; social disability; stress management; coronavirus disease 2019; depression; medical student; mental health; pandemic; screen time; sleep quality |
Executive Student Bodies across 80 universities; Humam Faras Arbangi; Indonesian Medical Students’ Association; PUTI; Universitas Indonesia, UI, (NKB-605/UN2.RST/HKP.05.00/2022) |
Funding text 1: The study was funded by the PUTI Grant Universitas Indonesia with contract number NKB-605/UN2.RST/HKP.05.00/2022. The funding body was not involved in the design, data collection, analysis, data interpretation, or writing of the manuscript. ; Funding text 2: The authors would like to extend their appreciation to Jasmine Wardhana, Indira Laksmi, Ilaisya Azis, and Humam Faras Arbangi |
BioMed Central Ltd |
1471244X |
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38297243 |
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